Phaleria Macrocarpa’s Extract Inhibits Autophagy Probably Through TNF-α in HUVEC Cell Culture (original) (raw)
Related papers
2018
Introduction : Preeclampsia is a major cause in both maternal and perinatal mortality and morbidity. The etiopathogenesis of preeclampsia remains unclear but endothelial dysfunction plays important role. Stress oxidative in placenta produces free radicals such as superoxide anions (O2-) and H2O2, trophoblast debris, pro-inflammatory cytokines, and antiangiogenic factors which are thought to cause vascular endothelial dysfunction and causing excessive maternal inflammatory responses. Tumor Necrosis Factor-α (TNF-α) is considered as one of the potentially specific markers for preeclampsia. Aim : This study aimed to determine the effects of Phaleria macrocarpa (Scheff.) Boerl Extract on inflammation in endothelial cells by measuring the TNF-α level In preeclampsia-induced HUVEC. Method : HUVEC (Human Umbilical Vein Endothelial Cell) culture is an in vitro model widely used to study the pathogenesis of preeclampsia. Phaleria macrocarpa (Scheff.) Boerl also known as Mahkota Dewa is widel...
International Journal of Pharmacy and Pharmaceutical Sciences, 2017
Phaleria macrocarpa (Scheff) Boerl is a medicinal plant that originates from West Papua, Indonesia. The fruit of this plant is known to contain numerous different compounds that produce different bioactivities. Many of these bioactivities are related to women pathological conditions. The purpose of this review is to evaluate the effect of P. macrocarpa fruit extract in the management of these conditions. Different studies have proven that P. macrocarpa extract helps regulate hormone imbalance in women with problems relating to their menstruation cycle, especially during premenstrual syndrome. It helps alleviate symptoms of primary dysmenorrhea and endometriosis through its bioactivity as anti-inflammation, apoptosis inducer, anti-angiogenic and anti-oxidant agent. P. macrocarpa fruit extract also showed selective anti-proliferative, anti-inflammatory, and anti-angiogenic activity on breast and cervical cancer cells. It regulates cancer cell progression through numerous different pat...
Background: Endometriois represents a gynecological disease that still becomes an issue in community. Phaleria macrocarpa is a plant native to Indonesia that contains an antioxidant substance, which may serve as apoptotic modulator and useful for angiogenesis. Objective: This study aims to evaluate the effects of flavonoid isolates from P. macrocarpa (PM) on the development of granulomas, apoptosis, proliferation, and angiogenesis of the disease. Material and methods: Total thirty mice (Mus musculus) were categorized into six groups, including the normal group (without any treatment), EMT (endometriosis) group, and EMT group treated with PM flavonoid isolates. Identification of the active compounds of P. macrocarpa was done using LC-HRMS. Measurement of granuloma scores and vascular density was done histologically. Apoptosis and proliferation analysis was performed by immunohistochemical techniques. Results: There was an increase in granulomas, proliferation, and apoptosis in the peritoneal tissues of the endometriosis model. This change can be normalized by extract of P. macrocarpa. Conclusion: We concluded that the flavonoid isolates from P. macrocarpa can suppress the growth of endometriosis lesions through normalization of proliferation and apoptosis. Thus, the P. macrocarpa flavonoid can be used as an alternative to inhibit the development of endometriosis.
Journal of Advanced Zoology
Endometriosis is a inflamatory disease especially in the state of reproductive age. Phaleria macrocarpa is a plant that has a population in Papua, Irian Jaya. Endometriosis therapies available have still many weakness, including being expensive, only relieving pain, and continuous treatment to prevent endometriosis from recurring. The fruit and seeds of the Phaleria macrocarpa have a wide range of important biological activities in the form of extracts such as antimicrobial, anti-inflammatory, and antioxidant activities. This aim of this was to analyze the effect of flavonoid from extract of phaleria macrocarpa on IL-2, IL-17A, and IL-33 levels in endometriosis mice. This research is using total of 40 female mice (Mus musculus) model endometriosis were grouped into 8 groups, including of healthy mice (without any treatment), the endometriosis group, and the endometriosis group treated flavonoids of Phaleria macrocarpa with dose of 3.75 mg/day; 7.5 mg/day; 11.2 mg/day; 15 mg/day. The...
Journal of Toxicology
Phaleria macrocarpa is one of the Indonesian herbal plants which has been shown to have a hepatoprotective effect. This study was conducted to evaluate the protective effect of water extract of mahkota dewa (Phaleria macrocarpa) in liver fibrosis and to elucidate its mechanism of action. Male Sprague-Dawley rats were treated with carbon tetrachloride (CCl4) for 8 weeks to induce liver fibrosis. Rats were randomly divided into 6 groups (n=5), i.e., control group, CCl4 group, CCl4 + NAC group, CCl4 + various doses of water extract of Phaleria macrocarpa (50, 100, and 150 mg/kg body weight). Aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), liver histopathology, malondialdehyde (MDA), ratio GSH/GSSG, Tumor Necrosis Factor- (TNF-) α, and Transforming Growth Factor- (TGF-) β1 were analyzed. This study demonstrated that water extract of Phaleria macrocarpa and NAC significantly protected CCl4-induced liver injury as demonstrated by reduced AST, ...
Open Access Macedonian Journal of Medical Sciences
AIM: This study was aimed to determine the antifibrotic activity of Phaleria macrocarpa (PM) extract in liver fibrosis (LF) and its possible mechanism in the rat model. METHODS: Sprague Dawley male rats were injected with 2 mL/kg BW of carbon tetrachloride intraperitoneally twice a week for 2 weeks, followed by 1 mL/kg BW for 6 weeks. Afterward, the treatments began from the 3rd week: Silymarin 100 mg/kg BW/day, standardized PM extract (Proliverenol) 75 or 150 mg/kg BW/day orally. Rats were sacrificed in the 8th week. Blood and liver were collected to analyze liver function, liver damage and fibrosis marker, oxidative stress markers, pro-fibrogenic cytokine, and antifibrotic marker. RESULTS: Our study showed that the treatment of silymarin and PM resulted in the normalized activity of liver function, followed by the amelioration of oxidative stress, demonstrated by the decreased malondialdehyde levels and an increased ratio of glutathione and glutathione disulfide. All markers exami...
Evaluation of Subacute Toxicity of Flavonoid from Phaleria Macrocarpa Fruit Extract in Mice
Journal of Advanced Zoology
Background: Traditional medicine and supplements including Phaleria macrocarpa or Mahkota Dewa are extensively utilised. Rape fruit of phaleria macrocarpa contains flavonoid as a secondary metabolite that has a beneficial effect on endometriosis treatment and other illnesses. The purpose of this study was to look into the subacute toxicity of flavonoid from phaleria macrocarpa fruit extract on the brain and heart of BALB/c female mice. Method: The BALB/c female mice aged 6-8 weeks with a body weight of 20-30gr were divided into four groups and treated for 28 days. The control group received simply water, whereas the treatment group received flavonoid at doses of 500mg/kg/day, 1000mg/kg/day, and 2000mg/kg/day. At the end of the experiment, the mice were sacrificed and important organs were extracted and assessed. The organ weight was assessed using macroscopy, and the brain and heart were assessed using microscopy (histopathological abnormalities). Result: The BALB/c female mice trea...