Antioxidant Studies of Aqueous Extract of Phyllanthus Reticulatus Poir (original) (raw)
Related papers
2008
Summary The herb Phyllanthus reticulatus is commonly known as potato bush, which is widely used in the indigenous system of medicine for the treatment of diarrhoea, diabetes, obesity and burns. The methanolic extract of the plant was studied for its in vitro free radical scavenging activity in different methods viz DPPH radical scavenging assay, ABTS radical scavenging assay, Ophenanthroline assay, lipid peroxidation assay, nitric oxide scavenging assay, superoxide scavenging assay, total antioxidant and non-enzymatic haemoglobin glycosylation assay. The results were analyzed statistically by regression method. Its antioxidant activity was estimated by IC50 value and the values were 14.31 µgm/ml (DPPH radical scavenging), 10.51 µgm/ml (ABTS radical scavenging), 22.91µgm/ml (O-phenanthroline assay), 32.16 µgm/ml (lipid peroxidation), 90.51µgm/ml (nitric oxide scavenging) and 4.18µgm/ml (superoxide scavenging). In total antioxidant capacity assay, 1 mg of extract is equivalent to 41.3...
Antioxidant activities of the standardized water extract from fruit of phyllanthus emblica linn
Songklanakarin Journal of Science and Technology, 2010
Phyllanthus emblica Linn. is widely used in Thai traditional medicine for treatment of various diseases. The fruit of P. emblica is known as a rich source of vitamin C, and also contains a mixture of phenolic compounds. In this study, the standardized water extract of P. emblica fruit was prepared according to Thai Herbal Pharmacopoeia. Total polyphenol contents of the extract were equivalent to 34.22±1.74 g gallic acid/100g extract. Antioxidant activities of the P. emblica extract were evaluated by several methods, including DPPH and ABTS •+ radical scavenging assays and FRAP assays. The results showed that the extract has an ability of scavenging radicals generated by both DPPH and ABTS •+ . Similar to Trolox, the water extract of P. emblica fruit also had a ferric reducing property. Additionally, the extract effectively inhibited H 2 O 2 - induced free radical production in human myeloleukemic U937 cells as measured by 2,7-DCF-DA. The results imply that the fruits of P. emblica a...
IN VITRO ANTIOXIDANT ACTIVITIES OF PHYLLANTHUSAMARUS HERBS
In this study, measure antioxidant potential of P. amarus by DPPH radical scavenging activity, Superoxide radical scavenging activity and Hydroxyl radical scavenging activity method using ether, aqueous and ethanol extract in different propagation also reference compound as Ascorbic Acid. In DPPH radical scavenging activity, ethanol extract show higher percentage inhibition 93.50% at 250µg/mL. Higher percentage inhibition of all three activities i.e. DPPH, SOD and hydroxyl at 250 µg/mL by ethanol extract is 96.50 % with reference compound i.e. Ascorbic Acid-97.00%.
International Journal of Pharmacy and Pharmaceutical Sciences
Objective: The modern research is directed towards finding naturally occurring antioxidants of plant origin. To search for new sources of safe and inexpensive antioxidants, present study was undertaken to quantify total phenols and flavonoids and, evaluation and correlation of antioxidant potential of aqueous extract of Phyllanthus amarus Schum & Thonn, (PAAEt) belongs to Euphorbiace family under in vitro condition. Methods: Determination of total phenolic and flavonoid contents was performed by UV-visible spectrophotometer. The antioxidant potential of P. amarus was tested with different antioxidant test systems; inhibition of lipid peroxidation (LPO), scavenging of 1,1-Diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, superoxide and nitric oxide radicals with reducing power. Results: The plant extract has appreciable amounts of total phenolic compounds (120±2.0 mg GA eq. g-1) and flavonoids (3.02±0.13 mg Q eq. g-1) of the extract. PAAEt exhibited effective and strong antioxidant activity in dose-dependent manners in inhibiting LPO (IC50 623.4 μg/ml) and scavenging of free radicals such as DPPH (IC50 200.2 μg/ml), hydroxyl (IC50 280.6 μg/ml), superoxide (IC50 201.5 μg/ml), nitric oxide (IC50 408.5 μg/ml) with reducing power (IC50 500.0 μg/ml). PAAEt showed effective and strong positive correlation with reducing power (r 2 = 0.972) and LPO inhibition (r 2 Conclusion: Presence of high amount of phenolic and flavonoid compounds and appreciable amounts of IC50 for various free radicals and inhibition of LPO and reducing power with significant strong positive correlation for scavenging of free radicals, LPO inhibition and reducing power indicated that PAAEt has strong antioxidant potential. = 0.964) and free-radicals (P<0.05).
International Journal of Green Pharmacy, 2010
In the present study, we determined the antioxidant activity of the entire plant of Phyllanthus reticulatus by performing different in vitro antioxidant assays, including 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) radical scavenging, β-carotene bleaching, superoxide anion radical scavenging, reducing power and metal chelating assay at different concentrations (100, 200 and 400 μg/ml). The entire plant powder of P. reticulatus shows good antioxidant activity of about 90.0% when compared with standard Butylated Hydroxy Toluene (BHT) (85%) at a concentration of 400 μg/ml. Results obtained reveal that methanolic extracts of entire plant of P. reticulatus possess higher antioxidant activity when compared with ethanolic extracts. Thus, this study suggests that P. reticulatus plant can be used as a potent source of natural antioxidants.
An in-vitro investigation of the antioxidant activity of Phyllanthus amarus
The aim of this work was to investigate the total phenolic content and antioxidant potential of aqueous extract of Phyllanthus amarus. The aqueous extract of Phyllanthus amarus whole plant was prepared then systematically investigated by various antioxidant assays, including: DPPH-(1, 1-diphenyl-2-picrylhydrazyl radical), Nitric oxide radical inhibition activity, and β-carotene-linoleate method, compared with ascorbic acid and rutin. Its total phenolic content was also measured by Folin-Ciocalteu colorimetric method. P. amarus aqueous extract exhibited effective antioxidant activity in dose-dependent manners and its IC 50 values were calculated as 4.21±0.379, 426±0.512, 126±0.348 for DPPH, Nitric oxide radical inhibition activity, and β-carotene-linoleate assay respectively. Its total phenolic content was 52.71± 0.214 mg GAE /g. P. amarus aqueous extract has effective in vitro antioxidant activity which may attribute to its total phenolics, among which phyllanthin and hypophyllanthin can be considered as the active components. The pharmacological effects or healthcare functions of P. amarus may result from the synergistic effects caused by the combination of its components and its antioxidant effect plays an important role in the synergistic effects.
ANTIOXIDANT ACTIVITY OF ETHANOLIC AND AQUEOUS EXTRACT OF PHYLLANTHUS NIRURI –INVITRO
Medicinal plants are solving the health problems of world since decades and currently attracting the phenomenal interest at both the national and international level. Recently use of naturally occurring antioxidant for medicine or as a food material has been increased to avoid the side effects of synthetic antioxidants. Phyllanthus niruri (Linn) member of family Euphorbiaceae is one of the important medicinal plant, however less researched for its antioxidant activity and active ingredients. In this study antioxidant activity of ethanolic and aqueous extract of Phyllanthus niruri was analyzed with DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical and Hydrogen peroxide scavenging methods. Ascorbic acid, a standard antioxidant was used as control during the entire study period. The results indicate that ethanolic and aqueous extracts of Phyllanthus niruri are showing antioxidant activity with both the methods, however ethanolic extract showed high scavenging activity in comparison to aqueous extract with both the methods.
The Scientific World Journal, 2014
In present study free radical scavenging potential of aerial parts and root ofPhyllanthus fraternuswas investigated. Extraction was done in water and ethanol. Total antioxidant capacity was measured by DPPH free radical scavenging method; ethanolic extract of aerial part was most potent in activity with 50% inhibition at 258 μg/mL concentration. Lipid peroxidation (LPO) was measured in terms of thiobarbituric acid-reactive substances (TBARS) by using egg-yolk homogenates as lipid-rich media with EC50of aerial part (ethanolic) 1522 μg/mL which was found to be most active. Superoxide (SO) radical scavenging activity was measured using riboflavin-light-nitroblue tetrazolium assay. Ethanolic and aqueous extract of both aerial part and root was almost similar in superoxide radical scavenging activity. Reducing power was determined on the basis ofFe3+-Fe2+transformation in the presence of extract. Total phenolic and flavonoid contents were also measured by spectroscopic method. Results sh...
Quality assessment and evaluation of in-vitro antioxidant potential of Phyllanthus emblica
2015
Phyllanthus emblica L. syn. Emblica officinalis Gaertn. commonly known as amla is a well known drug in India having nutritional value with various health related benefits and employed in various herbal formulations due to the presence of high levels of vitamin C along with valuable phenolic constituents and amino acids. In the present study, quality assessment of amla fruit was carried out by studying macro and microscopic characters along with physicochemical tests for the identification of drug. Chemoprofiling was achieved with thin layer chromatography by optimizing the mobile phase for different extracts. Total phenolic contents and in-vitro antioxidant efficacy was also determined as the drug is well known for protective role in human body as antioxidant. The contaminant evaluation was carried out by analyzing the samples for the determination of heavy metals, pesticides and aflatoxins. The results of quality control and anatomical studies found useful for its identification wi...
In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India
LWT - Food Science and Technology, 2007
This study was designed to examine the in vitro antioxidant activities of the methanol extracts of four Helichrysum species (Helichrysum noeanum Boiss., H. chionophilum Boiss. & Bal., H. plicatum DC. subsp. plicatum, H. arenarium (L.) Moench. subsp. aucheri (Boiss.) Davis & Kuphicha). The extracts were screened for their possible antioxidant activitiy by two complementary test systems, namely DPPH free radical-scavenging and b-carotene/linoleic acid systems. In the first case, non-polar subfractions of the methanol extracts of Helichrysum species studied did not show any antioxidant activity, while the most active one was H. chionophilum (IC 50 ¼ 40.5 lg/ml) among the polar subfractions. In the b-carotene/linoleic acid test system, inhibition rates of the oxidation of linoleic acid of H. noeanum and H. arenarium were very close to each other. The inhibition rate of the synthetic antioxidant BHT was determined to be 96%. Since the polar extracts of Helichrysum species dealt with here exhibited interesting antioxidant activities when compared to BHT, we believe that it would be useful to take the results into consideration as an alternative for food processing industries.