Free-Standing Hydrogel Microarrays : Open-Channel Microfluidics for Massively Parellel Protein Electrophoresis (original) (raw)

We report here for the first time a new open-microchannel architecture for rapid protein electrophoresis. Owing to its open architecture, this technology is optimized for interfacing with automated robotic controllers and downstream processing (e.g., sample spotters, immunological probing, mass spectroscopy). We show the design, fabrication and initial feasibility testing of an open-microchannel array with favorable attributes for up-scaling into high-throughput electrophoretic separations. This multiplexed open-channel platform is uniquely well-suited for massively parallelized proteomics, a major unrealized goal from bioanalytical technology.