Hydrogen bioproduction with anaerobic bacteria consortium from brewery wastewater (original) (raw)
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Research Journal of Microbiology, 2015
The objective of this study was conducted to compare the feasibility of producing hydrogen from food and beverage processing wastewater by anaerobic microflora enriched of starch versus coconut milk sludge at initial pH 6.5 under mesophilic condition (35±2ºC) in a batch reactor. Biohydrogen production could be generated from food and beverage processing wastewater, except winery and brewery wastewater employing the enriching hydrogen-producing bacteria of coconut milk or starch sludge. Results revealed that the maximum cumulative hydrogen production (0.33 L H 2 LG 1 wastewater) was observed from coconut milk wastewater by enriching hydrogen-producing bacteria of coconut milk sludge. It was more than twofold higher than that of enriching hydrogen-producing bacteria of starch sludge (0.15 L H 2 LG 1 wastewater). Composition of volatile fatty acid showed the presence of acetate, butyrate and the lower propionate concentration. Chemical Oxygen Demand (COD) removal was in the range of 4.70-64.98.
Fermentative hydrogen production by microbial consortium
International Journal of …, 2008
Heat pre-treatment of the inoculum associated to the pH control was applied to select hydrogen-producing bacteria and endospores-forming bacteria. The source of inoculum to the heat pre-treatment was from a UASB reactor used in the slaughterhouse waste treatment. The molecular biology analyses indicated that the microbial consortium presented microorganisms affiliated with Enterobacter cloacae (97% and 98%), Clostridium sp. (98%) and Clostridium acetobutyricum (96%), recognized as H 2 and volatile acids' producers. The following assays were carried out in batch reactors in order to verify the efficiencies of sucrose conversion to H 2 by the microbial consortium: (1) 630.0 mg sucrose/L, (2) 1184.0 mg sucrose/L, (3) 1816.0 mg sucrose/L and (4) 4128.0 mg sucrose/L. The subsequent yields were obtained as follows: 15% (1.2 mol H 2 /mol sucrose), 20% (1.6 mol H 2 /mol sucrose), 15% (1.2 mol H 2 /mol sucrose) and 4% (0.3 mol H 2 /mol sucrose), respectively. The intermediary products were acetic acid, butyric acid, methanol and ethanol in all of the anaerobic reactors.
Production Of Hydrogen By Wastewater Sludge Using Anaerobic Fermentation In A Pilot Scale Reactor
2000
The aim of this study is to investigate the production of bio-hydrogen, via dark anaerobic fermentation in a pilot scale reactor under batch conditions, using a mixed microflora. As inoculum, anaerobic digested sludge, collected from municipal wastewater treatment plant, was used after being pre-treated with HCl 1N for 24h (pH 3) in order to inhibit the methanogenic bioactivity. As a source of carbon, sucrose at high concentrations (100 g/l) dissolved in a medium containing salts and micronutrients was employed. During fermentation temperature was maintained at room conditions (20°C), pH ranged from 7.2 to 5.2, due to the formation of Volatile Fatty Acids (VFA s ), that showed a butyrate-type fermentation. The reactor produced approximately 70 l of gas containing hydrogen (46%) and carbon dioxide (54%) with a global yield of 0.32 mol H 2 /mol sucrose, whereas no H 2 S or CH 4 was detected, suggesting that acid pretreatment plays an important role in the elimination of methanogenic bacteria. Formation of VFA s , sucrose concentration and biomass concentration in terms of CFU/ml of Clostridium spp. were reported at different times. The investigation of these parameters led to a kinetic study for the description of the dark anaerobic fermentation process, in order to obtain data for future experiments for the production of biohydrogen with a continuous stirred tank reactor (CSTR).
The hydrogen production by anaerobic bacteria grown on glucose
Four isolates of Clostridium genus were obtained from the activated sludge and one Escherichia coli isolated was found in sheep ruminal fluid. These isolates were identified by microscopic methods and by rRNA sequences. Growth, production of metabolic gases and production of organic acids were measured during the anaerobic cultivation of these isolates with glycerol as sole carbon source. It was found that these isolates from activated sludge were related to Clostridium botulinum, C. perfringens and C. difficile. One strain could not be assigned to any species but was similar to C. botulinum, and one ruminal bacterium was identified as Escherichia coli. All isolates grew on the medium with glycerol as sole carbon source with prolonged lag phase. The lag phase was shorter after adaptation of cells to glycerol. All these strains produced H 2 , and CO in concentration range 10 2 µmol L -1 , and H 2 S in concentrations lower by one order of magnitude. Kinetics of evolution of these gases was different suggesting that they are produced by independent processes. The major organic acid produced with glycerol as the carbon source was acetic acid. Butyric, formic, and propionic acids were produced in dependence on the isolated tested. Results show that metabolic gases are produced mainly in the exponential phase of growth.
Biological hydrogen production by anaerobic sludge at various temperatures
International Journal of Hydrogen Energy, 2006
In this paper, experiments were conducted to investigate H 2 production from glucose by mixed anaerobic cultures at various temperatures in the mesophilic range. Results showed that glucose degradation rate and efficiency, H 2 yield, and growth rate of H 2 -producing bacteria all increased as the temperature increased from 33 to 41 • C. However, the specific H 2 production rate increased with increasing temperature from 33 to 39 • C, then decreased as the temperature was further increased to 41 • C. The distribution of aqueous products was also greatly influenced by temperature variation. H 2 yield and growth rate of H 2producing cultures had a linear relationship with temperature. A modified Gompertz model was able to adequately describe the H 2 production and microbial growth in the mesophilic range. The activation energies for H 2 production and microbial growth were estimated as 107.66 and 204.77 kJ/mol, respectively. ᭧
Hydrogen bioproduction with Enterobacter sp. isolated from brewery wastewater
International Journal of Hydrogen Energy
Hydrogen-producing bacterial strains were isolated from granular sludge from a UASB reactor that treats brewery wastewater. Most of the isolated strains were related to the Enterobacter genus through a phylogenetic analysis of the 16S rRNA sequences. The strains could use various carbon sources (sugars and glycerol) to produce hydrogen. The isolated strain, identified as Enterobacter sp. based on 16S rRNA gene sequencing, produced 6.8 mmol H 2 L À1 culture medium when growing on glucose (2.0 g L À1) in anaerobic conditions at 30 C. The main liquid metabolites were acetic acid (367 mg L À1), methanol (437 mg L À1) and ethanol (1101.26 mg L À1), after 9 h of fermentation. The maximum hydrogen yield of 0.8 mol H 2 /mol glucose was observed, indicating that Enterobacter sp. isolated from brewery wastewater was an efficient hydrogen-producing bacterium under mesophilic conditions.
Bioresource Technology, 2011
A system for biohydrogen production was developed based on long-term continuous cultures grown on sugar beet molasses in packed bed reactors. In two separate cultures, consortia of fermentative bacteria developed as biofilms on granitic stones. In one of the cultures, a granular sludge was also formed. Metagenomic analysis of the microbial communities by 454-pyrosequencing of amplified 16S rDNA fragments revealed that the overall biodiversity of the hydrogen-producing cultures was quite small. The stone biofilm from the culture without granular sludge was dominated by Clostridiaceae and heterolactic fermentation bacteria, mainly Leuconostocaeae. Representatives of the Leuconostocaeae and Enterobacteriaceae were dominant in both the granules and the stone biofilm formed in the granular sludge culture. The culture containing granular sludge produced hydrogen significantly more effectively than that containing only the stone biofilm: 5.43 vs. 2.8 mol H 2 /mol sucrose from molasses, respectively. The speculations that lactic acid bacteria may favor hydrogen production are discussed.
The hydrogen production by anaerobic bacteria grown on glucose and glycerol
2011
Four isolates of Clostridium genus were obtained from the activated sludge and one Escherichia coli isolated was found in sheep rumina l fluid. These isolates were identified by microscopic methods and by rRNA sequences. Growth, production of metabolic gases and production of organic acids were measured during th e anaerobic cultivation of these isolates with glycerol as sole carbon source. It was found t hat these isolates from activated sludge were related to Clostridium botulinum , C. perfringens and C. difficile . One strain could not be assigned to any species but was similar to C. botulinum, and one ruminal bacterium was identified as Escherichia coli. All isolates grew on the medium with glycerol as so le carbon source with prolonged lag phase. The lag phase was shorter after adaptation of cells to glycerol. All these strains produced H 2, and CO in concentration range 10 2 µmol L ‐1 , and H 2S in concentrations lower by one order of magnitude. Kinetics of evolution of these ...
Korean Journal of Chemical Engineering, 2012
Five pretreatment methods, namely chemical, acid, heat-shock, freezing and thawing, and base, were evaluated for the enrichment of hydrogen-producing bacteria in anaerobic granulated sludge, which will be subsequently used as seed in biological hydrogen production. All the pretreatments showed positive effects towards improving hydrogen (H 2) generation by the microbial population with higher hydrogen production yield and COD removal efficiency as compared to control. The granulated sludge pretreated by heat-shock showed maximum accumulated H 2 (19.48mLg -1-COD), COD removal efficiency (62%), and biomass concentration (22.5 gL -1). © 2012 Korean Institute of Chemical Engineers, Seoul, Korea. http://link.springer.com/article/10.1007/s11814-012-0018-z