Determination of galantamine in human plasma by LC-MS/MS using carbamazepine as an internal standard: Method validation and application to a pharmacokinetic study of galantamine hydrobromide prolonged-release capsules in healthy Thai volunteers (original) (raw)

A rapid, sensitive and reliable LC-MS/MS method for the determination of galantamine in plasma was developed and validated for the pharmacokinetic study of galantamine hydrobromide 8 mg prolonged-release capsules. The plasma sample was prepared by simple liquid-liquid extraction with dichloromethane. Chromatographic separation was performed on a Hypurity C4 (150 x 4.6 mm, particle size 5.0 µm) using isocratic acetonitrile: 10 mM ammonium formate (90:10) as a mobile phase at a flow rate of 0.8 mL/ min. Galantamine was detected by mass spectrometry using the electrospray ion source in the selected reaction monitoring mode. Carbamazepine was used as an internal standard (IS). The extraction recovery was 105.45-111.84% for galantamine and 107.35% for IS. Linearity was found within the calibration range of 0.39-62.5 ng/mL. The intra-day and inter-day accuracy were achieved with the mean concentrations of the quality control samples as 91.92-100.97% and 94.29-102.07%, respectively. The intra-ABOUT THE AUTHOR Dr. Darunee Hongwiset completed her doctoral degree from Heinrich-Heine-Universität Düsseldorf, Germany, after receiving M.S. in Pharmaceutical Chemistry from Chulalongkorn University (CU) and B.Pharm. from Chiang Mai University (CMU). Associated Prof. Dr. Songwut Yotsawimonwat completed his B.Pharm., M. S. and Ph.D. from CMU. He is well versed in pharmaceutical technology research. Associated Prof. Chokchai and Assistance Prof. Dr. Chuleegone Sornsuvit finished his and her graduation from Mahidol University in the field of Pharmaceutical Care. Miss Saowarunee Sangsrijan graduated from Faculty of Sciences, CMU. She is an expert in bioanalysis and bioequivalence study. We are staffs in Faculty of Pharmacy, CMU, and also in Pharmacy Service Center. We are interested in pharmaceutical development and bioanalytical study, especially in bioavailability and bioequivalence study.

Sign up for access to the world's latest research.

checkGet notified about relevant papers

checkSave papers to use in your research

checkJoin the discussion with peers

checkTrack your impact