Species Differences and Mosaic Expression in Duodenum of Both Species Intestinal Carbamoyl Phosphate Synthase I in Human and Rat: Expression During Development Shows (original) (raw)

2013

Abstract

SUMMARY The clinical importance of carbamoyl phosphate synthase I (CPSI) relates to itscapacity to metabolize ammonia, because CPSI deficiencies cause lethal serum ammonialevels. Although some metabolic parameters concerning liver and intestinal CPSI have beenreported, the extent to which enterocytes contribute to ammonia conversion remains un-clear without a detailed description of its developmental and spatial expression patterns.Therefore, we determined the patterns of enterocytic CPSI mRNA and protein expression inhuman and rat intestine during embryonic and postnatal development, using in situ hy-bridization and immunohistochemistry. CPSI protein appeared during human embryogene-sis in liver at 31–35 e.d. (embryonic days) before intestine (59 e.d.), whereas in rat CPSI de-tection in intestine (at 16 e.d.) preceded liver (20 e.d.). During all stages of developmentthere was a good correlation between the expression of CPSI protein and mRNA in the in-testinal epithelium. Strikingl...

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