Induction of apoptosis in B-CLL cells by selected histone deacetylase inhibitors (original) (raw)
Histone modifications influence the chromatin structure, changing the patterns of gene expression. Histone deacetylases (HDACs) comprise a group of enzymes governing acetylation status of N-terminal lysine residues of four core histones. Acetylation, the result of histone deacetylases inhibition, leads to cell cycle arrest and differentiation or apoptosis in neoplastic cells. The aim of the study was to induce apoptosis in B-cell chronic lymphocytic leukemia cells in vitro using histone deacetylase inhibitors. B-CLL cells isolated from peripheral blood of 30 patients were examined after 24 hour culture with histone deacetylase inhibitors: phenylbutyric acid and sodium butyrate. Control B-CLL cells were cultured either with dexamethasone (positive control of apoptosis) or media alone (negative control of apoptosis). Normal cells were also examined in this study: 6 specimens of B-lymphocytes isolated from tonsils and 6 specimens of peripheral blood lymphocytes isolated from healthy bl...
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