Synthesis of Libraries and Multi-Site Mutagenesis using a PCR derived, dU-containing Template (original) (raw)
A simple method for site-directed mutagenesis using the polymerase chain reaction
David Galas
Nucleic Acids Research, 1989
View PDFchevron_right
A novel PCR strategy for high-efficiency, automated site-directed mutagenesis
Ping Liu
Nucleic Acids Research, 2005
View PDFchevron_right
PCR-based strategy for construction of multi-site-saturation mutagenic expression library
Sophia Zhang
Journal of Microbiological Methods, 2007
View PDFchevron_right
Multiplex site-directed mutagenesis strategy including high-efficiency selection of the mutant PCR products
Zsolt Nagy
Biotechnology Letters, 2007
View PDFchevron_right
A one-tube, two-step polymerase chain reaction-based site-directed mutagenesis method with simple identification of the mutated product
László Puskás
Analytical Biochemistry, 2004
View PDFchevron_right
A fast and simple method for simultaneous mixed site-specific mutagenesis of a wide coding sequence
Ciro Isidoro
Biotechnology and Applied Biochemistry, 2008
View PDFchevron_right
Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates
Jerome Peyralans
Applied Microbiology and Biotechnology, 2008
View PDFchevron_right
Rapid and efficient site-specific mutagenesis without phenotypic selection
hazem eldebaky
Methods in enzymology, 1987
View PDFchevron_right
Site-Directed Mutagenesis for Large Insertions by Oligonucleotide Primers in Optimized Molar Ratios
H. Sul
BioTechniques, 1997
View PDFchevron_right
Cloning independent site-directed mutagenesis using total RNA as template
Zsolt Nagy
BioTechniques, 2004
View PDFchevron_right
Random mutagenesis libraries: optimization and simplification by PCR
jessica Zucman-Rossi
BioTechniques, 1999
View PDFchevron_right
One-Tube-Only Standardized Site-Directed Mutagenesis: An Alternative Approach to Generate Amino Acid Substitution Collections
Sandra Luna
PloS one, 2016
View PDFchevron_right
An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes
Etienne Joly
Nucleic Acids Research, 1998
View PDFchevron_right
A novel method for site-directed mutagenesis using PCR and uracil DNA glycosylase
Ayoub Rashtchian
Genome Research, 1992
View PDFchevron_right
A rapid and efficient method for multiple-site mutagenesis with a modified overlap extension PCR
Jianfei Ji
Applied Microbiology and Biotechnology, 2005
View PDFchevron_right
A simple and efficient method for site-directed mutagenesis with double-stranded plasmid DNA
Xueli Zhu
Nucleic Acids Research, 1993
View PDFchevron_right
MALS: an efficient strategy for multiple site-directed mutagenesis employing a combination of DNA amplification, ligation and suppression PCR
Dennis Drayna
BMC Biotechnology, 2009
View PDFchevron_right
High-throughput site-directed mutagenesis using oligonucleotides synthesized on DNA chips
Vincent Dugas
Biotechniques, 2005
View PDFchevron_right
dRTP and dPTP a complementary nucleotide couple for the Sequence Saturation Mutagenesis (SeSaM) method
Rajni Verma, Danilo Roccatano
View PDFchevron_right
Multiple Site Directed Mutagenesis Strategy Based on Total RNA and RT-PCR Method
Zsolt Nagy
Molecular Biotechnology, 2007
View PDFchevron_right
Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA
Michael Smith
Nucleic Acids Research, 1982
View PDFchevron_right
Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies
Aaron Hanukoglu
BMC Biotechnology, 2009
View PDFchevron_right
Site-directed mutagenesis of double-stranded DNA by the polymerase chain reaction
Janice Cline
Gene, 1994
View PDFchevron_right
Directed-Mutagenesis and Deletion Generated through an Improved Overlapping-Extension PCR Based Procedure
Frank Robb
Silpakorn U Science & …, 2007
View PDFchevron_right
Improved oligonudeotide site-directed mutagenesis using M13 vectors
Hugues Bedouelle
Nucleic Acids Research, 1985
View PDFchevron_right
Transversion-enriched sequence saturation mutagenesis (SeSaM-Tv+): A random mutagenesis method with consecutive nucleotide exchanges that complements the bias of error-prone PCR
Danilo Roccatano
Biotechnology Journal, 2008
View PDFchevron_right
Site-Directed Mutagenesis Using the Megaprimer Method
Dieter C Gruenert
E. coli Plasmid Vectors, 2003
View PDFchevron_right
Construction of a high-efficiency multi-site-directed mutagenesis
Masaharu Seno
AFRICAN JOURNAL OF BIOTECHNOLOGY
View PDFchevron_right
Site-specific mutagenesis using synthetic oligodeoxyribonucleotide primers: I. Optimum conditions and minimum oligodeoxyribonucleotide length
Michael Smith
Gene, 1979
View PDFchevron_right
SDM-assist software to design site-directed mutagenesis primers introducing “silent” restriction sites
Abhijit Karnik
BMC Bioinformatics, 2013
View PDFchevron_right
Transposon-directed base-exchange mutagenesis (TDEM): a novel method for multiple-nucleotide substitutions within a target gene
Sherie L. Morrison
BioTechniques, 2009
View PDFchevron_right
AAscan, PCRdesign and MutantChecker: a suite of programs for primer design and sequence analysis for high-throughput scanning mutagenesis
Dmitry Veprintsev
PloS one, 2013
View PDFchevron_right