STABILITY INDICATING RP-HPLC METHOD FOR Ceftaroline Fosamil acetate (original) (raw)
Plan: Stability indicating RP-HPLC method for the ceftaroline fosamil acetate. Preface: The developed method is simple, fast and accurate and can be used for routine analysis of Market formulations. Methodology: Stability indicating reverse phase high performance liquid chromatography method was developed and validated for the analysis of ceftaroline fosamil in bulk form. Chromatographic separation was achieved on a X-Bridge shield RP (4.6×100mm, 5µ) column, maintained at 30 0 c with a mobile phase consisting of acetonitrile and ammonium dihydrogen ortho phosphate buffer in the ratio of 10: 90. The mobile phase pumped at a rate of 1 ml/ min. and the detection was carried out at 242.6nm by PDA detector. The retention time was obtained 4.312.The peak area plot was linear over the concentration range of 50µg/ml to 250µg/ml. The different experimental parameters affecting the stability were optimized. The method was validated for accuracy, precision, specificity, robustness, LOD and LOQ in accordance with International Conference on Harmonization (ICH) guidelines. Outcome: The proposed method was successfully applied for the analysis of ceftaroline fosamil in bulk form.
Sign up for access to the world's latest research.
checkGet notified about relevant papers
checkSave papers to use in your research
checkJoin the discussion with peers
checkTrack your impact
Loading Preview
Sorry, preview is currently unavailable. You can download the paper by clicking the button above.