Dye-induced fluorescence of tumour cells: photochemical action spectra (original) (raw)

We have previously reported that granules of tumour cells supravitally stained with thiazine dyes show a fluorescence which changes when exposed to light. This paper reports studies of the action spectra of this reaction. Action spectra of Sarcoma 37 ascitic tumour cells, fixed and living, stained with the oxazine dye Nile Blue, the thiazine dye Azure II, and the monoazo dye Janus Green B, display maxima in the 365–400 nm region. The action spectra peaks do not coincide with the absorption maxima of the dyes in solution. The information given in these action spectra has enabled the effects of fixation to be studied.