Chromosomal Changes in Childhood Acute Lymphoblastic Leukemia in Mosul (original) (raw)
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Objectives: The aim of the present study is to evaluate the frequency of chromosomal abnormalities in childhood acute lymphoblastic leukemia at a tertiary care hospital of Sindh. Study design: Observation study. Place of study: Isra University Hospital, Hyderabad and Oncology Unit Liaquat University of Medical and Health Sciences, Jamshoro. Duration of study: From January 2014 to March 2015. Materials and Methods: Cytogenetic analysis was conducted on peripheral blood and bone marrow samples of 100 diagnosed cases of acute lymphoblastic leukemia (ALL). Peripheral blood and bone marrow samples were collected and putted into sodium heparinized bottles. Cytogenetic analysis was performed by karyotyping according to the ISCN guidelines for human cytogenetic nomenclature using cytovision-+ system for image analysis. Data was analyzed on statistic 8.1 USA and expressed as means, percentage and chi-square with P-value of ≤ 0.05 being defined significant. Results: Chromosomal abnormalities were found in 53% of the ALL cases. Numerical abnormalities were found in 71% whereas 35% cases showed structural abnormalities. 29% cases of ALL showed diploidy and aneuploidy was found in 69% of cases and 2% cases were unknown. Highest number of patients 51% showed hyperploidy followed by 12% cases of hypoploidy and 6% showed pseudoploidy. Chromosomal translocations t(9; 22) (q34; q11) and t(8; 22) (q24; q11) were noted in 6% each and t(8; 14) (q22; q32) were seen in 5% of the cases of childhood ALL. Conclusion: The present study reports chromosomal abnormalities in 53% of cases. Numerical abnormalities were found in 71% whereas 35% cases showed structural abnormalities.
Cytogenetic Profile of Childhood Acute Lymphoblastic Leukemia in Oman
Archives of Medical Research, 2007
Background. Chromosomal abnormalities have important diagnostic and prognostic significance in acute lymphoblastic leukemia (ALL). The purpose of this study was to define and classify the frequency and type of chromosomal abnormalities among newly diagnosed children with ALL and compare the results with those reported from other geographical regions of the world. Methods. Bone marrow chromosomal studies with GTG banding were performed in untreated ALL pediatric patients aged from 7 days to 14 years. Results. Among Omani children examined with ALL, 47 (81%) patients yielded results, with 26 (55.3%) showing an abnormal karyotype [10 (21.3%) pseudodiploid, 2 (4.3%) hypodiploid and 14 (29.7%) hyperdiploidy] and 21 (44.6%) had normal diploidy. Structural abnormalities were observed in 16 (34%), of which 11 (23.4%) cases were translocations, the most frequent being t(9;22) observed in three (6.4%) of our patients. Uncommon translocations such as t(9;15)(p11;q10), t(3;6)(p12;q11), t(1;6)(?31;?q23), t(1;19)(q12;q12), der(18)t(12;18)(q11;p11), and other structural aberrations add(2)(q22), add(6)(q16), add(18)(q22), add(14)(q32) along with deletions del(10)(q22), del(12)(p11), del(12)(p12), del(18)(q11) were also observed. Conclusions. The study showed a good correlation and concordance between the ploidy distribution by cytogenetics and flow cytometry. The patterns of chromosomal anomalies in our patients showed some variations in the frequency of aberrations reported. It is therefore necessary that newer techniques like fluorescence in situ hybridization (FISH) along with reverse transcriptase polymerase chain reaction (RT-PCR) and spectral karyotyping will help us identify chromosomal aberrations not detected by conventional cytogenetic methods in the near future. To our knowledge, this is the first report from the Middle East of a cytogenetic study on childhood ALL. Ó 2007 IMSS. Published by Elsevier Inc.
Journal of the Royal Medical Services, 2015
Objective: To determine the spectrum of cytogenetic abnormalities of acute lymphoblastic leukemia in children and adults at King Hussein Medical Center. Methods: A retrospective review of raw bone marrow aspirate cytogenetic analysis reports was conducted at Princess Iman Research and Laboratory Sciences Center at King Hussein Medical Center during the period between Jan 2010 and Apr 2014. A total of 97 patients were studied regarding: age, gender, and cytogenetic analysis. The age was categorized into two groups (≤14 years as children group and >14 years as adult group). Descriptive analysis using frequencies was used to describe the study variables. Results: Fifty-two (53.6%) cases were males and 45 (46.4%) were females. Their ages ranged between six months and 72 years. A total of 72 (74.2%) patients were children and 25 (25.8%) patients were > 14 years old. Of all pediatric acute lymphoblastic leukemia cases, 52.8% (38 cases) were negative for all the cytogenetic abnormalities, while 47.2% (34 cases) revealed one or more cytogenetic abnormalities. Translocation t(12;21), hyperdiploidy (>50 chromosomes or DNA index >1.16) were the predominant cytogenetic abnormalities in children with lymphoblastic leukemia. In the adult lymphoblastic leukemia group, 68.0% (17 cases) were negative for all the cytogenetic abnormalities, while 32% (8 cases) had cytogenetic abnormalities. Hyperdiploidy was the most common (20.0%, 5 patients) followed by translocation t (9;22) (8.0%, 2 patients). Conclusion: Distribution and patterns of chromosomal abnormalities of lymphoblastic leukemia differ between children and adults. Translocation t(12;21), hyperdiploidy and rearrangements / translocations involving the MLL gene at chromosome 11q23 were the most commonly encountered in children. Hyperdiploidy was prevalent in adults, while no adult cases with 11q23 rearrangements or t(12;21) were encountered.
Clinical Lymphoma Myeloma and Leukemia, 2018
Acute lymphoblastic leukemia (ALL) is the commonest malignancy in children, with a peak incidence of 2 to 3 years, accounting for almost 30% of all cancers in this age group. It is well established that the identification of cytogenetic abnormalities is highly relevant for prognosis and therapeutic decisions in ALL. The purpose of this study is to define the frequency of recurrent chromosomal abnormalities of ALL in Moroccan patients referred exclusively to BIOLAB laboratory from the children hospital of Rabat over a period of 4 years and compare our findings to the literature. In this study, we performed conventional karyotype to 155 ALL cases with a successful cell culture rate of 94%. We identified chromosomal abnormalities in 66% of the total studied cases, of which 70% revealed important recurrent abnormalities with high prognosis values, such as hyperdiploidy, hypodiploidy, t(9;22), t(8;14), t(1;19) and MLL rearrangements. In total coherence with the literature, the majority of patients (56%), in the present study, were in the age group of 1 to 5 years with male predominance, and BALL was the most common blast phenotype (85%). The frequency of most chromosomal rearrangements successfully identified in our study and their lineage belonging was in correlation with the ones published in the literature.
Chromosomal abnormalities in Pakistani children with acute lymphoblastic leukemia
Asian Pacific journal of cancer prevention : APJCP, 2014
Cytogenetic abnormalities have important implications in diagnosis and prognosis of acute leukemia and are now considered an important part of the diagnostic workup at presentation. Karyotype, if known at the time of diagnosis, guides physicians to plan appropriate management strategies for their patients. To determine the cytogenetic profile of acute lymphoblastic leukemia (ALL) in Pakistani children in order to have insights regarding behavior of the disease. A retrospective analysis of all the cases of ALL…
Cytogenetic findings of patients with acute lymphoblastic leukemia in fars province
Iranian journal of medical sciences, 2013
Acute lymphoblastic leukemia (ALL) is the sixth most common malignancy in Iran. Cytogenetic analysis of leukemic blasts plays an important role in classification and prognosis in ALL patients. The purpose of this study was to define the frequency of chromosomal abnormalities of ALL patients in adults and children in Fars province, Iran. In this cross-sectional study, we evaluated karyotype results of bone marrow specimens in 168 Iranian patients with ALL (154 B-ALL and 14 T-ALL) in Fars Province using the conventional cytogenetic G-banding method. The frequency of cytogenetic abnormalities, including numerical and/or structural changes, was 61.7% and 53.8% in the B-ALL and T-ALL patients, respectively. Hyperdiploidy was the most common (32%) cytogenetic abnormality. Among structural abnormalities, the most common was t(9;22) in 11% of the patients. The children showed a higher incidence of hyperdiploidy and lower incidence of t(9;22) than adults (P<0.05). We found a lower inciden...
Prognostic cytogenetic markers in childhood acute lymphoblastic leukemia: Cases from Mansoura, Egypt
Hematology, 2006
Objective. To evaluate children with acute lymphoblastic leukemia (ALL) showing resistance to immediate induction chemotherapy in relation to conventional and advanced cytogenetic analysis. Methods. This work was conducted on 63 ALL children (40 males and 23 females) with age range 4.5 months-16 years (mean = 7.76 years). They included 37 cases attained true remission and 26 complicated by failure of remission, early relapse or death. They were subjected to history, clinical examination and investigations including CBC, BM examination, karyotyping, FISH for translocations and flowcytometry for immunophenotyping and minimal residual disease diagnosis. Results. Cases aged < 5 years; male sex with organomegaly had better remission although statistically insignificant. Initially low HB < 8 gm/dl, high WBCs and platelet counts > 50.000/mm 3 also showed better but non-significant remission rates. Most of the present cases were L 2 with better remission compared to other immunophenotypes. Forty informative karyotypes were subdivided into 15 hypodiploid, 10 pseudodiploid, 8 normal diploid and 7 hyperdiploid cases; the best remission rates were noticed among the most frequent ploidy patterns. Chromosomes 9, 11 and 22 were the most frequently involved by structural aberrations followed by chromosomes 5, 12 and 17. Resistance was noted with aberrations not encountered among remission group; deletions involving chromosomes 2p, 3q, 10p and 12q; translocations involving chromosome 5; trisomies of chromosomes 16 and 21; monosomies of 5 and X and inversions of 5 and 11. Conclusion. Some cytogenetic and molecular characterizations of childhood ALL could add prognostic criteria for proper therapy allocation.
Caryologia, 2011
Pre treatment diagnostic cytogenetics is one of the most important prognostic indicators of pediatric acute lymphoblastic leukemia (ALL).Bone marrow aspirate samples of 31 cases of pediatric ALL were analyzed by routine G-Banding technique. Karyotype analysis was done as per International System for Cytogenetic Nomenclature (ISCN), 2005 criteria. Sixteen out of 31 (51.2%) cases were hypodiploid (2n<46), 10/31(32.0%) hyperdiploid (2n>46) and 5/31(16.0%) aneuploid. Among hypodiploid groups, nine (29.0%) had modal chromosome number as 31-39, fi ve (16.0%) as 40-45 and two (6.5%) as 25-30. Among hyperdiploid group, 07(22.5%) had modal chromosome number as 51-60 followed by 2n=47-50 (three cases, 6.5%). The chromosomes (Chr.) 2, 10, 12, 15, 17, 19 were commonly deleted in hypodiploid cell lines whereas gain of Chr. 4, 8, 10, 14and 20 were observed in hyperdiploid group. Translocations t(10;14),t(9;22),t(2;22),t(8;22) andt(4;11)were seen in 04(12.8%),03(9.6%),and02(6.4%each) and one case respectively. To conclude a high proportion of cases in this study showed adverse cytogenetic parameters such as hypodiplody and translocations such as t(10;14),t(9;22),t(2;22),t(8;22)andt(4;11).