Histopathological and Ultrastructure Features of Hydatid Cyst from Slaughtered Animals in Egypt (original) (raw)
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Histopathological Study Of The Fibrotic Layer Of Hydatid Cyst In Camel, Cow And Sheep Research
Journal of Clinical Cases, 2023
Hydatidosis or echinococcosis is one of the most important common diseases between humans and animals. 1.1. Aim: Hydatid cyst is often caused by Echinococcus granulosus worm. The closest host tissue to the cyst is the fibrous layer. The purpose of this study is to investigate any differences in the fibrosis layer in the lung and liver of ruminants. 1.2. Methods: For each of these three animals, 22 infected lung or liver samples were collected from Tehran slaughterhouses during the months of May to July 2022. A piece of one square centimeter was removed from the fibrotic layer and also from the healthy tissue near the cyst. In the usual process of section preparation, pathological observation was included. Sections were observed and reported by a pathologist. 1.3. Findings: In total, 138 sections were studied pathologically. The diameter of the cysts varied between 1.5-0.6 cm in camels and cattle and 0.5-0.2 cm in sheep. Most cases of sterile cysts were seen in cows. Fibrous layer was present in all (100%) of the 66 studied samples. Necrosis was observed in 20 cases (30.30%), including 12 cases in the lungs of camels, 6 cases in the lungs of cows, and only 2 cases, one in the lung and the other in the liver of sheep. Only one case (1.52%) of granuloma was observed in cow liver. 1.4. Conclusion: Differences were observed in the fibrotic layer of hydatid cyst in cows, camels and sheep. In all 3 types of animals, the rate of progression of the lesion was towards the formation of necrosis and granuloma, and also, the frequency of these lesions had a significant difference, which is due to the difference in the parasite strain, the host type, and also the average age of these animals
Experiment of Hydatid Cyst in Two Strains (Camels and Goats) in Saudi Arabia
Open Journal of Animal Sciences, 2019
Hydatid cyst disease is one of the most parasitic diseases transmitted from carnivores animals (such as dogs) to humans and causes a deterioration in the general health. Its transmission to herbivores animals led to a substantial economic loss in the meat productivity and reduced its quality. The increase of acquisition of dogs at homes nowadays led to an increase in the risk of infection with many parasitic diseases such as hydatid cyst disease. The present study was conducted to provide a modern view on the current status of hydatid cyst disease among slaughtered animals in Jeddah through periodic visiting to a slaughter house in North Jeddah. The mice prepared have been infected in the peritoneal membrane with 0.2 ml of hydatid cyst fluid containing ±2000 protoscoleces from fertile cysts obtained from an infected goat's liver which resulted in a 75% infection rate. Mice infected with hydatid fluid from fertile cysts obtained from an infected camel's lungs showed a result of 11.1% infection rate. The cysts appeared in the peritoneal and abdominal cavity.
CHARACTERISTICS OF HYDATID CYST OF CAMEL STRAIN OF ECHINOCOCCUS GRANULOSUS IN ASSIUT
minia.edu.eg
100 camels were examined for hydatid cyst and revealed that the prevalence rate of cystic hydatidosis among camels in Assiut Governorate was 9% .The lung was the most suitable place for cystic infection in camel than the liver. The fertility rate of the examined cysts was 80%. Hydatid cyst wall was better stained with PAS. Best carmine and Giemsa stains were very rapid effective methods for visualization of hydatid elements. Scanning electron microscope was used for the detection of ultrastructure features of hydatid sand. It revealed that camel strain has some specific characters including large sized rostellar hooks and presence of separation line between the scolex and the neck in the evaginated protoscolices. In top view the rostellum appeared to be formed of 3 successive concentric rings becoming smaller as they go upwards. This was shown for the first time in the present study. SDS PAGE was used for characterization of hydatid cyst fluid, protoscolices and laminated layer antigens separately. It revealed that protoscolices and laminated layer are the source of proteins in the hydatid fluid. Many protein bands ranged from 150-6 kD were detected by different stains. The pattern of these bands indicated that camel's hydatid cyst components differ from that of sheep and cattle.
Histopathological study of hepatic and pulmonary sheep's hydatidosis
Ann Trop Med & Public Health, 2019
Echinococcosis or hydatid disease is caused by infection with cestoda tapeworm belonging to the genus Echinococcus. It caused public health and economic problems worldwide. Different species responsible for this disease include E. granulosus, E. multilocularis and E. vogeli , etc. Most sites of infection are liver and lung with slow development of the cyst (larval stage), causing many pathological effects that occur in these organs. This study was aimed to investigate different histopathological changes in the liver and lung of sheep's infected with hydatid cysts. Many histopathological variations were noticed in the liver that infected with E. granulosus cysts included hepatocytes degeneration , nuclear changes , necrosis and a steatosis on infected liver sections. Fatty changes also noticed. Increasing in kupffer cells with participation recorded in most sections. Lung sections showed extensive tissue reaction accompanied with inflammatory cells infiltrate around the necrotic areas. Sections also revealed formation of granuloma compact fibrous structure of hydatid cysts and fibrin rich exudate cause hyaline formation.
Echinococcosis, a worldwide zoonosis, is caused by the larval stages of Echinococcus granulosus and E. multilocularis belonging to the family taeniidae. Because of the increasing consumption of red meat; survey, prevention and treatment of this disease will be essential. Also detection of contaminant factors including parasitic agents and the factors that are related to the hosts is helpful to codify a useful programme for prevention of this disease. This slaughterhouse based survey has been done to determine the rate of hydatidosis in Ilam province on 2380 sheep, 975 goats and 1820 cows. Overall 10% of infected livers and lungs from each animal group randomly collected and the number of cysts was determined macroscopically. The rate of hydatidosis was 6.8 % in sheep, 4.2 % in goats and 8.6 % in cows. The infection rates of lungs in sheep, goats and cows were 82, 73 and 75%, respectively, while the infection rates of livers were 12, 8 and 13%, in sheep, goats and cows, respectively. The difference of infection rates between lung and liver was statistically significant (p<0.05). After transferring of specimens to the laboratory for detection of protoscolices, results shows that the rate of alveolar cysts were teemed in sheep, cows and goats were 12 %, 24 % and 22 %, respectively and also exuberated hepatic cysts in sheep, cows and goats were 25 %, 2.5 % and 18 %, respectively. The rates of alive protoscolices in fertile cysts were determined by eosin staining and the percentage of protoscolices that were stained were considered alive. Their cumulative rates were 79.45, 88.95 and 72.02 % while their rates in livers were 65.71, 38.45 and 86.14% in sheep, cows and goats, respectively. These results showed a considerable infection of sheep and goats in this region. It could be concluded that because sheep, goats and guard dogs are maintained close to together, therefore these high rates of infection are due to continuous contact of these animals with dogs and their feces. Also low knowledge of people about true prevention strategies especially in the deprived region that has nomadic inhabitants may be the main cause of increasing of hydatid cyst infestation. The prevention strategies like suitable annihilation of infected viscera would be key task in decreasing hydatid cyst rate in Ilam.
Histopathological study of hepatic and pulmonary sheep__ampersandsign#39;s hydatidosis
2019
Echinococcosis or hydatid disease is caused by infection with cestoda tapeworm belonging to the genus Echinococcus. It caused public health and economic problems worldwide. Different species responsible for this disease include E. granulosus, E. multilocularis and E. vogeli , etc. Most sites of infection are liver and lung with slow development of the cyst (larval stage), causing many pathological effects that occur in these organs. This study was aimed to investigate different histopathological changes in the liver and lung of sheep's infected with hydatid cysts. Many histopathological variations were noticed in the liver that infected with E. granulosus cysts included hepatocytes degeneration , nuclear changes , necrosis and a steatosis on infected liver sections. Fatty changes also noticed. Increasing in kupffer cells with participation recorded in most sections. Lung sections showed extensive tissue reaction accompanied with inflammatory cells infiltrate around the necrotic areas. Sections also revealed formation of granuloma compact fibrous structure of hydatid cysts and fibrin rich exudate cause hyaline formation.
A histopathological study of hydatid cyst in donkey at Basrah city, Iraq
Hydatid disease is a cosmopolitan , An incident of case of donkey found after anatomy with pulmonary hydatid cyst infection which showed after make a pathological sections a many changes, like thick fibrous capsule, remnant of hydatid cyst with calcification and fibrosis, other fibrous capsule at the periphery capsule with infiltration of inflammatory cells. A fibrous capsule associated with inflammatory cells in peri and intra capsule were noticed in, other fibrous capsule with calcification of degenerate hydatid cyst. A marked fibrous capsule with mineralization of degenerate hydatid cyst. The lung with many changes, like, a bronchi with hyperplasia and hypertrophy of bronchial epithelial with dilated alveoli, some with area of congestion. A minimal bronchiolites with hypertrophy and hyperplasia of bronchiolar epithelium and congestion, other with area of pulmonary fibrosis with mineralization, a pulmonary fibrosis with few and massive inflammatory cells. In conclusion the hydatid cyst showed in donkey indicating more resistant response than that by sheep and cattle.
Journal of Helminthology, 2007
In a cross-sectional study, a total of 5381 slaughtered animals, namely 928 cattle, 243 buffaloes, 3765 sheep and 445 goats were inspected macroscopically for hydatid cysts in northwest Iran, with prevalence values of 38.3%, 11.9%, 74.4% and 20%, respectively, being recorded. Prevalences were higher in females compared with males, but a significant difference (P < 0.001) was only found in sheep and cattle. Most cases which were condemned were seen in the lungs of sheep (13.4%) indicating that sheep are the most important intermediate hosts for Echinococcus granulosus in this area.
International Journal for Agro Veterinary and Medical Sciences, 2014
A total of 987 carcasses of sheep (459) and goats (528) of different ages, in the period of June, 2012-June, 2013, reared in different localities at El-Kharga city (in and out the abattoir), where 37 (8.06%) of sheep and 29 (5.49%) of goats were found harbored hydatid cyst liver, and lung were the most infected organ in sheep at rate of 4.57% and 2.61%, respectively, but in goats the lung was the most predilection site than liver in rate of 3.03% and 2.46% respectively, both liver and lung were cystic, in sheep only in rate of 0.65%, and the under develop cyst recorded in kidney of sheep 0.28%. Sheep fell in age category of 3 year and above were more infected (15.29%) compared to 1 to 3 year (4.23%) and below 1 year (0%) old. Likewise, the prevalence in goats was 7.89%, 3.79% and 0% respectively in the same age groups. Fertility of cyst was highest in sheep than goats in rate of 62.16% and 58.62%, respectively. The biochemical analysis revealed significant increase in protein level of lung cysts extract (P < 0.01) than liver cyst extract in sheep and goats. While liver cyst extract revealed significant increase in both of, creatinine (P < 0.01), calcium ions (P < 0.01) and magnesium ions (P < 0.05) than lung cyst extract in both sheep and goats, while cholesterol and triglyceride revealed significant increase in liver cyst extract of sheep and no change in liver cyst extract of goats. The differences in chemical component values of cyst extract in both sheep and goat and cystic organ in the same animal may pointed some strain variability in parasite metabolism, growth rate or even strain variation.
Comparative Study Between Serological Analysis and Visual Meat Inspection for Hydatid Cyst in Camels
2014
Received at: 28/1/2014 Accepted: 24/2/2014 The present investigation was conducted (during PM examination of the slaughtered carcasses) to evaluate the sensitivity and specificity of Enzyme-linked immunosorbent assay (ELISA) in identifying camels infected with hydatid cysts before slaughtering and to assess the prevalence of hydatidosis in camels sacrificed in Assuit Governorate. Hydatid cyst count and characterization were conducted based on routine meat inspection. Slaughterhouse samples of 200 camels were collected through weekly visits. Hydatid cysts in livers, lungs and kidneys were detected and counted, also the fertility rate of the cysts was examined. Out of these, 12 (6%) were found to harbor hydatid cyst, in livers 9(75%), lungs 2(17%) and kidney 1(8%).On the other side, fertile cysts 5(41.7%) were found more frequently in livers 4(33.3%) than in lungs 1(8.3%), while sterile cysts7(58.3%) found in livers , lungs and kidneys 5(41.7% ), 1(8.1) and 1(8.3%) respectively. In ad...