Genetic diversity of common bean accessions in the germplasm bank of the Instituto Agronômico–IAC (original) (raw)
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Acta Scientiarum. Agronomy
The present study aimed to analyze, through 12 morpho-agronomic traits and 18 micro satellite loci, the genetic diversity in 17 common bean accessions from the Bean Germplasm Bank of the Center for Applied Agricultural Research of the State University of Maringá (BGF/Nupagri/UEM), in Paraná State, Brazil. Genetic diversity was assessed by joint analysis of phenotypic and genotypic characteristics using the Genetics platform of SAS software. To that end, a dissimilarity matrix was constructed based on the Jaccard index. This was used to generate a dendrogram via UPGMA hierarchical clustering, validated by multidimensional scaling and nonorthogonal principal components analysis. Based on genetic diversity analysis, the accessions were clustered into two large groups: one consisting of 11 accessions of Andean origin and the other containing six Mesoamerican accessions. The 17 accessions from the BGF/Nupagri/UEM were found to be an important source of genetic variability for inclusion in common bean breeding programs, contributing to the development of cultivars with desirable agronomic characteristics.
Genetic divergence in the common bean (Phaseolus vulgaris L.) in the Cerrado-Pantanal ecotone
Genetics and molecular research : GMR, 2017
Evaluating genetic diversity among genotypes is important for providing parameters for the identification of superior genotypes, because the choice of parents that form segregating populations is crucial. Our objectives were to i) evaluate agronomic performance; ii) compare clustering methods; iii) ascertain the relative contributions of the variables evaluated; and iv) identify the most promising hybrids to produce superior segregating populations. The trial was conducted in 2015 at the State University of Mato Grosso do Sul, Brazil. We used a randomized block design with three replications, and recorded the days to emergence, days to flowering, days to maturity, plant height, number of branches, number of pods, number of seeds per pod, weight of 100 grains, and productivity. The genetic diversity of the genotypes was determined by cluster analysis using two dissimilarity measures: the Euclidean distance and the standardized mean Mahalanobis distance using the Ward hierarchical met...
Genetic diversity in common bean accessions evaluated by means of morpho-agronomical and RAPD data
Scientia Agricola, 2007
Germplasm banks store genotype samples, improved varieties, landraces and wild species, all generically denominated accessions. The importance of characterizing germplasm banks is based on the identification and knowledge of relevant traits for genetic improvement and ex situ germplasm conservation. Thus, the present study had as aim the evaluation of the genetic diversity among 220 accessions of a Brazilian common bean germplasm bank of the "Instituto Agronômico de Campinas" (IAC) by means of 23 morpho-agronomical descriptors and 19 RAPD loci. These accessions correspond to genotypes from the Andean and Middle American gene pool as well as from cultivars derived from common bean improvement programs. The Middle American accessions and the improved cultivars were clustered into one group, distinct from the one formed by the Andean accessions. In relation to the molecular data, 47% of the genetic similarity was detected among the Middle American accessions, and similar results were observed for the improved cultivars (50%). The Andean accessions revealed 60% of genetic similarity. The cluster constituted by the improved cultivars and the Middle American genotypes differed, basically, in tegument color. Both molecular and morpho-agronomical data sets were equally effective to quantify and organize the genetic diversity of common bean accessions. This information may be useful to direct crosses and for the proper organization of the IAC germplasm bank.
Genetic divergence in common bean landrace cultivars from Mato Grosso do Sul State
Semina: Ciências Agrárias, 2009
The present study had as objective to evaluate of the genetic divergence among 35 landraces of common bean. The experimental design was carried out in a randomized block with four repetitions. The variance analysis revealed significant difference at 1 and 5% levels of probability for the evaluated characteristics. The Cluster analysis based in the morpho-agronomic traits presented difference among Andean and Mesoamerican landrace cultivars, which it allocated the landrace cultivars in different groups. The Tocher and the Nearest Neighbor methods demonstrated that the most dissimilars cultivars were Carioca sem Cipó and Jalo, whereas Roxinho Mineiro and Carioca sem Cipó cultivars were the more similars. The first two canonic variables were sufficient to explain about 81.90% of the total variation showed in this study. These results reveal large genetic diversity among the landraces from Mato Grosso do Sul state.
Genetics and Molecular Biology, 2006
We used agromorphological and random amplified polymorphic DNA (RAPD) molecular marker data to identify duplicate common bean (Phaseolus vulgaris L. Fabaceae) accessions in the Common Bean Germplasm Bank of the Agronomical Institute - IAC (Banco de Germoplasma de feijoeiro do Instituto Agronômico de Campinas (IAC), SP, Brazil). A total of 116 accessions with the same names and similar agromorphological traits was analyzed. The divergence between the accessions was initially evaluated by means of the agromorphological descriptors using single linkage clustering, from the Euclidean distance. Multivariate analysis identified four duplicate accessions (Carioca Lustroso, Bico de Ouro, Jamapa and Preto), with 17 other same-name accessions being suspected duplicates due to their low divergence levels. Accessions with low genetic distance values (indicating that they were duplicates) were further compared using RAPD markers which confirmed the results of the multivariate analyses in relation to the four duplicate accessions, although only two of the other 17 suspect accessions were confirmed to be duplicates, in this case of accessions IAPAR 57 and Sacavem. These results show that the combined use of agromorphological and molecular information allowed a better characterization of the acessions in the common bean Germplasm Bank.
Seventy four French bean germplasm were evaluated for twenty two quantitative traits to study the genetic diversity during Jan-Feb in G.B. Pant University of Agriculture and Technology. Principal component analysis showed that the first principal component had maximum of 30.85% variation of total variation, while the first seven principal component axes together with explained 83.41% variation, suggesting first seven principal axes are adequate to explain the variation in reduced dimension. Clustering through D 2 analysis revealed maximum inter-cluster distance of clusters V and IX (1405.141) followed by cluster III and IX (1297.298).
Molecular Diversity of Common Bean (Phaseolus Vulgaris L.) Cultivars
2011
RAPD markers were used to evaluate genetic diversity among 8 common bean cultivars (Phaseolus vulgaris L.) that included six developed in Romania and two in CIAT Colombia. Only 4 of all the 8 random primers used in RAPD reactions showed polymorphism acceptable for an effective characterization of bean cultivars. These four primers (OPD-08, OPG-03, OPG-12, OPY-20) generated 33 DNA bands, of which 17 bands showing polymorphism (48.84%). On average, each primer generated 8.25 bands, of which 4.25 were polymorphic. Genetic distances were calculated using Nei&Li (1979) similarity coefficient, displayed in a dendrogram (UPGMA method).Cluster analysis based on RAPD amplification products divided genotypes in two main groups, according to their geographical origin. Genetic similarity vary greatly (60% - 96%), depending on the pairs of genotypes and on the groups.
Qualitative and quantitative descriptors for quantifying the genetic diversity of bean seeds
Trends in Agricultural and Environmental Sciences (TAES), 2023
Beans are one of the main vegetable protein sources for human consumption. Breeders always seek a diversity of bean genotypes to establish and select the best genotypes that promote the greatest genetic gain for the crop. This study aimed to determine the genetic diversity of bean genotypes based on the characterization of qualitative and quantitative descriptors. Samples of 17 bean cultivars were acquired from the local seed market in Cassilândia, MS, Brazil. Four quantitative and eight qualitative descriptors were used. The data were subjected to analysis of variance using a completely randomized design with three replications. Quantitative data means were grouped using the Scott-Knott test at a 5% probability level. Principal component analysis (PCA) was used for the qualitative and quantitative data. Genetic diversity was reported for all quantitative and qualitative traits of bean seeds. The weight of 1,000 seeds (169 to 629 g), seed width (4.8 to 8.0 mm), seed length (8 to 17 mm), and seed thickness (3.7 to 7.4 mm) showed highly significant differences (P<0.001) between the genotypes. Seed color (2 classes), primary color (3 classes) and secondary color (3 classes), seed shape (5 classes), degree of seed flattening (3 classes), seed brightness (3 classes), halo (2 classes) and halo color (2 classes) were the qualitative descriptors measured. The qualitative and quantitative descriptors used in this study proved important for describing the germplasm of the bean crop. The principal component analysis showed that the first two components retain 58% of the data variability, forming five divergent groups. The characterization of the bean genotypes showed genetic diversity in their seeds, and the qualitative and quantitative descriptors used contributed to the adequate separation of the genotypes.