Molecular Characterization of Biodegrading Bacteria from Soil Sample (original) (raw)
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Isolation and Characterization of Bio-Degrading Bacteria from Soil Samples
Biosciences Biotechnology Research Asia, 2011
In the present study, a total of four species of biodegrading bacteria namely Bacillus subtilis, Eschericia coli, Staphylococcus aereus and Pseudomonas putida were isolated from soil samples collected from the sites where lead and mercury were discharged. The effect of various physicochemical parameters on growth of these bacteria was also studied.
Saudi Journal of Biological Sciences, 2020
Toxic metal contamination in soils due industrialization is nowadays a concern to the scientists worldwide. The current study deals with the evaluation of response and tolerance by isolated metallophilic bacteria in different lead concentrations (100 ppm to 1000 ppm). By taking optical densities of the isolates, the minimum inhibitory concentration (MIC) of Pb 2+ were determined.16S rRNA and MALDI-TOF MS were used for the identification of the bacteria. Total of 37 isolates were observed, among them 04 (Staphylococcus equorum, Staphylococcus warneri, Bacillus safensis and Bacillus thuringiensis), isolated were detected having efficacy of Pb 2+ tolerance and sequestration at varying MIC. Furthermore, B. thuringiensis was observed to have highest (900 ppm) tolerance for lead and lowest (500 ppm) for Staphylococcus warneri. Moreover, the highest (65.3%) sequestration potential has been observed for B. thuringiensis and least (52.8%) for S. warneri. The tolerance and sequestration potential properties of these isolated species can be utilised to exterminate heavy metals and reduce their toxicity from the contaminated environment.
Journal of Pure and Applied Microbiology, 2017
The present study deals with isolation and characterization of lead resistant bacteria isolated from two water bodies Udaisagar lake and Gadwa pond of Berach river system, Udaipur, Rajasthan, India. Initially, among 13 of the total isolates screened from water samples, 2 isolates were selected for study based on high level of heavy metal resistances. On the basis of morphological, biochemical and molecular characterization using 16S rDNA sequencing the isolates were identified as Pseudomonas stutzeri (KX692284) and Pseudomonas stutzeri (KX692285). The isolates exhibited high resistance to lead (Pb). The Minimum Inhibitory concentration (MIC) of the isolates against lead was determined using agar plate dilution method. Pseudomonas stutzeri showed highest MIC value for lead up to 1300 mg/l concentration. The uptake of heavy metals, present in water and detoxification of metal ions by bacteria provide an additional mechanism of environmental bioremediation. The identified lead resistan...
Microbial Biomass and Activity in Lead-Contaminated Soil
Applied and Environmental Microbiology, 1999
Microbial community diversity, potential microbial activity, and metal resistance were determined in three soils whose lead contents ranged from 0.00039 to 48 mmol of Pb kg of soil ؊1 . Biomass levels were directly related to lead content. A molecular analysis of 16S rRNAs suggested that each soil contained a complex, diverse microbial community. A statistical analysis of the phospholipid fatty acids indicated that the community in the soil having the highest lead content was not related to the communities in the other soils. All of the soils contained active microbial populations that mineralized [ 14 C]glucose. In all samples, 10 to 15% of the total culturable bacteria were Pb resistant and had MIC of Pb for growth of 100 to 150 M.
Folia Microbiologica Springer, 2020
Electronic waste is an evolving source of harmful pollutants in our surrounding environments and considered to be perilous as it contains toxic metals such as chromium, cadmium, lead, mercury, zinc, and nickel in huge quantities. Heavy metals are harmful contaminants and accumulated in the environment due to various anthropogenic activities. The present study was conducted to isolate and characterize different heavy metal tolerant bacterial species, based on molecular techniques from soil contaminated by electronic waste. The contaminated soil samples were analyzed for various physicochemical properties such as pH, electrical conductivity, soil moisture, water holding capacity, organic carbon, organic matter, available phosphorus, total nitrogen, and potassium using standard procedures. The soil samples were found to contain a higher amount of different heavy metals such as copper, chromium, lead, iron, cadmium, and nickel. Serial dilution and spread plate techniques have been used for bacterial isolation. The identification and molecular characterization of isolated bacterial species were done by biochemical tests and 16S rRNA gene sequencing technique. The 16S rRNA sequencing analysis confirmed the presence of different bacterial species as, Micrococcus aloeverae, Kocuria turfanensis, Bacillus licheniformis, Bacillus jeotgali, Bacillus velezensis, and Bacillus haikouensis. The findings indicated that the e-waste dumping sites are the storehouse of elite bacterial species. The present research study offers a platform for systematic analysis of e-waste sites by microbial profiling that may help in the innovation of novel microorganisms of scientific importance and better biotechnological potential.
Pseudomonas: A Major Bacteria in Heavy Metal Contaminated Soil of South-West Punjab, India
International Journal of Plant and Environment, 2019
Soil microflora is continuously changing with altered soil conditions. These soil alterations are a consequence of heavy metals entering and affecting every sphere of life. Heavy metals are not only hazardous for crops but also affect the soil microbial community. Soil bacteria with the potential of plant growth promotion and multiple metal resistances can be an instrument for crop improvement and heavy metal detoxification. In this study, predominant bacterial community associated with the heavy metal contaminated soil was studied using 16S rRNA gene sequencing in association with culture-based techniques. Elemental metal analysis of collected soil samples showed an elevated level of metal content in the soil. 16S rRNA gene analysis and phylogenetic analysis of 126 bacterial clones revealed the probable predominance of Pseudomonas (40.48%) followed by Flavisolibacter (13.49%). Based on morphological and biochemical characterization, nine Pseudomonas strains were selected from the s...
European Journal of Experimental Biology, 2016
Electronic waste or E-waste refers to electronic devices which are disposed into soil without being processed. These electronic goods are made of plenty of components which may contain toxic substances like heavy metals. Lead, one of the heavy metals present in many electronic goods gets accumulated in the soil and leaches into the surrounding environment if the goods are not processed properly. The metal is toxic at very low concentrations and may damage the flora and fauna. So the present study focuses on isolation and identification of bacteria from the samples collected from different E-waste dump yards located in Hyderabad, India. Based on cultural characteristics, biochemical tests and the ability of the organisms to adsorb lead present in the soil contaminated with the metal, the organisms were identified as Bacillus licheniformis, Bacillus subtilis and Bacillus badius 16SrRNA sequencing studies confirmed the identified strain as Bacillus licheniformis with an accession no. CP000002.3.
Evaluating the Potential of Microorganisms in Bioremediation of Lead Polluted Soil
2020
This study evaluated and compared the abilities of indigenous Bacillus sp. and Micrococcus sp. for treating lead polluted soil. The organisms were inoculated into the contaminated soil samples prepared with the optimum values of screened determinant factors and experimented on 8, 16, 24, 32, 40, 48, and 56 days respectively for lead ion residual concentration using Atomic Absorption Spectrophotometer. Bacillus sp. showed greater remediation strength than Micrococcus sp. The results show that Bacillus sp. attenuated the Pb initial concentration from 150.74 mg/kg to control level in 40 days while Micrococcus sp. achieved that in 48 days. The 56th day residual concentrations and removal efficiencies were 81.43 mg/kg and 46% for Bacillus sp., and 86.91 mg/kg and 42.34% for Micrococcus sp. The removal rate was higher for Bacillus sp. (-0.0715 d-1) than for Micrococcus sp. (-0.0744d-1); and the remediation process was transport controlled. This information will be useful for remediation ...
PLOS ONE, 2023
Pollution by lead (Pb) is an environmental and health threat due to the severity of its toxicity. Microbial bioremediation is an eco-friendly technique used to remediate contaminated soils. This present study was used to evaluate the effect of two bacterial strains isolated and identified from Bizerte lagoon: Cupriavidus metallidurans LBJ (C. metallidurans LBJ) and Pseudomonas stutzeri LBR (P. stutzeri LBR) on the rate of depollution of soil contaminated with Pb from Tunisia. To determine this effect, sterile and non-sterile soil was bioaugmented by P. stutzeri LBR and C. metallidurans LBJ strains individually and in a mixture for 25 days at 30˚C. Results showed that the bioaugmentation of the non-sterile soil by the mixture of P. stutzeri LBR and C. metallidurans LBJ strains gave the best rate of reduction of Pb of 71.02%, compared to a rate of 58.07% and 46.47% respectively for bioaugmentation by the bacterial strains individually. In the case of the sterile soil, results showed that the reduction rate of lead was in the order of 66.96% in the case of the mixture of the two bacterial strains compared with 55.66% and 41.86% respectively for the addition of the two strains individually. These results are confirmed by analysis of the leachate from the sterile and non-sterile soil which showed an increase in the mobility and bioavailability of Pb in soil. These promising results offer another perspective for a soil bioremediation bioprocess applying bacterial bioremediation.
2 3 16S rDNA Pyrosequencing Analysis of Bacterial Community in Heavy Metals Polluted Soils
Soil contamination with heavy metals is a widespread problem, especially prominent on grounds lying in the vicinity of mines, smelters, and other industrial facilities. Many such areas are located in Southern Poland; they are polluted mainly with Pb, Zn, Cd, or Cu, and locally also with Cr. As for now, little is known about most bacterial species thriving in such soils and even less about a core bacterial community-a set of taxa common to polluted soils. Therefore, we wanted to answer the question if such a set could be found in samples differing physicochemically and phytosociologically. To answer the question, we analyzed bacterial communities in three soil samples contaminated with Pb and Zn and two contaminated with Cr and lower levels of Pb and Zn. The communities were assessed with 16S rRNA gene fragments pyrosequencing. It was found that the samples differed significantly and Zn decreased both diversity and species richness at species and family levels, while plant species richness did not correlate with bacterial diversity. In spite of the differences between the samples, they shared many operational taxonomic units (OTUs) and it was possible to delineate the core microbiome of our sample set. The core set of OTUs comprised members of such taxa as Sphingomonas, Candidatus Solibacter, or Flexibacter showing that particular genera might be shared among sites~40 km distant.