Placental histomorphology and morphometry in the pregnant mice treated with cell phone radiation (original) (raw)
Related papers
The prenatal effects of mobile microwave radiation on mice
Medical Research Journal, 2011
The aim of this study was to investigate the histopathological and ultrastructural prenatal effects of mobile phone microwave radiation on mice liver. Materials and methods Pregnant mice were divided into three groups of 10 animals each. Group one (G1) was the control group with mice unexposed to mobile phone. Group two (G2) mice were exposed for half-hour every day during the gestational period with the cell phone in answering state. Group three (G3) mice were exposed during the gestational period for 1 h every day with the cell phone in answering state and for 12 h while the cell phone was in standby state. The microwave radiation produced by mobile phones operated at a microwave frequency range of 900-1800 MHz. Mice fetuses were examined and then killed with an overdose of ether. Liver biopsies were prepared for histopathological and ultrastructural examinations. Results The mean body weight and length of fetuses of G3 showed a statistically significant decrease compared with G1 (P < 0.05). Congestion of the central vein and dilation of hepatic blood sinusoids were the most frequent histopathological findings. The ultrastructural results were apparent in G3 in the form of dilated rough endoplasmic reticulum, loss of mitochondrial cristae and pleomorphic mitochondria, mitochondrial swelling and fragmentation, hepatocytic vaculations, nuclear inclusions, and inflammatory cell infiltration in the periportal areas, of mainly lymphocytes. The results of this study showed a significant reduction in the average body weight and length of fetuses of G2 and G3. Conclusion The ultrastructural damage observed in the examined specimens shows the hepatocellular injuries and consequent systemic impairment of body functions.
Survival Assessment of Mouse Preimplantation Embryos After Exposure to Cell Phone Radiation
Journal of reproduction & infertility
Using cellular phone has rapidly increased all over the world. Also, the concern on the possible health hazards of electromagnetic fields (EMF) induced from cell phones to reproduction has been growing in many countries. The aim of this study was to assess the consequences and effects of exposure to the cell phone radiation on the quality and survival rates of preimplantation embryos in mice. A total of 40 mice (20 females and 20 males), 6 weeks old and sexually mature BALB/c, were used for control and experimental groups. The ovary burses were removed and the zygotes were dissected in the morning after mating. Next, 2-cell embryos were divided into two groups of control (n=150) and experimental (n=150). EMF (900-1800 MHz) was used for four days in experimental group for 30 min/day in culture at 37°C in a CO 2 incubator. The quality of embryos was recorded daily and the fluorescent staining was used for identification of viable blastocysts. All data were compared by Student's t-...
Cranial and postcranial skeletal variations induced in mouse embryos by mobile phone radiation
Pathophysiology, 2010
This study focuses on foetal development following mild daily exposure of pregnant mice to near field electromagnetic radiation emitted by a mobile phone. The investigation was motivated by the fact that the potentially hazardous electromagnetic radiation emitted by mobile phones is currently of tremendous public interest. Physically comparable pregnant mice were exposed to radiofrequency radiation GSM 900 MHz emitted by a mobile phone. Within 5 h after birth most cubs were fixed followed by double staining in toto, and conventional paraffin histology. Other cubs remained with their mothers until teeth eruption. Structural development was assessed by examining newborns for the presence of anomalies and/or variations in soft tissues and skeletal anatomy. Electromagnetic radiofrequency exposed newborns, externally examined, displayed a normal phenotype. Histochemical and histological studies, however, revealed variations in the exposed foetuses with respect to control ones concerning the ossification of cranial bones and thoracic cage ribs, as well as displacement of Meckelian cartilage. Littermates examined after teeth eruption displayed normal phenotypes. It is concluded that mild exposure to mobile phone radiation may affect, although transiently, mouse foetal development at the ossification level. The developmental variations observed could be explained by considering the different embryonic origin and mode of ossification of the affected skeletal elements.
Objective: To evaluate the teratogenic effect of mobile phone radiation exposure during pregnancy on embryonic skeletal development at the common used mobile phone frequency in our environment. Methods: Sixty female Sprague-Dawley rats were distributed into three experiment groups; control and two exposed groups (1 h/day, 2 h/day exposure groups) (n=20/ each group) and exposed to whole body radiation during gestation period from day 1- day 20. Electromagnetic radiofrequency signal generator was used to generate 1 800 MHz GSM-like signals at specific absorption rate value 0.974 W/kg. Animals were exposed during experiment in an especial designed Plexiglas box (60 cm × 40 cm × 30 cm). At the end of exposure duration at day 20 of pregnancy animals were sacrificed and foetuses were removed, washed with normal saline and processed to Alizarin red and Alcian blue stain. Skeleton specimens were examined under a stereo microscope and skeleton's snaps were being carefully captured by built in camera fixed on the stereo microscope. Results: Intrauterine exposure to electromagnetic radiation lead to variation in degree of ossification, mineralization, formation of certain parts of the skeleton majorly in head and lesser in other parts. Deformity and absence of formation of certain bones in the head, ribs, and coccygeal vertebrae were recorded in skeleton of foetuses from exposed dams compare to control group. Conclusions: The electromagnetic radiation exposure during pregnancy alter the processes of bone mineralization and the intensity of bone turnover processes, and thus impact embryonic skeleton formation and development directly.
Renal Failure, 2014
Background: The aim of this study was to investigate the effects of exposure to a 900-MHz electromagnetic field (EMF) produced by mobile phones on the renal development of prenatal rats. Histopathological changes and apoptosis in the kidneys, together with levels of urea, creatinine and electrolyte in serum were determined. Methods: A total of 14 Sprague-Dawley rats were studied. Pregnant rats were divided into two equal groups: a control group and an EMF-exposed group. The study group was exposed to 900-MHz of EMF during the first 20 days of pregnancy, while the control group was unexposed to EMF. Sections obtained from paraffin blocks were stained for caspase-3 by immunohistochemistry, hematoxylin-eosin and Masson's trichrome. Results: Mild congestion and tubular defects, and dilatation of Bowman's capsule were observed in the kidney tissues of rats in the exposed group. Apoptosis was evaluated using anti-caspase-3; stronger positive staining was observed in the renal tubular cells in the study group than those of the control group. Although there was a significant difference between the study and control groups in terms of K + level (p50.05), no significant difference was observed in the other parameters studied (p40.05). Conclusion: Our study shows that the electromagnetic waves propagated from mobile phones have harmful effects on the renal development of prenatal rats.
The effects of radiofrequency radiation on mice fetus weight, length and tissues
Data In Brief, 2018
The publicconcernofharmfuleffectsofradiofrequencyradiation exposure,especiallywithrapidincreaseintheuseofwirelessand telecommunication devices,isincreasing.Somestudiesshowfetal and developmentalabnormalitiesastheresultofradiofrequency radiation exposure.Weaimedtoinvestigatepossibleteratogenic effects ofradiofrequencyin915MHzonmicefetusandprotective role ofvitaminC.21pregnantmiceweredividedinto3groups. Control groupwasinnormalconditionwithoutanystressoragent. Exposure groupwasexposedto915MHzRFR(8h/dayfor10days) and 0.045 mw/cm2 powerdensity.Theexposureplusvitamin C groupreceived200mg/kgvitaminCbygavageandwasexposed to 915MHzRFR(8h/dayfor10days)and0.045 mw/cm2 power density.Thefetusweight,C-Rlengthweremeasuredbydigital balance andcaliper.TissueswereassessedafterstainingwithH& E. Ourresultsshowedsignificant increaseinfetusweightandC-R length andalsoenlargedliver,taildeformationinmicefetusin exposuregroup.AlthoughusageofvitaminCcausedsignificant decrease inmentionedparameters.Theoutcomeofthisstudy confirms theeffectsofradiofrequencyradiationongrowthpara- meters suchasbodyweight,lengthandsometissuesinmice fetuses andprotectiveeffectofvitaminC.Howevermorestudies on non-ionizationradiationindifferentfrequenciesandseverity, during pregnancyareneededtoclarifytheexactmechanismsof these changesandbetterprotection.
Medical Express, 2015
BACKGROUND: The aim of this study was to determine whether shortwave electromagnetic radiation in pulsed mode with a frequency of 45 Hz, promotes teratogenic changes, stillbirths and changes in organ weight at birth in the fetuses of pregnant rats exposed to this radiation. METHOD: Ten black Macole pregnant female rats were studied, 5 in a test group, subjected to electromagnetic radiation with an average power of 4.5 W for 15 minutes, on a daily basis, during the entire period of pregnancy and 5 in a control group that was not exposed to radiation. At day 21, the animals were euthanized for analysis of fetuses. To assess the thermal effect of the radiation the abdominal temperature was measured before and after application, together with prevailing ambient temperature. The fetuses were evaluated through the Galant reflex to assess the existence of stillbirths. The fetuses were removed and weighed; through a midline laparotomy their hearts, stomachs, kidneys and livers were excised and weighed, wet and dry. Twenty-nine fetuses were harvested in the control group and 59 in the test group, with no cases of stillbirth, teratogenesis or malformation of internal organs. RESULTS: The analysis found no differences in birthweight and weight of dehydrated organs when compared to the control group. CONCLUSION: The results in our findings suggest that exposure to shortwave therapy without significant heat buildup, does not lead to teratogenic changes and did not affect the mass and weight of dehydrated internal organs.
Histological and Ultrastructural Analyses of Male Mice Exposed to Mobile Phone Radiation
ABSTRACT This study evaluated the possible toxicological effects of exposure to 900 MHz Global System for Mobile Communications (GSM) like radio frequency radiation (RFR) on the histology and ultrastructure of various body tissues of Balb/c mice. Thirty-six Balb/c male mice were randomly divided into two groups RFR-exposed and shamexposed. In triplicates of six, mice were exposed to FRF for 30 min/d for a month at whole body specific absorption rate (SAR) of 1 Watt/kg. The same process was applied to the sham group but the RFR generator was turned off. On day 30, brain, liver, ear skin, spleen, testes, kidney and retina were microscopically studied. RFR at the studied frequency did not cause significant histological changes in the tissues examined. The only exception to this conclusion is the noticeable changes in retinal histopathology. The findings of this and other similar studies, in many cases are controversial. But if some results are extrapolated to man, they must alert the scientific community to the possibility that RFR may have long-term deleterious effects.
Effect of cell phone radiation on reproductive system and behavior using female albino mice
2020
Aim of the work is to determine the behavioral effect and the histopathological changes of female reproductive system due to exposure to cell phone radiation using albino mice. Design of the work: Twelve female albino mice were divided into two groups (n=6). One group as control (healthy mice), the second group exposed to cell phone radiation one hour/day for three months. At the end of experiment, behavior scoring using plus maze and force swimming maze was applied for the two groups. Mice were killed by cervical dislocation, and dissected. The ovary and uterus collected and kept in formalin for histological examination. Results and Conclusion: Behavior study using plus maze and forced swimming maze did not show any changes after exposure to cell phone radiation for three months. In histological examination, the control ovary revealed that the ovary was covered by a single layer of simple cuboidal (germinal) epithelium; the ovarian parenchyma formed of cortex and medulla. In the ov...