Cystine, an essential determinant of protein tertiary structure, is also a target for electrochemical manipulation: Cyclotides (original) (raw)

Development of UHPLC and HPLC-electrochemical (EC) detection methods for the measurement of different cystine-containing peptides including simple peptides (l t thi) di i d tid (i li) li tid (t i d Methods HPLC System: Thermo S including: HPG 3600RS (glutathione), medium sized peptides (insulin), cyclic peptides (oxytocin and vasopressin) and peptides with cystine knots (cyclotides). Methods: UHPLC-and HPLC-based methods using electrochemical detection with a robust boron-doped diamond (BDD) working electrode are described. Results: The novel BDD working electrode extends the range of analytes capable of • HPG-3600RS pump • WPS-3000TRS autosa • 3000 TCC-3000RS co • DAD-3000RS diode ar • ECD-3000RS electroch boron doped diamond Results: The novel BDD working electrode extends the range of analytes capable of being measured by EC detection, making measurement of low levels of peptides possible. Filter Constant: Cell Clean: Cell Clean Potential: Cell Clean Duration: Figure 1. Structures of peptides used during method development.