New Localities of Ropalospora phaeoplaca in Japan and Far East of Russia with ITS nrDNA Sequences (original) (raw)
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Mycological Progress, 2019
Roussoella species mainly occur on monocotyledons, especially bamboo, and are characterized by raised, immersed ascostromata, cylindrical to clavate asci and ellipsoidal to fusiform, brown to dark brown, 1-septate, ornamented ascospores. They have cytospora-like asexual morphs with enteroblastic conidiogenous cells and hyaline to dark brown conidia, which are often minutely warty. In this article, two new species and two new records of Roussoella associated with dead bamboo, collected from Yunnan Province of China, are described and illustrated. These four taxa are similar in morphological characteristics, but can be distinguished by phylogenetic analyses of the concatenated ITS, LSU, TEF1-α, and RPB2 sequence data. Based on maximum likelihood and Bayesian inference analyses of the combined sequence dataset, we introduce Roussoella kunmingensis and R. yunnanensis spp. nov. Roussoella mukdahanensis is provided here as the first record on bamboo from China. The asexual morph of R. pseudohysterioides is illustrated and described on dead bamboo culms.
Journal of Phycology, 2001
To better assess the current state of phaeophycean phylogeny, we compiled all currently available rbc L, 18S, and 26S rDNA sequences from the EMBL/GenBank database and added 21 new rbc L sequences of our own. We then developed three new alignments designed to maximize taxon sampling while minimizing information loss due to partial sequences. Phylogenetic analyses were performed on separate and combined data sets (with and without taxa from the sister classes Tribophyceae and Phaeothamniophyceae as outgroups) using a variety of assumption sets, tree-drawing algorithms (parsimony, neighbor joining, and likelihood), and resampling methods (bootstrap, decay, jackknife). Partition homogeneity testing (PHT) by codon position within rbc L showed that all positions could be used despite mild third position saturation. PHT by gene and domain within rDNA showed that the 26S D1 and D2 regions do not enhance phylogenetic signal even when combined with the 18S. The rbc L and rDNA (excluding the 26S D1 and D2) could be combined under PHT. The topology of the combined tree was the same as that of the rbc L tree alone, but bootstrap support was consistently higher in the combined analysis, applied to more branches, and enabled the establishment of sister group relationships among six orders. Although the taxon sampling for the combination tree was lower ( n ϭ 22) than for individual gene analyses ( n ϭ 58 for rbc L and n ϭ 59 for rDNA), results show that the Laminariales (previously reported) and Sphacelariales (new) are both paraphyletic. Choristocarpus tenellus (Kützing) Zanardini is the most basal phaeophyte and the Dictyotales the most basal order. In contrast, the Laminariales sensu stricto ( s.s. ) and Ectocarpales sensu lato ( s.l. ) are the most derived. For phylogenetic studies in the Phaeophyceae, rbc L has more resolving power than rDNA, though the reason for this is unclear based on the fact that both genes are highly conserved.
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Zeitschrift für Naturforschung. C, Journal of biosciences
Like many lichen-forming fungi, species of the genus Rhizoplaca have wide geographical distributions, but studies of their genetic variability are limited. The information about the ITS rDNA sequences of three species of Rhizoplaca from Anatolia was generated and aligned with other species from other countries and also with the data belonging to Lecanora species. The examined species were collected from the volcanic rocks of Mount Erciyes which is located in the middle of Anatolia (Turkey). The sequence data aligned with eight other samples of Rhizoplaca and six different species of Lecanora were obtained from GenBank. The results support the concept maintained by Arup and Grube (2000) that Rhizoplaca may not be a genus separate from Lecanora. According to the phylogenetic tree, Rhizoplaca melanopthalma from Turkey with two different samples of R. melanopthalma from Arizona (AF159929, AF159934) and a sample from Austria formed a group under the same branch. R. peltata and R. chrysol...
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Fems Microbiology Letters, 1995
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Mycoscience, 2007
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Phylogenetics of Ruscaceae sensu lato based on plastid rbcL and trnL-F DNA sequences
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