A Comparative Study of the Lipophilicity of Metformin and Phenformin (original) (raw)
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International Journal of Trend in Scientific Research and Development, 2019
A simple and reproducible method was developed for Metformin (MET) by Reverse Phase High Performance Liquid Chromatography (RP-HPLC). Metformin was separated on C18 column [4.6x250mm, particle size 5μm], using combination of phosphate buffer with pH of 3.0 and Methanol at the UV detection of 238nm. Isocratic elution of phosphate buffer with pH of 3.0 and Methanol was used as a mobile phase with various ratios and flow rates, eventually 30:70 v/v phosphate buffer with pH of 3.0 and Methanol was being set with the flow rate of 1mL/min. The statistical validation parameters such as linearity, accuracy, precision, inter-day and intra-day variation were checked, assay studies of Metformin were within 98% to 102% indicating that the proposed method can be adoptable for quality control analysis of Metformin.
Journal of Scientific Research, 2013
A rapid and sensitive isocratic reversed phase high performance liquid chromatographic method has been developed for quantitative analysis of metformin hydrochloride and gliclazide in bulk as well as pharmaceutical dosage forms. The method was validated according to FDA, ICH and USP guidelines with respect to accuracy, precision, specificity and linearity. For metformin, the method was developed by using the mobile phase comprising of sodium dihydrogen phosphate solution (NaH 2 PO 4) (pH 3.0) and acetonitrile (90:10, v/v) at a flow rate of 0.5 mL/min over C 18 bonded silica column (3.9 x 300 mm, 5 µm) at ambient temperature. On the other hand, the same mobile phase was used in the ratio of 20:80 (v/v) at a flow rate of 0.6 mL/min for gliclazide. The recovery was found to be more than 97% for metformin and 102% for gliclazide that demonstrated the accuracy of the protocol. Intraday and interday precisions of the new method were less than the maximum allowable limit (%RSD 2.0) specified by the USP, ICH and FDA. The method revealed linear response with correlation coefficient value of 0.999 in both cases.
Current Pharmaceutical Analysis, 2012
A simple stability-indicating high-performance liquid chromatographic method was developed and validated for the determination of Cabazitaxel in infusion forms. Reversedphase chromatography was performed on Shimadzu Model CBM-20A/20 Alite, using a mixture of Phosphate buffer and Acetonitrile (30:70, v/v) as mobile phase with a flow rate of 1.0 mL/min. Detection was carried at 230 nm. Linearity was observed over the concentration range of 0.1-150 μg/mL (R 2 = 0.9999) with regression equation y = 28290 x + 7032.3. Cabazitaxel was subjected to stress conditions (acidic, alkaline, oxidation, UV and thermal degradation) and validated as per ICH guidelines.
Chemical Industry and Chemical Engineering Quarterly, 2013
The retention of some 5,5-disubstituted hydantoins was investigated by reversed phase high performance thin-layer chromatography (RP HPTLC) and reversed phase high-pressure liquid chromatography (RP HPLC). The mobile phases were mixtures of methanol-water and acetonitrile-water in various volume fractions. In order to explore and visualize similarities and differences among the investigated compounds and chromatographic system Principal Component Analysis (PCA) was used. Results show that the experimental lipophilicity indices estimated from retention data (RM,W, logkw) and PC1 are directly correlated with logP values at a high significant statistical level.
International Journal of PharmTech Research, 2020
A Simple, rapid, cost effective, stability indicating RP-HPLC method has been developed for separation of Metformin HCl, its related impurities and Acarbose. Validated the method for simultaneous estimation of Metformin (MF) and Acarbose (ACB) in its novel combination of tablet formulation with Metformin 500 mg and Acarbose 50 mg. Metformin HCl is an orally-administered biguanide, anti-hyperglycemic agent, used in the management of non-insulin dependent diabetes mellitus. Acarbose is an oligosaccharide, used orally for the treatment of type 2 diabetes mellitus. The separation was achieved by using isocratic mobile phase consisting of mixture of phosphate buffer : acetonitrile (27:73 v/v), using Hypersil APS2 column, (250 x 4.6 mm x 5m) column at flow rate 2.0 mL/min. The detection was carried out at 210 nm with 20 μl of injection volume. The column temperature was maintained at 35 °C. The retention time (RT) of MF, its related impurities and ACB were found to be RT 2.6 min for 1-Cy...
Indian Journal of Pharmaceutical Education and Research, 2020
Background: A novel stability indicating analytical method was developed and validated by High Performance Thin Layer Chromatography (HPTLC) using Design of experiment approach. The proposed method is useful for quantification of Metformin hydrochloride and Empagliflozin in bulk and its dosage forms simultaneously. Design of experiment approach was applied for optimization of chromatographic conditions. Materials and Methods: For optimization process independent variables were used as Isopropyl alcohol proportion in mobile phase, saturation time of chamber and distance travelled by mobile phase. Experiments were carried out on silica gel pre-coated plate using mobile phase as 2 % Ammonium acetate: Isopropyl alcohol: Triethylamine (4:6:0.1 v/v/v). Direct evaluation of chromatograms were done by TLC scanner with reflectance/absorbance mode set at 242 nm. Method was validated as per ICH Q2 (R1) requirements. Results: Correlation coefficients for calibration curves were found to be 0.98...
Asian Journal of Pharmaceutical and Clinical Research, 2016
Objective: To develop accurate, fast, simple, and precise reversed-phase high-pressure liquid chromatography method for simultaneous determination of the binary mixture of metformin (MET) and empagliflozin (EMPA) in dosage forms. Methods: The method uses a mobile phase consisting of phosphate buffer, acetonitrile, methanol (15:80:5 v/v/v), an octadecyl silica C-18 column (4.6 mm × 250 mm, 5 μ particle size) in isocratic mode, detection wavelength of 227 nm, and a flow rate of 1 mL/minutes. Results: The measured retention times for MET and EMPA and were 2.528 and 4.140 minutes, respectively. The percentage recoveries of MET and EMPA were 101.12% and 100.55%, respectively. The relative standard deviation for assay of tablets was found to be <2%. The correlation coefficient for MET and EMPA was found to be 0.9990. The limit of detection and quantification for MET was 0.10 µg/mL and 0.31 µg/mL and for EMPA 0.01 µg/mL and 0.03 µg/mL. Conclusion: The method was f...
Analytica Chimica Acta, 2006
The chromatographic conditions aiming to a better simulation of n-octanol-water partitioning using a base deactivated silica (BDS) column as stationary phase were investigated for structurally diverse basic and neutral drugs. Extrapolated retention factors log k w , determined using different methanol fractions as organic modifier, were considered as lipophilicity indices. The effect of n-decylamine and n-octanol as mobile phase additives was examined and the appropriateness of the final retention outcome to reproduce lipophilicity data was evaluated. Moreover, the influence of n-octanol on the linearity of the log k/methanol fraction relationship and on the uniformity of the retention mechanism was investigated. 1:1 correlation between log k w values and the logarithm of the distribution coefficient (log D) was established for basic drugs in presence of both n-decylamine and n-octanol as mobile phase additives. However, for neutral drugs n-decylamine proved to be a sufficient and more important factor than n-octanol.
GSC Biological and Pharmaceutical Sciences, 2020
In Mexico, Metformin (MTF) is an oral hypoglycemic drug that is part of the group of biguanides used as a first-line treatment for the management of Type 2 Diabetes Mellitus. In the Mexican pharmaceutical market, there is a wide variety of medicines containing this drug, hence the importance of evaluating the quality of the medicines sold in the country. One of the important chemical tests performed both in the drug development phase, and in the quality evaluation criteria during its production, is the dissolution test. This is the test where it simulates in vitro, the time it takes for a given drug to pass into its soluble form, thus establishing its release time. The dissolution test to assess bioavailability in vitro has already been included in several official regulations, as a quality control method that allows predicting the behavior of the drug in the pharmaceutical form after its administration. The aim of this work was to validate a simple and reliable analytical method to quantify MTF in immediate-release tablets by UV spectroscopy, for a comparative study of in vitro dissolution profiles. In dissolution medium of 0.68% potassium monobasic phosphate at pH 6.8, adjusted with sodium hydroxide, the method demonstrated linearity in the range of 2 to 16 µg/L. The parameters evaluated complied with the provisions of the national and international guidelines for the validation of analytical methods; therefore, the analytical method meets the requirements for the quantification of Metformin in dissolution studies.