Colonization by Porphyromonas gingivalis and Prevotella intermedia from teeth to osseointegrated implant regions (original) (raw)
Related papers
Brazilian Journal of Microbiology, 2005
The colonization and antimicrobial susceptibility of P. intermedia and P. gingivalis isolated from peri-implant and gingival sulcus samples were determined. Samples were collected from 30 patients submitted to implant in three different times: at the moment of the surgery, 20 and 60 days after the implant installation. Organisms were identified by using a biochemical tests or API 32-A kit and by PCR. The antimicrobial susceptibility was determined by using an agar dilution method. Nineteen P. intermedia (4 from peri-implant sites and 15 from gingival sulcus), and only seven P. gingivalis from gingival sulcus were isolated. Organisms were detected by PCR from seven peri-implant and 32 gingival samples. Bacteria were susceptible to the used antibiotics except to azithromycin with 65% of resistance for P. intermedia strains. Both tested species were susceptible to cadmium, nickel and palladium, and showed different resistance rates to titanium, aluminum and mercuric chloride. Most of P. intermedia strains were resistant to lead, silver, copper, titanium, zinc, aluminum and mercuric chloride. Bacteria colonized implants after 60 days of surgery and PCR may be used as a tool for bacterial detection in implantology.
Transmission of Periodontopathic Bacteria from Natural Teeth to Implants
Clinical Implant Dentistry and Related Research, 2012
Purpose: Prevention of peri-implantitis is essential for the success of implant rehabilitation. Infection by periodontopathic bacteria is a major cause of peri-implantitis. The aim of the present study was to identify the source of periimplant colonization by periodontopathic bacteria. Materials and Methods: Twenty-one patients with implants were enrolled in the study. Subgingival plaque samples from the adjacent, occluding, and contralateral natural teeth were collected prior to second-stage surgery. Samples from implant sulci were then obtained 2 weeks later. Detection of periodontopathic bacteria was performed by the polymerase chain reaction. Results: The detection rates for Aggregatibacter actinomycetemcomitans, Prevotella intermedia, Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia and Fusobacterium nucleatum in all subgingival samples from natural teeth were similar to that in the peri-implant sulci. Multiple logistic regression analysis revealed an association between the detection of A. actinomycetemcomitans, P. intermedia, P. gingivalis, T. denticola and F. nucleatum in the gingival crevices of adjacent teeth and that of the peri-implant sulcus, but no association for T. forsythia. Conclusions: The present findings suggest that colonization by A. 3 actinomycetemcomitans, P. intermedia, P. gingivalis, T. denticola and F. nucleatum at the implant sulcus was affected by these microorganisms in the gingival crevice of adjacent teeth rather than those on occluding and contralateral teeth.
2018
Dental implants provide excellent results in terms of survival and the success rates of oral rehabilitation. The Dental Teaching Hospital, Faculty of Dentistry, Universitas Indonesia (DTH UI) is among the leading dental hospitals that have offered dental implants since 2009, but dental implant treatments have not yet been fully evaluated. The aim of this study was to evaluate the implant success rate by quantification of levels of Porphyromonas gingivalis bacteria. Twenty-nine dental implant samples were taken from patients from the Periodontal Clinic of DTH UI from 2009–2014. Samples plaques were obtained from each dental implant using implant probes. The baseline group consisted of similar plaque samples taken from healthy teeth and periodontitis teeth. All samples were subjected to microbiological analysis by quantification of P. gingivalis using real time PCR. No significant differences were noted in numbers of P. gingivalis between the dental implant groups and the healthy toot...
Assessment of clinical and microbiological status of dental implant And adjacent teeth
2018
Background: Dental plaque carries numerous bacteria which are harmful for the normal health of gingiva. Dental implants are widely used in dentistry. There are various factors which affects the outcome of implant therapy. The present study was conducted to assess the presence of bacteria around dental implants. Materials & Methods: The present study was conducted on 20 patients who received dental implants in the last 2 years. They were divided into 2 groups of 10 patients each. Group I comprised of patients in which subgingival plaque sample was obtained around dental implant and group II had those patients in which subgingival plaque sample was obtained around teeth adjacent to dental implant. Samples were subjected to microbiological analysis using PCR. In all patients, plaque index, sulcus bleeding index and probing pocket depth was measured. Results: In group I, mean plaque score for P. gingivalis was 2.16 and in group II was 1.78. The difference was nonsignificant (P> 0.05)...
Clinical Oral Investigations, 2016
Objectives The aim of the present study was to compare the composition of the periodontal microflora at baseline (T0) with the submucosal microflora at least 1 year after implant placement (T1) in periodontally healthy patients. Material and methods For all 169 consecutive patients that visited our clinic during 1 year, we determined their periodontal parameters, implant mucosal index, and presence of implant calculus. At T0, self-reported smoking status was recorded and subgingival and submucosal biofilm samples were obtained and analyzed for the presence and numbers of selected periodontal pathogens. All measurements were repeated at T1. Results One hundred twenty patients completed the study. Periodontal parameters were stable or had improved at T1. The total bacterial load was lower at implant sites (P < 0.05). The prevalence of Porphyromonas gingivalis was low at baseline, but at T1, detection rate and numbers were higher at implant sites compared to dentate sites. At T1, the frequency of detection of P. gingivalis (P = 0.01), Parvimonas micra (P = 0.018), and Fusobacterium nucleatum (P = 0.035) was higher in smoking patients (n = 23) than in non-smokers (n = 97). Conclusions Colonization of the submucosal peri-implant area is similar to the composition of subgingival microbiota. Smoking has a measurable effect on the colonization of implant-associated biofilms and may select for P. gingivalis, P. micra, and F. nucleatum. Clinical relevance The colonization of implants by wellknown periodontal pathogens is very similar to that in normal dentition, also in a healthy cohort. Smoking status was related with the prevalence of periodontal pathogens where smokers harbored more often periodontal pathogens such as P. gingivalis, P. micra, and F. nucleatum.
Applied Sciences
Even though most studies consider strict anaerobe Gram-negative bacteria as the main factor associated with peri-implantitis, other studies have identified other microorganisms present in implants related to peri-implant disease that have the ability to reduce the effectiveness of treatment, such as Candida spp., Enterococcus faecalis and Pseudomonas aeruginosa. Therefore, microbiologic diagnosis is important for the success of implant treatment. The main goal of this study was to detect Candida spp., E. faecalis and P. aeruginosa in the peri-implant and periodontal subgingival plaque in the presence or absence of disease and to relate the presence of these microorganisms with demographic data, hygiene habits, the type of implant connection and endodontic treatment. The study population consisted of 20 patients that filled out a questionnaire regarding gender, age, systemic diseases and oral hygiene. The peri-implant and periodontal subgingival plaque from an adjacent tooth, both wi...
Biomarkers and Bacteria Around Implants and Natural Teeth in the Same Individuals
Journal of Periodontology, 2017
Aim: This cross-sectional study assessed cytokine levels in peri-implant crevicular fluid (PICF)/ gingival crevicular fluid (GCF) and a selection of subgingival/submucosal plaque bacteria from clinically healthy or diseased sites in the same individuals. Material and Methods: Samples from 97 implants/teeth (58 implants: 19 healthy, 20 mucositis, 19 periimplantitis; 39 natural teeth: 19 healthy, 12 gingivitis, 8 periodontitis) in 15 systemically healthy patients were investigated by immunoassay, real-time PCR. Samples were obtained first and then probing depth, clinical attachment level, bleeding on probing, plaque index scores, keratinized tissue width were recorded. Data were analyzed by Wilcoxon, Mann-Whitney and permutation tests on dependent, independent, mixed dependent and independent samples and Spearman correlation. Results: Interleukin-1beta levels were significantly higher in PICF samples of healthy implants than in GCF samples of healthy teeth (p=0.003), soluble activator of nuclear factor Kappa-B (sRANKL) concentrations were significantly higher in gingivitis than mucositis group (p=0.004). The biomarker levels were similar in periimplantitis and periodontitis groups (p>0.05). Actinomyces naeslundi and Streptococcus oralis levels were significantly higher in healthy implant group than healthy teeth (p<0.05). Prevotella intermedia and Treponema denticola levels were lower in mucositis group than in gingivitis group (p<0.05). Prevotella oralis and S. oralis levels were significantly higher in the periodontitis group (p<0.05) and T. denticola levels were significantly higher in the peri-implantitis group (p<0.05). There were many similarities but crucially some differences in biomarker levels (IL-1β and sRANKL) and bacterial species between peri-implant and periodontal sites in the same individuals suggesting similar pathogenic mechanisms.
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 2007
Black-pigmented anaerobic rods such as Prevotella spp. and Porphyromonas spp. are involved in the etiology and perpetuation of endodontic infections. The aim of this study was to evaluate the prevalence of these species in chronic endodontic infections by using culture and polymerase chain reaction (PCR) techniques. Samples of 100 patients with root canals displaying chronic endodontic infections were obtained by sterilized paper points. Bacterial identification was performed by using culture and PCR techniques. By culture, in 33% of the samples, P. intermedia-P. nigrescens (75.8%), P. gingivalis (27.3%), and P. endodontalis (9.1%) were identified, and by PCR 60% of the samples harbored P. nigrescens (43.3%), P. gingivalis (43.3%), P. intermedia (31.7%), and P. endodontalis (23.3%). The presence of these black-pigmented anaerobic rods alone or in association in chronic endodontic infections seems to be frequent. PCR is a very sensitive technique for detecting DNA from bacterial cells. Culturing is only able to reveal living bacteria and is less sensitive for the identification of low numbers of bacterial cells.