Biocompatible nanostructured solid adhesives for biological soft tissues (original) (raw)
Related papers
Biocomposites of nanohydroxyapatite with collagen and poly(vinyl alcohol)
Colloids and Surfaces B: Biointerfaces, 2006
Biocomposites of hydroxyapatite, HAp, in conjunction with various binders including poly(vinyl alcohol), PVA, and collagen have the potential of serving in various tissue engineering applications, such as in bone repair and reconstruction tasks, especially if the nanoparticles of hydroxyapatite are used. Here, hydroxyapatite nanoparticles (n-HAp) were synthesized at the ultimate size range of 10-50 nm and then incorporated into PVA or in situ synthesized in collagen/PVA. The biocomposites of HAp with PVA exhibited relatively high elasticity (as revealed by the linear viscoelastic material functions, characterized upon small-amplitude oscillatory shear) especially upon cryogenic treatment. The incorporation of the collagen into the PVA/HAp biocomposite provided internal porosity to the biocomposite with the pores in the 50-100 nm range for collagen/HAp and 50-500 nm for the collagen/HAp/PVA.
Development of a Hydroxyapatite/Collagen Nanocomposite as a Medical Device
Cell Transplantation, 2004
The effect of cross-linking of a hydroxyapatite/collagen (HA/Col) nanocomposite, in which HA nanocrystals and collagen fibers are aligned like natural bone by a self-organization mechanism between HA and collagen in vitro, on mechanical properties was examined. The influence of degree of cross-linking, as well as rhBMP-2 preadsorption to the composite on the substitution pattern and rate with bone, was examined. In Experiment 1, anterior fusion was carried out at the C3–C4 vertebrae on 10 dogs and they were implanted as follows: without cross-linking and without adsorbed rhBMP-2 (three dogs), with cross-linking and without adsorbed rhBMP-2 (three dogs), without cross-linking and with adsorbed rhBMP-2 (two dogs), and with cross-linking and adsorbed rhBMP-2 (two dogs). Implants were removed from each dog for histology determinations after 12, 16, and 24 weeks in the non-rhBMP-treated groups, and after 16 and 24 weeks in the rhBMP-treated groups. In Experiment 2, the HA/Col composites ...
Materials, 2021
Regenerative medicine is becoming a rapidly evolving technique in today’s biomedical progress scenario. Scientists around the world suggest the use of naturally synthesized biomaterials to repair and heal damaged cells. Hydroxyapatite (HAp) has the potential to replace drugs in biomedical engineering and regenerative drugs. HAp is easily biodegradable, biocompatible, and correlated with macromolecules, which facilitates their incorporation into inorganic materials. This review article provides extensive knowledge on HAp and collagen-containing compositions modified with drugs, bioactive components, metals, and selected nanoparticles. Such compositions consisting of HAp and collagen modified with various additives are used in a variety of biomedical applications such as bone tissue engineering, vascular transplantation, cartilage, and other implantable biomedical devices.
Hydroxyapatite Nanoparticle Coating on Polymer for Constructing Effective Biointeractive Interfaces
Journal of Nanomaterials, 2019
Bone is an organic-inorganic composite with the ability to regenerate itself. Thus, several studies based on artificial organic-inorganic interface sciences have been tried to develop capable materials for effective regenerative bone tissues. Hydroxyapatite nanoparticles (HAp NPs) have extensively been researched in bone tissue engineering due to the compositional and shape similarity to the mineral bone and excellent biocompatibility. However, HAp alone has low mechanical strength, which limits its applications. Therefore, HAp NPs have been deposited on the biocompatible polymer matrix, obtaining composites with the enhanced mechanical, thermal, and rheological properties and with higher biocompatibility and bioactivity. For developing new biomedical applications, polymer-HAp interfacial interactions that provide biofusion should be investigated. This paper reviewed common coating techniques for obtaining HAp NPs/polymer fusion interfaces as well as in vitro studies of interfacial ...
Poly(vinyl alcohol)/collagen/hydroxyapatite hydrogel: Properties and in vitro cellular response
Journal of Biomedical Materials Research Part A, 2012
The objective of this study was to develop ''bonelike'' poly(vinyl alcohol) (PVA)/hydroxyapatite (HA)/type I collagen (Col) hydrogel composites that stimulate adhesion, proliferation, and differentiation of osteoblastic cells. The hydrogel composites were prepared by mixing PVA with nanoscale HA and Col using a physical mixing method. The concentration of the components was optimized during formulation development. PVA/Col/HA hydrogels were characterized for viscoelasticity, degree of swelling, mechanical strength, embedded erythromycin drug release, and cellular response of both osteoblastic MC3T3 cells and RAW 264.7 macrophage cells. Compressive strength tests confirmed that the PVA coating possessed greater elasticity and was mechanically enhanced by the freeze-thaw treatment. PVA/ Col/HA gel is biocompatible and nontoxic to MC3T3 preosteoblasts, and the reinforcement from HA and Col reduced the inflammatory response from macrophages. Our findings demonstrate that PVA composites are biocompatible, and enhance cell adhesion, proliferation, and differentiation in vitro. We propose that PVA/Col/HA hydrogels represent one of the promising implant surface coating matrices for the improvement of implant osseointegration. V C 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 00A: 000-000, 2012.
Biocompatible hybrid allyl 2-cyanoacrylate and hydroxyapatite mixed for bio-glue
Journal of Adhesion Science and Technology, 2016
Despite cyanoacrylate's numerous advantages such as good cosmetic results and fast application for first aid, drawbacks such as brittleness and local tissue toxicity have limited their application. In this study, to improve both the biocompatibility and mechanical properties of cyanoacrylate, allyl 2-cyanocarylate was pre-polymerized and mixed with poly(L-lactide-co-ε-caprolactone) (PLCL, 50:50) as biodegradable elastomer and mixed with hydroxyapatite (HA) as multifunctional bio-glue for variety body application. Hybridized allyl 2-cyanoacrylate (Hybridized Cy) was mixed with pre-polymerization allyl 2-cynaocarylate and PLCL. For the evaluation of various properties of hybridized Cy/HA bio-glue mixtures, bond strength, surface analysis (SEM, XRD), cell viability, and biocompatibility were confirmed. Especially, optimal condition for hybridized Cy/HA bio-glue was determined as 1.2 wt% HA mixture ratio through bond strength. Surface morphology has smooth form to increase HA mixture ratio. Cell viability increased with optimal ration of hybridized Cy/HA. These results indicate that hybridized Cy/HA bio-glue can be used widely as advanced adhesive in various fields.
Acta Biomaterialia, 2008
Many studies are currently underway on the quest to make synthetic bone-like materials with composites of polymeric materials and hydroxyapatite (HA). In the present work, we use wetting experiments and surface tension measurements to determine the work of adhesion between biodegradable polymers and HA, with specific reference to the role of humid environments. All the polymers are found to exhibit low contact angles (660°) on the ceramic with work of adhesion values ranging between 48 J m À2 for poly(e-caprolactone) and 63 J m À2 for polylactide; these values are associated with physical bonding across the organic/inorganic interface. The corresponding mechanical fracture strengths, measured using four-point bending tests of HA-polymer-HA bonds, scale directly with the results from the wetting experiments. Short-time aging (up to 30 h) in a humid environment, however, has a dramatic influence on such HA/polymer interfacial strengths; specifically, water diffusion through the organic/inorganic interface and degradation of the polymer results in a marked decrease, by some 80-90%, in the bond strengths. These results cast doubt on the use of biodegradable polymers/ceramic composites for load-bearing synthetic bone-like materials, as desired optimal mechanical properties are unlikely to be met in realistic physiological environments.
Colloids and surfaces. B, Biointerfaces, 2018
Nano sized bio-composites containing inorganic particles conjugated with polymer and protein are considered as potential material for tissue engineering systems like bone repair and advanced drug delivery. More specifically, hydroxyapatite (HAp), a well known as the strong bioactive material has limitations on reactivity towards biological systems. Thus, this work explains the interaction betweena natural biomaterial Collagen and poly (lactide co-glycolide)-Hydroxyapatite (HAp) composite. PLGA/HAp composite was fabricated by in-situ polymerization of DL-lactide, glycolide and HAp nanoparticles. The prepared PLGA/HAp composite was examined for physico-chemical properties by FTIR, DSC, SEM, and DLS. The microscopic image confirms the positioning of a highly ordered structure containing Coll-PLGA/HAp that leads to enhancement in thermal stability of collagen. The nature of bonding and structural orientation of bio-composite was thoroughly investigated by FTIR and SEM. Toxicity of bio-c...
Biotechnology and Applied Biochemistry, 2015
In this study, three-dimensional hydroxyapatite/silk fibroin (HAp/SF) nanocomposite scaffolds were successfully prepared through layer solvent casting combined with freeze drying technique for tissue engineering applications. Various SF aqueous concentrations, ranging from 2.5 to 10%, were used to control the physico-chemical properties of the prepared scaffolds. Biologic responses of the rat bone marrow stromal cells (BMSCs) to the HAp/SF scaffolds were examined by culturing the cells within them. In addition, biodegradation and biocompatibility of the scaffolds were evaluated in vitro and in vivo, respectively. Among the prepared scaffolds, HAp/SF-2.5% was the most brittle sample, and showed porous structure with lowest mechanical properties. The average pore diameters decreased with the increase of the SF concentration from 5% to 10%, and were 350 ± 67 µm and 112 ± 89 µm, respectively. The pores formed in the scaffolds made up of the 5% SF were more uniform and regular than those of the scaffolds made of 5 and 10%. The HAp/SF scaffolds did not change the BMSCs viability and was not cytotoxic compared to the control sample. The SEM micrographs showed that the cells migrated into the pores and wellattached to the scaffolds and their cytoplasm was extended in all directions, indicating a promising cell adhesion, high biocompatibility and no cytotoxicity of the HAp/SF-5% nanocomposite scaffolds. Subcutaneous implantation of the HAp/SF-5% scaffolds in rat models suggested an excellent biocompatibility. All data obtained from this study suggest the potential use of the HAp/SF-5% for hard tissue engineering.