Antibacterial and antioxidant activities of extracts from Calophyllum ferrugineum and Calophyllum incrassatum (original) (raw)
Related papers
2019
Calophyllum is a pan-tropical genus belongs to the Guttiferae family and locally known in Malaysia as ‘bintangor’. There has been a continual interest to further investigate the phytochemistry of Calophyllum sp because this genus is a rich source of active secondary metabolites which shows anti-HIV, cytotoxicity and antimicrobial properties. In this study, antibacterial and antioxidant activities of barks and leaves of C. ferrugineum and C. incrassatum were investigated. Cold extraction method employing dichloromethane, ethyl acetate and methanol as solvent was performed. All extracts were tested for their total phenolic content and antioxidant activities by DPPH radical scavenging and Ferric Reducing Antioxidant Power (FRAP) assays. The methanol extract from the leaves of C. ferrugineum showed the highest TPC value at 122.08 mg GAE/g and the lowest DPPH SC50 value at 11.80 μg/mL. The methanol extract from the barks of C. ferrugineum was found to have the highest FRAP value among al...
2019
Calophyllum is a pan-tropical genus belonging to the Guttiferae family and is known in Malaysia as ‘bintangor’. There has been continual interest to further investigate the phytochemistry of Calophyllum species since this genus is a rich source of active secondary metabolites, which show anti-HIV, cytotoxicity, and antimicrobial properties. This study was conducted to investigate the effect of extraction techniques on the phytochemicals content and antioxidant activity of the barks, leaves, and heartwood extracts of three Calophyllum species, namely C. incrassatum, C. rubiginosum, and C. canum. Soxhlet and maceration extraction techniques by using methanol as solvent were chosen in this study. The maceration extraction technique produced higher percentage yield as compared to Soxhlet extraction for leaves and barks of the three Calophyllum species. The highest percentage yield was obtained from the bark extract of C. canum (21.76%), followed by the bark extract of C. rubiginosum (20...
Comparison of extraction techniques for three Calophyllum species and their antioxidant activity
2018
Calophyllum is a pan-tropical genus belongs to the Guttiferae family and locally known in Malaysia as ‘bintangor’. There has been a continual interest to further investigate the phytochemistry of Calophyllum sp since this genus is a rich source of active secondary metabolites which showed anti-HIV, cytotoxicity and antimicrobial properties. This study was conducted to investigate the effect of extraction techniques on the phytochemicals content and antioxidant activity of the barks, leaves and heartwood extracts of three Calophyllum species, C. incrassatum, C. rubiginosum and C. canum. Soxhlet and maceration extraction techniques by using methanol as solvent were chosen in this study. Maceration extraction technique produced higher percentage yield compared to Soxhlet extraction for leaves and barks of the three Calophyllum species. Highest percentage yield was obtained from bark extract of C. canum (21.76%) followed by bark extract of C. rubiginosum (20.24%) and leaves extract of C...
The phytochemical content and antimicrobial activities of Malaysian Calophyllum canum (stem bark)
Pakistan journal of pharmaceutical sciences, 2012
Recently there was huge increase in using of 'herbal products'. These can be defined as plants, parts of plants or extracts from plants that are used for curing disease. However, Calophyllum species is a tropical plant and it has been used in traditional medicine, the limitation in safety and effectiveness information could lead to serious health problems. Providing information for communities by evaluating the phytochemical contents, antioxidant, antimicrobial and cytotoxic activities will improve the therapeutic values. Three main Calophyllum canum fractions (none - high polar) were tested to find out the phenolic, flavonoid, flavonol content, DPPH radical scavenging, reducing power and chelating iron ions. Also were tested against Bacillus cereus, Staphylococcus aureus, Escherichia coli, Psedomonas aeruginosa, Candida albicans, and Cryptococcus neoformans. In addition, cytotoxic activity was assayed against lung cancer A549 cell line. The methanol fraction showed no bioac...
Anti-oxidant and anti-bacterial activities of 11 Calophyllum species from Indonesia
Journal of Microbial Systematics and Biotechnology, 2019
Members of Calophyllum (Calophyllaceae) are commonly used as herbal medicine in Indonesia from the beginning of civilization. Due to their tremendous potential as a source for pharmaceutical industry, we selected 11 Calophyllum species for the current anti-oxidant and anti-bacterial studies, namely,
Calophyllum brasiliense (Clusiaceae/Guttiferae) is a native Brazilian medicinal plant traditionally used against several diseases, including infectious pathologies. Crude methanolic extracts (CME) and two fractions, denoted non-polar (soluble in chloroform) and polar (nonsoluble in chloroform), were prepared from different parts of the plant (roots, stems, leaves, flowers and fruits) and studied. The following compounds were isolated and tested against pathogenic bacteria and yeasts by determination of the minimal inhibitory concentration (MIC): brasiliensic acid (1), gallic acid (2), epicatechin (3), protocatechuic acid (4), friedelin (5) and 1,5-dihydroxyxanthone (6). The results indicated that all the parts of the plant exhibited antimicrobial activity against Gram-positive bacteria, which are selectively inhibited by components of C. brasiliense. No activity was observed against Gram-negative bacteria and yeasts tested. Regarding the isolated compounds, substance 4 showed antimicrobial activity against all the tested microorganisms, whereas compound 6 exhibited antimicrobial activity only against Gram-positive bacteria. The results from the current study confirm and justify the popular use of this plant to treat infectious processes.
Cytotoxic and antibacterial activities of constituents from Calophyllum ferrugineum ridley
Records of Natural Products, 2016
This study evaluated the chemical composition of Calophyllum ferrugineum, cytotoxicity against human breast cancer (MCF-7) and human lung carcinoma (A-549) cell lines as well as antibacterial activities against two Gram-positive bacteria, S. aureus and B. subtilis and two Gram-negative bacteria, P. aeruginosa and E. coli. Phytochemical investigations of the bark extract yielded isoapetalic acid (1), apetalic acid (2), 6-hydroxy-2-methoxyxanthone (3) and ent-epicatechin (4). Meanwhile, betulinic acid (5), protocatechuic acid (6) and amentoflavone (7) were isolated from the leave extract. Isoapetalic acid (1) and apetalic acid (2) exhibited cytotoxic activities towards both cancer cell lines and both Gram-positive bacteria. Compounds (3-7) were inactive or showed moderate activities towards cytotoxic and antibacterial tests. This study presents the first report on the phytochemicals investigation from C. ferrugineum and all compounds are reported for the first time from this source.
Antioxidant Activity of Leaves of Calophyllum rubiginosum
American Journal of …
Problem statement: Calophyllum species have been used in traditional medicine for their therapeutic values for many years. This use was explained by several previous studies where antimicrobial, antifungal, anti-HIV and anti-cancer and antioxidant compounds were isolated from several Calophyllum species. Approach: The leaves were extracted with n-hexane (hexane), dichloromethane (DCM) and methanol using soxhlet apparatus. Total phenolic contents of each extract were evaluated using Folin Ciocalteau Reagent (FRC) while Ferric Thiocyanate (FTC) and 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) were used for lipid peroxidation and free radical scavenging activity respectively. Results: As the results, methanol extracts (5 mg mL −1 ) has higher phenolic contents of 66.2 µg mg −1 Gallic Acid Equivalent (GAE) compared to n-hexane and DCM extracts with phenolic content of 50.5 and 52.5 µg mg −1 GAE respectively. In FTC method, methanol had the lower absorbance values which indicate high antioxidant activities. In DPPH radical scavenging test however, DCM extract showed high antioxidant activity with an IC 50 value of 0.11mg mL −1 , followed by methanol and hexane with IC 50 values of 0.23 and 4.5 mg mL −1 respectively. The IC 50 of standards were 0.74 and 0.56 mg mL −1 for ascorbic acid and α-tocopherol, respectively. Conclusion: Finally, the positive outcome of the research can be further tested in the future to ascertain the active compound of the leaves of C. rubiginosum.
Antioxidant Activity of Leaves of Calophyllum rubiginosum
American Journal of Applied Sciences, 2010
Problem statement: Calophyllum species have been used in traditional medicine for their therapeutic values for many years. This use was explained by several previous studies where antimicrobial, antifungal, anti-HIV and anti-cancer and antioxidant compounds were isolated from several Calophyllum species. Approach: The leaves were extracted with n-hexane (hexane), dichloromethane (DCM) and methanol using soxhlet apparatus. Total phenolic contents of each extract were evaluated using Folin Ciocalteau Reagent (FRC) while Ferric Thiocyanate (FTC) and 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) were used for lipid peroxidation and free radical scavenging activity respectively. Results: As the results, methanol extracts (5 mg mL −1) has higher phenolic contents of 66.2 µg mg −1 Gallic Acid Equivalent (GAE) compared to n-hexane and DCM extracts with phenolic content of 50.5 and 52.5 µg mg −1 GAE respectively. In FTC method, methanol had the lower absorbance values which indicate high antioxidant activities. In DPPH radical scavenging test however, DCM extract showed high antioxidant activity with an IC 50 value of 0.11mg mL −1 , followed by methanol and hexane with IC 50 values of 0.23 and 4.5 mg mL −1 respectively. The IC 50 of standards were 0.74 and 0.56 mg mL −1 for ascorbic acid and α-tocopherol, respectively. Conclusion: Finally, the positive outcome of the research can be further tested in the future to ascertain the active compound of the leaves of C. rubiginosum.