Maturation of human acute myeloid leukaemia in vitro; the response to five recombinant haematopoietic factors in a serum-free system (original) (raw)

2008, British Journal of Haematology

CSF, G-CSF. M-CSF and Epo to induce maturation in human AML cells in vitro were investigated using cell specimens from 25 AML patients. The experiments were carried out under exactly defined serum-free culture conditions. In the absence of CSFs, monocytic and/or granulocytic maturation was detected in 14/25 cases. IL-3, GM-CSF. G-CSF and M-CSF elevated the proportions of monocyte/macrophages in 3/2 5, 2/25, 1/25 and 6/25 cases respectively, and increased the percentages ofmature granulocytes in 2/25, 1/25,1/25 and 0/25 cases, and if so only to a limited extent (values below 50%). The 'H-thymidine ('H-TdR) uptake studies revealed that IL-3, GM-CSF, G-CSF and M-CSF were efficient stiniulators of DNA synthesis of AML cells in 19, 15, 13 and four of those cases, respectively. Thus, although the cells in most Colony stimulating factors (CSFs) and erythropoietin (Epo) are known to regulate the expansion and cell lineage commitment of the haematopoietic progenitor cells. Multi-CSF (IL-3) and granulocyte-macrophage CSlF (GM-CSF) exert stimulative effects on the more primitive pi-ogenitors in the marrow, and granulocyte CSF (G-CSF), macrophage CSF (M-CSF) and Epo act particularly on maturation and functional activities of the more differentiated cells (Metcalf, 1984;