Maturation of human acute myeloid leukaemia in vitro; the response to five recombinant haematopoietic factors in a serum-free system (original) (raw)

2008, British Journal of Haematology

Abstract

CSF, G-CSF. M-CSF and Epo to induce maturation in human AML cells in vitro were investigated using cell specimens from 25 AML patients. The experiments were carried out under exactly defined serum-free culture conditions. In the absence of CSFs, monocytic and/or granulocytic maturation was detected in 14/25 cases. IL-3, GM-CSF. G-CSF and M-CSF elevated the proportions of monocyte/macrophages in 3/2 5, 2/25, 1/25 and 6/25 cases respectively, and increased the percentages ofmature granulocytes in 2/25, 1/25,1/25 and 0/25 cases, and if so only to a limited extent (values below 50%). The 'H-thymidine ('H-TdR) uptake studies revealed that IL-3, GM-CSF, G-CSF and M-CSF were efficient stiniulators of DNA synthesis of AML cells in 19, 15, 13 and four of those cases, respectively. Thus, although the cells in most Colony stimulating factors (CSFs) and erythropoietin (Epo) are known to regulate the expansion and cell lineage commitment of the haematopoietic progenitor cells. Multi-CSF (IL-3) and granulocyte-macrophage CSlF (GM-CSF) exert stimulative effects on the more primitive pi-ogenitors in the marrow, and granulocyte CSF (G-CSF), macrophage CSF (M-CSF) and Epo act particularly on maturation and functional activities of the more differentiated cells (Metcalf, 1984;

Loading...

Loading Preview

Sorry, preview is currently unavailable. You can download the paper by clicking the button above.

References (46)

  1. Abe, E., Miyaura. C.. Sakagami. H.. Takeda. M., Konno, K.. Yamazaki, T., Yoshiki. S. & Suda. T. (1981) Differentiation of mouse myeloid leukemia cells induced by 1,2 5-dihydroxyvitamin D3. Proceedings of the National Academy of Sciences of the United States of America. 78, 4990-1994.
  2. Beutler. B.. Greenwald, D.. Hulmes, J.D., Chang, M.. Pan, Y.-C.E., Mathison. J., Ulevitch, R. & Cerami, A. (1985) Identity of tumour necrosis factor and the macrophage-secreted factor cachectin. Nature, 316, 552-554.
  3. Boyum, A. (1968) Separation of leukocytes from blood and bone marrow. Scandinavian journal of Clinical and Laboratory Znvestiga- tion. 21, 77-89.
  4. Clark, S.C. & Kamen, R. (1 987) The human hematopoietic colony- stimulating factors. Science, 236, 1229-1 23 7.
  5. Collins, S.J.. Ruscetti. F.W.. Gallagher, R.E. & Gallo, R.C. (1978) Terminal differentiation of human promyelocytic leukemia cells induced by dimethyl-sulfoxide and other polar compounds. Pro- ceedings of the National Academy of Sciences of the United States of America, 75, 2458-2462.
  6. Delwel, R., Dorssers, L., Touw. I.. Wagemaker. G. & Lijwenberg. B. (1 98 7 ) Human recombinant multiiineage colony stimulating factor (Interleukin-3): stimulator of acute myelocytic leukemia progenitor cells in vitro. Blood, 70, 333-336.
  7. Delwel, R., Salem, M.. Pellens, C.. Dorssers, L.. Wagemaker. G.. Clark, S. & Lowenberg. B. (1988) Growth regulation of human acute myeloid leukemia: effects of five recombinant hematopoietic factors in a serum free culture system. Blood (in press).
  8. Dinarello, C.A. (1984) Interleukin-1 and the pathogenesis of the acute-phase response. New England fuurnal of Medicine, 31 1, 1413-1418.
  9. Fibbe. W.E.. Damme. J. van, Billiau, A.. Voogt. J.. Duinkerken. N.. Kluck, P.M.C. & Falkenburg. J.H.F. (1 986) Interleukin-1 (22-K factor) induces release of granulocyte-macrophage colony-stimu- lating activity from human mononuclear phagocytes. Blood, 68, French-American-British (FAB) Cooperative Group (1 9 76) Pro- posals for the classification of the acute leukaemias. British Journal of Haematology, 32, 451-458.
  10. French-American-British (FAB) Cooperative Group (1 98 5) Pro- posed revised criteria for the classification of acute myeloid leukemia. Annals OJ Znternal Medicine, 103, 626-629.
  11. Furukawa. Y.. Ohta. M.. Miura, Y. & Saito, N. (1986) Interleukin-1 producing ability of leukaemia cells and its relationship to morphological diagnosis. British Journal of Haematology, 65, 11-LS.
  12. Grabstein, K.H.. Urdal. D.L.. Tushinski. R.J., Mochizuki. D.Y.. Price, V.L., Cantrell. M.A.. Gillis. S. & Conlon, P.J. (1986) Induction of macrophage tumoridical activity by granulocyte-macrophage colony-stimulating factor. Science. 232, 506-508.
  13. Griffin, J.D., Young, D., Herrmann, F., Wiper, D.. Wagner, K. & Sabbath, K.D. (1986) Effects of recombinant human GM-CSF on 1316-1 321. the proliferation of clonogenic cells in acute myeloblastic leuke- mia. Blood, 67, 1448-1453.
  14. Hoang. T., Nara, N.. Wong. G.. Clark, S.. Minden. M.D. & McCulloch, E.A. (1986) Effects of recombinant GM-CSF on the blast cells of acute myeloblastic leukemia. Blood, 68, 3 13-316.
  15. Jacobs, K., Shoemaker. C., Rudersdorf. R.. Neill, S.D., Kaufman, R.J., Mufson, A., Sechra, J., Jones, S.S., Hewick, R., Fritsch, E.F.. Kawakita. M.. Shimizu, T. & Miyake, P. (1985) Isolation and characterization of genomic and cDNA clones of human erythro- poietin. Nature. 313, 806-810.
  16. Kawasaki, E.S.. Ladner, M.B., Wang, A.M., Arsdell, J. van, Warren, M.R.. Coyne. M.Y., Schweickart. V.L., Lee. M., Wilson, K.J., Bossman, A,, Stanley. E.R., Ralph, P. & Mark, D.F. (1985) Molecular cloning of a complementary DNA encoding human macrophage specific colony-stimulating factor (CSF-1). Science, Kelleher, C.. Miyauchi, J., Wong. G.. Clark, S., Minden, M.D. & McCulloch. E.A. (1987) Synergism between recombinant growth factors, GM-CSF and G-CSF acting on blast cells of acute myeloblas- tic leukemia. Blood, 69, 1498-1503.
  17. Koeffler, H.P.. Gasson. J., Ranyard, J., Souza, L.. Shepard, M. & Munker. R. (1 987) Recombinant human TNFalpha stimulates production of granulocyte colony-stimulating factor. Blood, 70, 55-59.
  18. Lin. F.K., Suggs. S., Lin. C.-H.. Browne. J.F.. Smalling. R., Egrie, J.C., Chen, K.K.. Fox, G.M.. Martin, F., Stabinsky. 2.. Baddrowi. S.M., Lai, P.-H. & Golowasser, E. (1985) Cloning and expression of the human erythropoietin gene. Proceedings ofthe National Academy of Sciences of the United States of America, 82, 7580-7584.
  19. Lotem. J. & Sachs, L. (1974) Different blocks in the differentiation of myeloid leukemic cells. Proceedings of the National Academy of Sciences of the United States of America. 71. 3507-3511.
  20. Lowenberg. B.. Hagemeyer. A. & Swart, K. (1982) Karyotypically distinct subpopulations in acute leukemia with specific growth requirements. Blood, 59, 641-645.
  21. Metcalf, D. (1984) The Hematopoietic Colony Stimulating Factors. Elsevier, Amsterdam.
  22. Metcalf, D. (1986) The molecular biology and functions of the granulocyte-macrophage colony stimulating factor. Blood. 67, Metcalf, D. & Nicola, N.A. (1985) Role of the colony stimulating factors in the emergence and suppression of myeloid leukemia populations. Molecular Biofogy ofTumor Cells (ed. by B. Wahren, G. Holm, S. Hammarstrom and P. Perlmann). pp. 21 5-232. Raven Press, New York.
  23. Miyauchi, J., Kelleher, C.A., Yang, Y.. Wong, G.G., Clark, S.C.. Minden, M.D., Minkin, S. & McCulloch. E.A. (1 987a) The effects of three recombinant growth factors, K-3, GM-CSF and G-CSF on the blast cells of acute myeloblastic leukemia maintained in short-term suspension culture. Blood, 70, 657-663.
  24. Miyauchi, J., Minden. M.D. & McCulloch, E.A. (1987b) The effect of recombinant human CSF-1 on acute myeloblastic leukemia (AML) cells in culture. Blood, 70 (Suppl.). 264a.
  25. Munker, R., Gasson, J.. Ogawa, M. & Koeffler. H.P. (1986) Recombi- nant human TNF induces production of granulocyte-monocyte colony-stimulating factor. Nature. 323, 79-82.
  26. Nagata. S., Tsuchiya. M.. Asano, S.. Kaziro. Y.. Yamazaki. T.. Yamamoto, O., Hirata, Y., Kubota, N., Oheda, M.. Nomura, H. & Ono, M. (1 986) Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor. Nature, 31 9, 41 5-418.
  27. Old, L.J. (1 987) Tumour necrosis factor: polypeptide mediator network, Nature. 326. 330-331.
  28. Perrussia. B.. Trinchicri, G., Lebman. D.. Jankiewicz. J.. Lange, B. & Rovera, G. (1 982) Monoclonal antibodies that detect differentia- tion surface antigens on human myelomonocytic cells. Blood, 59, 382-392. 230, 291-230. 257-267.
  29. Rambaldi, A.. Young, D.C. &Griffin, J.D. (1987) Expression of M-CSF (CSF-1) gene by human monocytes. Blood, 69, 1409-1413.
  30. Reyden, H.J. van der, Rhenen. D.J. van, Lansdorp, P.M., Veer, M.B. van 't. Langenhuijsen, M.M.A.C., Engelfriet, C.P. & Borne, A.E.G.Kr. von dem (1983) A comparison of surface marker analysis and FAB classification in acute myeloid leukemia. Blood, Sachs. L. (1986) Hematopoietic growth and differentiation factors and the reversibility of malignancy: cell differentiation and by- passing of genetic defects in leukemia. Medical Oncology and Tumor Pharmacotherapy. 3, 165-1 76.
  31. Salem. M., Delwel, R., Touw, I., Mahmoud, L. & Liiwenberg, B. (1988) Human AML colony growth in serum-free culture. Leukemia Research, 12, 157-165.
  32. Sieff. C.A. (1987a) Hematopoietic growth factors. Journal of Clinical Investigation. 79, 1549-1557.
  33. Sieff, C.A.. Tsai, S. & Faller, D.V. (1987b) Interleukin-1 induces cultured human endothelial cell production of granulocyte rnacro- phage colony-stimulating factor. Journal of Clinical Investigation.
  34. Suda, T., Sato, Y., Furakawa. Y., Suda. I., Mituoki, E., Saito, M. & Miura, Y. (1986) Myeloid and erythroid lineage expression of haematopoietic progenitors derived from an abnormal clone in erythroleukaemia. British Journal of Haematology, 64, 647-656.
  35. Thorens, B.. Mermod. J. & Vassalli. P. (1987) Phagocytosis and inflammatory stimuli induce GM-CSF mRNA in macrophages through posttranscriptional regulation. Cell, 48, 671-679.
  36. Touw, I. & Liiwenberg. B. (1985) Variable differentiation of human acute myeloid leukemia during colony formation in vitro: a membrane marker analysis with monoclonal antibodies. British Journal of Haematology, 59, 37-44.
  37. Vellenga, E.. Young, D.C.. Wagner, K., Wiper, D., Ostapovicz. D. & Griffin, J.D. (1987a) The effects of GM-CSF and G-CSF in promoting growth of clonogenic cells in acute myeloblastic leukemia. Blood, Vellenga. E., Ostapovicz, D.. O'Rourke, B. & Griffin, J.D. (1987b) Effects of recombinant IL-3. GM-CSF and G-CSF on proliferation of leukemic clonogenic cells in short-term and long-term cultures. Leukemia, 1, 584-589.
  38. Weisbart, R.H.. Golde, D.W. & Gasson, J.C. (1986) Biosynthetic human GM-CSF modulates the number and affinity of neutrophil f-Met-Leu-Phe receptors. Journal of Immunology, 137,3584-3587.
  39. Wong, G.G., Witek. J.S., Temple, P.A., Wilkens, K.M., Leary, A.C., Luxenberg. D.P.. Jones, S.S., Brown, E.L.. Kay, R.M.. Orr. E.C..
  40. Shoemaker, C.. Golde. D.W., Kaufman, R.J., Hewick. R.M., Wang, E.A. & Clark, S.C. (1985) Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins. Science, 228, 810-81 5.
  41. Yam, L.T.. Li, C.Y. & Crosby. W.H. (1971) Cytochemical identifica- tion of monocytes and granulocytes. American Journal of Clinical Pathology. 55, 283-290.
  42. Yang, Y.C.. Ciarletta, A.B.. Temple, P.A., Chung. M.P., Kovacic, S.. Witek-Gianotti. J.S.. Leary. A.C.. Kriz, R., Donahue, R.E., Wong.
  43. G.G. &Clark, S.C. (1986) Human IL3 (Multi-CSF): identification by expression cloning of a novel hematopoietic growth factor related to murine IL3. Cell. 47, 3-10.
  44. Young, D.C. & Griffin. J.D. (1986) Autocrine secretion of GM-CSF in acute myeloblastic leukemia. Blood. 68, 1 1 78-1 18 1.
  45. Young, D.C., Wagner. R. & Griffin. J.D. (1987) Constitutive ex- pression of the granulocyte-macrophage colony-stimulating factor gene in acute myeloblastic leukemia. Journal of Clinical Investiga- tion. 79, 100-106.
  46. Zucali. J.R.. Broxmeyer. H.E., Dinarello. C.H., Gross, M.A. & Weiner. R.S. (198 7) Regulation of early human hematopoietic (BFU-E and CFU-GEMM) progenitor cells in vitro by interleukin 1-induced fibroblast-conditioned medium. Blood. 69, 33-3 7. 61,443-448. 79,48-51. 69, 1771-1 776.