Prevalence and histopathology of Sarcocystosis in slaughtered carcasses in southeast Iran (original) (raw)
Related papers
2020
Sarcocystis is considered as a common zoonotic coccidian parasite that infects intermediate hosts orally through ingestion of contaminated graze or water with protozoa oocyte. The purpose of study is to demonstrate the incidence of Sarcocystis infection in imported cattle in Duhok abattoir and achieve the gold conventional method for muscular tissue cyst and bradyzoites detection. Muscular tissue samples have been collected from esophagus, heart and diaphragm of 150 cattle. From a total of 1350 inspected samples (diaphragm, esophagus and heart) from three different imported origin have been 94%, 92% and 41.3% samples infected respectively as well as significant differences (p <0.01) in the distribution of Sarcocystis infection among organs included and microscopic method used while no significant been found in terms of the animal origin source. Moreover, acid pepsin digestion method has shown high sensitivity in detection of Sarcocystis infection. The infection with Sarcocystis i...
Veterinary World, 2021
Background and Aim: Sarcocystosis is a zoonotic infection caused by various species of Sarcocystis organisms with a worldwide geographic distribution. This study investigated the presence of Sarcocystis organisms in cattle and sheep slaughtered at an abattoir in Sulaimani Province in North Iraq. Materials and Methods: A total of 130 muscle samples were collected during May, June, and July of 2020, including 80 samples from sheep and 50 samples from cattle. Samples were examined visually for macrosarcocysts. The peptic digestion method was used to analyze fresh muscle tissue samples for detecting microsarcocysts followed by microscopic examination. Furthermore, muscle samples were fixed and stained with hematoxylin and eosin for histopathological examination. Results: In the gross examination, macroscopic cysts were not detected in both cattle and sheep; hence, all the prevalence data were obtained through microscopic observation of muscle samples. The peptic digestion method revealed the presence of banana-shaped bradyzoites in 90% and 92.5% of slaughtered cattle and sheep muscle samples, respectively. Organ-wise prevalence revealed that 95% and 92% of esophageal samples of sheep and cattle contained Sarcocystis spp., respectively. Moreover, 90% and 88% of sheep and cattle diaphragms were respectively infected. Histopathological examination of tissue sections revealed two morphologically distinct types of microsarcocysts, including thin-walled and thick-walled, in both sheep and cattle. Conclusion: The suspected Sarcocystis spp. were Sarcocystis tenella and Sarcocystis arieticanis in sheep and Sarcocystis cruzi and Sarcocystis bovifelis or Sarcocystis hominis in cattle. Infective stages of different Sarcocystis spp. are widespread in the study area environment.
Sarcocystis infection in slaughtered cattle in Zango abattoir, Zaria, Nigeria
Veterinary World, 2013
Background: Sarcocystis infection is a parasitic zoonosis, which may cause acute and fatal clinical diseases in susceptible cattle. When raw or undercooked infected beef is consumed by man, it could result in intestinal sarcocystosis. Aim: This study aimed at determining the prevalence of Sarcocystis infection in slaughtered cattle in Zaria, Nigeria. Materials and Methods: A cross sectional study was conducted in which oesophagus and diaphragm samples were collected from 200 slaughtered cattle and analysed by pepsin-hydrochloric acid digestion and stained with Giemsa. Histological sections of tissues were prepared and stained with haematoxylin and eosin. Results: Eighty-five (42.5 %) were positive for Sarcocystis species. Sarcocysts ranged from 228.8 to 1215 µm in length and 46.93 to 114.40 µm in width. Sarcocysts were all microscopic in nature and 99.0 % had thin cyst wall (< 1 µm), while 4 % had thick cyst wall (3.61 to 7.22 µm). Sarcocystis cruzi and S. hominis were the identified species. Age, sex and breed were not determinants of the infection (p > 0.05). Seventy-five (88.2 %) and 56 (65.9 %) cattle had sarcocysts in the oesophagus and diaphragm respectively. There was a significant difference in the distribution of sarcocysts between the oesophagus and diaphragm (p < 0.05). Conclusion: This study has established in the study area the prevalence of Sarcocystis infection in cattle using tissue digestion method and histology. The identified species were of veterinary and public health importance.
The Prevalence of Infection of Sarcocystis Species in Cattle inZanjan Province, Northwest Iran
2020
Background: Sarcocystis is a protozoan parasite, capable of infecting many species of mammalian animals and birds worldwide. Transmission of some species to humans can happen through eating raw or under-cooked meats. Cattle as a major source of food production, harbor several species of Sarcocystis, including S. hominis, S. cruzi, S. hirsuta and one or two more species yet to be further validated. Little is known about the prevalence of Sarcocystis in livestock, especially cattle as a major human food source, in northwest of Iran.
Egyptian Journal of Veterinary Sciences, 2024
ARCOCYSTIS is an intracellular parasite belonging to the phylum Apicomplexa. More than two hundred species of Sarcocystis are capable of infecting different species of wild and domesticated animals and humans. This study aimed to determine the prevalence of Sarcocystis species and their morphological characteristics in the esophagi muscles of ruminants slaughtered at Zakho abattoir, Zakho City, Kurdistan Region/Iraq. Esophagi muscle specimens' of 1586 ruminants including, 764 sheep, 569 goats and 253 cattle were examined macroscopically and microscopically from April 2021to March 2022 to determine the prevalence of macroscopic and microscopic sarcocysts. Results revealed a total rate of infection with macrosarcocysts at 18.03 % (286 / 1586), with the highest rate 26.85% (254/946) in local breeds versus 5% (32/640) of imported ones. The highest rate among local breeds 90.91% (10/11) was in cattle, while the highest rate in imported breeds 9.13% (21/230) was among sheep. The overall rate in males was higher than in females. The detected macrosarcocysts relying on morphological characters were assigned as Sarcocystis gigantea, S. moulei, S. medusiformis, S. fusiformis, and S. hirsuta. Histological examination revealed the presence of microsarcocysts in the esophageal muscles of the three hosts which were assigned as S. tenella, S. arieticanis in sheep, S. capracanis in goats and S. bovifelis or S. hominis in cattle. The study results show the widespread of sarcocystosis among ruminants in this region that poses a threat to livestock and humans. This condition necessitates the development of preventive strategies to minimize or eradicate this parasitic infection.
Prevalence of Sarcocystis in slaughtered sheep carcasses in AL-bieda, Libya
Sarcocystis is an obligatory intracellular protozoan parasite, which can infect human and animals. Sheep are intermediate hosts for Sarcocysitis species. Despite the importance of worldwide sheep production, little is known about the prevalence of Sarcocystis in domestic sheep (Barbary breed) in Libya. The main aim of the present study is to determine the prevalence of Sarcocystis in sheep in Al-beida city, Libya, as well as to identify parasite based on macro and microcysts examination. Thirty five sheep (25 male and 10 female) slaughtered at local abattoirs in Al-beida city. Muscle samples were collected from heart, esophagus and diaphragm (35 each) of sheep carcasses and examined by digestion method igesting pepsin and Hcl for bradyzoites observation in the organs by light microscope. The results showed that, microcysts were more prevalent than macrocysts which were detected in only one esophagus sample in agedmore than one year age. The digestion technique revealed positive results in all samples taken from heart, diaphragm and esophagus (100%, 97% and 94%) respectively. There were significant differences between different ages, while the prevalence of Sarcocystis was not influenced by sex and seasonal variations. It could be concluded that prevalence of Sarcocystisis significantly high in Al-beida city.
Occurrence and Distribution of Sarcocystis Parasite Isolated from Sheep in Yazd Province, Iran
Introduction: Sarcocystis species are one of the most important meat borne parasites. Sarcocystosis causes several symptoms in human as well as numerous diseases with a high economic impact on livestock. In the current paper at first the results of a pilot study for determination of disease agent in the lambs of Yazd province in central Iran is presented. Then the status of parasite in red meat products in the study region is discussed. Materials and Methods: Muscles of 70 slaughtered sheep from both sexes and different ages were investigated for presence of parasite cysts from September to October 2013. Carcass inspection with naked eye at industrial slaughterhouses of Yazd for macroscopic cysts, and pepsin-digestion method for microscopic cysts was performed on common infected sites of infection. Results: No macroscopic cyst was seen at inspection. However, bradyzoites of parasite were observed in 97.14% of animals’ digested muscles. No significant difference between infection and age groups or sex of animals was observed. Conclusion: As humans are considered to be the final and intermediate host of different species of Sarcocystis and since parasite cysts are present at microscopic sizes, transmission of infection from lamb meat should come into consideration.
International journal of health sciences
This study aimed to determine and identify Sarcocystis spp. infection in sheep and goats in Misan governorate, Iraq. samples from oesophagus, diaphragm, tongue, and heart muscles were examined histologically for microscopic and macroscopic cyst , 70 out of 90 sheep 77.78 % had microscopic thick walled sarcocysts with mean 53 ×24.72 μm diagnosed as Sarcocystis tenella while 25 out of them 27.78 % had microscopic thin-walled sarcocysts with mean 49.45×33 μm diagnosed Sarcocystis arieticanis , esophagus showed the highest infection rate above all organs examined for thick – walled Sarcocystis while diaphragm was the highest for thin –walled Sarcocystis , the heart was the lowest infected organ for both type as 27.78% and 3.33% respectively . In goats 50 sample examined revealed 20 infected with thick walled sarcocysts only with different in distribution according to organs being high in esophagus 40% and lowest in heart 16% with mean measurement 60.61 × 37.75 μm diagnosed as Sarco...
Sarcocystis sinensis in slaughtered cattle from Central of Iran
Journal of the Hellenic Veterinary Medical Society
Sarcocystis is a food borne parasite as an intracellular parasite with two-host life cycle. Humans serve as either intermediate or definitive hosts among different species. Sarcocystosis is a very important disease because of its widespread prevalence with an impact on production and animal health. We aimed to assess S. sinensis in cattle from the desert area of Iran, using conventional PCR, targeting 18S rRNA. Also, the phylogenic analysis was done to mention isolate and the related ones in Genbank.From the 108 beef samples of heart, esophagus, intercostal muscle, diaphragm, and tongue harboring Sarcocystis spp. stored in the BioBank of the Research Center for Health and Food Safety, Shahid Sadoughi University of Medical Sciences, Yazd, Iran, DNA extraction was done. Amplification was done using the primer pair of SAR-F: 5'-TGGCTAATACATGCGCAAATA-3' and SAR-R: 5'-AACTTGAATGATCTATCGCCA-3'For the target of 18S rRNA gene. Sequencing was done. The interesting sequence wa...
Alexandria Journal of Veterinary Sciences, 2021
The importance of Sarcocystis emerged from the public health point of view, as it causes zoonotic infection. This study aimed to investigate the prevalence of Sarcocystis in economically important animals (cattle and buffalo) to lower the zoonotic potential of this parasite. A total of 7435 slaughtered animals (3879 cattle and 3556 buffalo) were examined macroscopically and microscopically for the detection of Sarcocystis followed by molecular detection and identification using PCR and Sequencing of 18S rRNA gene. The overall infection of macroscopic Sarcocystis was a higher prevalence in females 62.73 % than in males (0.73 %). In buffalo, the higher prevalence of macroscopic Sarcocystis observed in esophagus 46.82% tongue 9.3%, forequarter 3.9%, for buffalo female and male. Although the overall prevalence of microscopic cyst was 87%, the highest prevalence was in heart 63% (88% and 38%), esophagus 56% (72% and 40%), forequarter 55% (64% and 46%), tongue 41% (36% and 46%), diaphragm 32% (26% and 38%), hindquarter 27% (30% and 24%) in cattle and buffalo. Furthermore, the molecular identification showed that isolates Sarcocystis were S. cruzi, and S. hominis in cattle and buffalo, and S. levinei and S. sinensis in buffalo comparing with published data on GenBank. The present study is the first report identified S. hominis infection in Egyptian buffaloes in Menoufia Governorate and the second record of identifying S. cruzi in Egyptian buffaloes. Using PCR is a more sensitive method in the detection of different Sarcocystis spp. In Egypt. a greater hygienic application is required.