Cytotoxicity of hydro-alcoholic extracts of Cucurbita pepo and Solanum nigrum on HepG2 and CT26 cancer cell lines (original) (raw)
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CYTOTOXIC STUDIES ON SELECTED MEDICINAL PLANTS
European Journal of Biomedical and pharmaceutical sciences, 2016
Methanolic extract of flower bud of Syzygium aromaticum (L.) Merr. & L.M.Perry, leaves of Volkameria inermis L. (syn. Clerodendrum inerme L.) whole plant of Sphaeranthus amaranthoides Burm.f. and combination of these three drugs were studied cytotoxic activity against a human hepato cellular carcinoma cell line, Hep G2 in different concentrations (10, 20, 40 & 80μg/ml) along with standard drug Adriamycin (Doxorubicin)(ADA)(Positive control compound). The results showed that all the extracts were non-cytotoxic at the dose levels studied and all of them showed GI50 only at concentration > 80 μg/ml.
Cytotoxicity of Solanum nigrumL green fruits on breast (MCF-7) and liver (HepG-2) cancer cell lines
Medicinal plants have been used for decades for the treatment of different diseases. Solanum nigrumL is an important medicinal plant in many traditional health care systems. It has been used for the management of liver disorders, diabetes, infections, cancer, inflammations and peptic ulcer. In the present study, we screened the green unripe S. nigrumL fruits and leaves for their cytotoxic activity. The ethanolic extract of green fruits revealed a marked cytotoxicity against breast (MCF-7) and liver (HepG-2) cancer cell lines with IC50of 12.7 and 16.6 µg/mL, respectively. This activity is related to the high content of steroidal glycoalkaloids in S. nigrumL unripe green fruits.
Asian Pacific Journal of Cancer Prevention, 2020
Objective: The purpose of this research was to evaluate the cytotoxic, cell cycle arrest, and apoptotic induction activities of the fruit of S. nigrum L. ethanolic-70% extract against MCF-7 human breast cancer cell. Methods: S. nigrum L. ripe fruit was blended and macerated with ethanol 70% and the filtrate was evaporated. The semisolid extract was then analyzed phytochemically. Cytotoxic analysis was performed using MCF-7 cancer and Vero normal cell by MTT method and followed by apoptotic and cell cycle arrest analysis using flow cytometry. Results: The phytochemical analysis resulted that extract contained total phenolic and flavonoid compounds with the level of 1.545±0.080% and 0.212±0.002%, respectively. Glycitin was the highest level of isoflavone compound, namely, 375.0844 mg/100 g extract. The cytotoxic evaluation revealed that the extract exhibited a selectively toxic effect between cancer and normal cell. The extract inhibited MCF-7 proliferation with IC50 value about 40.77±4.86 μg/mL and conversely toward Vero cell at lower cytotoxic activity with an IC50 value of 298.96±27.28 μg/mL. Evaluation of MCF-7 cell cycles demonstrated that the extract arrested the cell cycle in the S phase and continued to the G2/M phase at the half of the IC50 value. The extract induced apoptotic of MCF-7 cell about 43.31% in which this activity was nearly the same with doxorubicin as a positive control (59.14%). However, solamargine was predicted as the most active anticancer compounds by a molecular docking study so that it was suggested to measure the level of this compound. Conclusion: It can be concluded that the fruit of S. nigrum L. ethanolic-70% extract demonstrated cytotoxic activity toward MCF-7 breast cancer cell and nontoxic on Vero normal cell. Solamargine was predicted as the most active anticancer compound. This extract had an opportunity to be developed as a potential anticancer agent to overcome breast cancer diseases.
Cytotoxic Effects of Bangladeshi Medicinal Plant Extracts
Evidence-based Complementary and Alternative Medicine, 2009
To investigate the cytotoxic effect of some Bangladeshi medicinal plant extracts, 16 Bangladeshi medicinal plants were successively extracted with n-hexane, dichloromethane, methanol and water. The methanolic and aqueous extracts were screened for cytotoxic activity against healthy mouse fibroblasts (NIH3T3) and three human cancer-cell lines (gastric: AGS; colon: HT-29; and breast: MDA-MB-435S) using the MTT assay. Two methanolic extracts (Hygrophila auriculata and Hibiscus tiliaceous) and one aqueous extract (Limnophila indica) showed no toxicity against healthy mouse fibroblasts, but selective cytotoxicity against breast cancer cells (IC 50 1.1-1.6 mg mL −1 ). Seven methanolic extracts from L. indica, Clerodendron inerme, Cynometra ramiflora, Xylocarpus moluccensis, Argemone mexicana, Ammannia baccifera and Acrostichum aureum and four aqueous extracts from Hygrophila auriculata, Bruguiera gymnorrhiza, X. moluccensis and Aegiceras corniculatum showed low toxicity (IC 50 > 2.5 mg mL −1 ) against mouse fibroblasts but selective cytotoxicity (IC 50 0.2-2.3 mg mL −1 ) against different cancer cell lines. The methanolic extract of Blumea lacera showed the highest cytotoxicity (IC 50 0.01-0.08 mg mL −1 ) against all tested cell lines among all extracts tested in this study. For some of the plants their traditional use as anticancer treatments correlates with the cytotoxic results, whereas for others so far unknown cytotoxic activities were identified.
In Vitro Screening for Cytotoxic Activity of Herbal Extracts
Evidence-Based Complementary and Alternative Medicine
Experimental studies have shown that a variety of chemopreventive plant components affect tumor initiation, promotion, and progression and the main difference, between botanical medicines and synthetic drugs, resides in the presence of complex metabolite mixtures shown by botanical medicine which in turn exert their action on different levels and via different mechanisms. In the present study, we performed an in vitro screening of ethanol extracts from commercial plants in order to investigate potential antitumor activity against human tumor cell lines. Experimental results obtained through a variety of methods and techniques indicated that extracts ofI. verum,G. glabra,R. Frangula, andL. usitatissimumpresent significant reduction inin vitrotumor cell proliferation, suggesting these extracts as possible chemotherapeutical adjuvants for different cancer treatments.
Cytotoxic Activity of Four Mexican Medicinal Plants
2009
Ibervillea sonorae Greene, Cucurbita ficifolia Bouché, Tagetes lucida Cav and Justicia spicigera Scheltdd are Mexican native plants used in the treatment of different illnesses. The ethanolic extract of J. spicigera and T. lucida as well as aqueous extracts from I. sonorae, C. ficifolia, T. lucida and J. spicigera were investigated using sulforhodamine B assay. These extracts were assessed using two cell line: T47D (Human Breast cancer) and HeLa (Human cervix cancer). Colchicine was used as the positive control. Data are presented as the dose that inhibited 50% control growth (ED 50 ). All of the assessed extracts were cytotoxic (ED 50 < 20μg/ml) against T47D cell line, meanwhile only the aqueous extract from T. lucida and the ethanolic extract from J. spicigera were cytotoxic to HeLa cell line. Ethanolic extract from J. spicigera presented the best cytotoxic effect. The cytotoxic activity of J. spicigera correlated with one of the popular uses, the treatment of cancer.
Cytotoxic Activities of Certain Medicinal Plants on Different Cancer Cell Lines
Turkish Journal of Pharmaceutical Sciences
Son yıllarda kanserin önlenmesi ve tedavisi için, farklı sınıfta fitokimyasal içerikleri ve daha az yan etkileri nedeniyle bitkilerin kullanımı oldukça önem kazanmaktadır. Bu çalışmada, Türkiye'nin Kars ilinden toplanan dört farklı tıbbi bitki türünün altı farklı kanser hücre hattı ve bir normal hücre hattı üzerindeki sitotoksik etkileri incelenmiştir. Gereç ve Yöntemler: Sitotoksik aktivite MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-dipenyltetrazolium bromide] yöntemiyle belirlenirken, apoptotik hücre ölümü akış sitometri ve kaspaz-3 analizleriyle tespit edilmiştir. Bulgular: Artemisia absinthium yaprak özütü A-549, CCC-221, K-562, MCF-7, PC-3 hücre hatları üzerinde anlamlı sitotoksik etki (≥%70) gösterirken, tohum özütü ise CCC-221, K-562, MCF-7, PC-3 hücreleri üzerinde anlamlı sitotoksik etki (≥%70) göstermiştir. Normal hücre hattı Beas-2B hücresine kıyasla, seçici sitotoksik aktivite yaprak özütüyle A-549 ve K-562 hücreleri üzerinde gözlenirken, tohum özütüyle K-562, MCF-7 ve PC-3 hücreleri üzerinde gözlenmiştir. Her iki özütün sitotoksisite seviyesi düşük konsantrasyonlarda zaman ve doza bağlı olarak değişmektedir. İlave olarak, Plantago major bitki özütüyle A-549 hücreleri üzerinde seçici sitotoksisite (%78) elde edilmiştir. Hyoscyamus niger ve Amaranthus retrosa özütlerinin sitotoksisite aktivitesinin %10 ve %30 olduğu gözlenmiştir. Sonuç: A. absinthium özütleri ve P. major tohum özütü gelecek çalışmalarda bazı kanser hücrelerinin sitotoksisitesi için tedavi edici ilaç geliştirilmesi potansiyeline sahiptirler.
Cytotoxicity Activity of Some Indian Medicinal Plants
International Journal of Current Pharmaceutical Research, 2016
Objective: The current study is carried out to evaluate cytotoxicity activity of the methanolic extracts of some medicinal (Buchanania axillaris Desr, Tamilnadia ulignosa Retz, Phaseolus semierectus L and Stylosanthes fruticosa Retz).Methods: Cytotoxicity activity was evaluated on human cancer cell lines such as lung cancer (A549) and skin cancer (A431) using MTT assay method.Results: The selected plant extracts showed the dose-dependent cytotoxicity activity on the tested cell lines. The cytotoxicity variations on different cell lines were also observed for tested plants extracts. The cytotoxicity of the extracts was increased as the concentration of them was increased. Among all tested plants extracts Phaseolus semierectus showed the better cytotoxicity activity on tested cell lines. Conclusion: The results of the present study supported the folkloric usage of the studied plants and confirmed that the plant's extracts have the bioactive constituents with cytotoxic properties a...