Identification and Evaluation of Cotton and Tomato Plant Volatiles as Attractants for Certain Lepidopterous Insect Pests (original) (raw)
Related papers
Entomologia Experimentalis et Applicata, 2019
Essential oils of aromatic plants and their individual volatile components have been tested in pest management strategies for their toxic and often repellent effects on target insects. When evaluating their possible effects on crucial behaviours of the pest insects, the olfactory environment including intraspecific communication cues has to be considered. We used the flour beetle Tribolium confusum du Val (Coleoptera: Tenebrionidae), a common stored-product pest, to investigate the influence of oil of spike lavender, Lavandula spica Medik. (Labiaceae), and its main component, linalool, at various doses on olfactory-guided behaviour. Using four-way olfactometers, a dose-dependent repellent effect of L. spica oil and linalool alone was revealed. On the other hand, we confirmed that T. confusum is attracted by conspecifics, by means of an aggregation pheromone and by 10 ng of one of its components, 1-tetradecene. Twenty-four hour pre-exposure to 10 ll of L. spica oil abolished subsequent attraction to 1-tetradecene and reduced attraction to five conspecifics. Simultaneous exposure to L. spica oil or linalool and five conspecifics reduced the repellent effect of the volatiles in a dose-dependent manner, whereas simultaneous exposure to 1-tetradecene at 10 ng abolished the repellent effect of L. spica oil only at a dose of 0.01 mg. These results indicate a dose-dependent trade-off between attractive and plant-derived repellent volatiles, which may influence the effectiveness of such volatiles in their potential use in alternative pest management strategies.
In the preliminary screening, 10 plant volatile oils have been tested for their repellent activity of against three important lepidopteran species such as Erias vitella, Helicoverpa armigera and Spodoptera litura. Lavender, rosemary, cedarleaf and basil oils showed less than 25% repellency, whereas, ginger and cornmin oils showed more than 25% activity. Besides lime, calamus, lemon and tagetes oil showed remarkable repellent activity (more than 50%) against E. vitella. Furthermore, lime, calamus, lemon and tagetes oils showed more than 50% repellent activity, while the trend was followed by ginger, lavender, rosemary, cedarleaf, basil and cornmint oils which showed less than 50% repellent activity against S. litura. It was apparent to note that, H. armigera is less susceptible to lavender, cedarleaf and basil oils which have been showed less than 25% activity. Calamus, lemon and tagetes oils showed pronounced ovipositional repellent activity followed by ginger lime and rosemary. Further the line of experiment was attempted with oil formulation, showed maximum percentage of ovipositional repellent activity against the gravid moths of H. armigera followed by S. litura and E. vitella were 84.75 ± 12.50 (66.97), 79.90 ± 6.75 (63.36) and 76.55 ±10.55 (61.00) respectively.
Industrial Crops and Products Volume 210, 2024
Aromatic plant essential oils (EOs) represent a promising alternative to synthetic compounds for humans, food and crop protection from insect pests. However, although many studies have demonstrated the toxic and repellent effect of EOs against harmful insects, some studies indicate that EOs can act, on the contrary, as attractants for harmful insects. In the practical use of EOs as active ingredients in insecticidal/repellent formulations, these contrasting effects may generate unexpected responses from insects. In this work, we tested the hypothesis that EOs may act in a hormesis biphasic concentration-response effect characterized by a lowconcentration stimulation (attraction) and a high-concentration inhibition (repulsion) of the insect chemotaxis. For this purpose, the EOs obtained from twelve aromatic plant species belonging to eight botanical families were chemically characterized and tested for their toxicity (by fumigation) and attractiveness or repellence (by two-choice olfactometer bioassays) against the stored cereals pest Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae). EO compositions were variable both qualitatively and quantitatively: while phenylpropanoids were the most represented chemical class in the EOs of Cinnamomum verum, Myristica fragrans, Ocimum basilicum, Piper aduncum, and Syzygium aromaticum, monoterpene hydrocarbons dominated the Citrus aurantium and Citrus reticulata EOs compositions, and they represented over 60% of the Schinus molle EO; the Clinopodium nubigenum and Clinopodium tomentosum EOs, as well as the Elettaria cardamomum EO, were mainly rich in oxygenated monoterpenes, while Piper nigrum was the only species among those studied whose EO composition was mainly represented by sesquiterpene hydrocarbons. Toxicity tests showed a wide range of activity among the tested EOs, with LC 50 values ranging from 2.19 to 218.32 µL L − 1 for C. nubigenum and C. verum EOs, respectively. Behavioral tests showed that C. aurantium, C. nubigenum, C. tomentosum, and O. basilicum EOs exerted an overall repellent effect, which was significant at concentrations ranging from 1.19 to 23.87 µL EO L − 1 air. Conversely, C. verum and S. aromaticum EOs were predominantly attractive, with a significant attractiveness in the range of concentrations from 2.39 to 11.94 and at 23.87 µL EO L − 1 air, respectively. However, half of the tested EOs (C. reticulata, E. cardamomum, M. fragrans, P. aduncum, P. nigrum, and S. molle) showed both negative and positive chemotaxis depending on the EO concentration. Overall, the results indicated that EOs can exert attractive and repellent activity depending on their chemical composition and concentration. Since EOs contain volatile substances whose concentration varies over time after the application, the implication of these results will be discussed in relation to their possible use as insect repellents for active packaging or as attractive lures for trapping.
International Journal of ChemTech Research
Background: Spodoptera littoralis is a highly destructive insect pest. Use of insecticides to control larvae has led to several problems and hazards such as development of resistance and residual effects. Purpose: Evaluate the impact of the essential oils (bulk and Nano phase) of Purslane; Mustard and Castor oil were tested for their impacts against larvae of Spodoptera littoralis. Methods: The tested essential oils were obtained by steam distillation of dried plants and Encapsulation of Nano particles is a method over which a chemical is slowly but efficiently released to the specific host for insect pests control. Results: The most impact oil was Purslane oil (bulk and Nano) then Mustard and the least one was Castor. The high concentration % mortality of larvae was 70.0, 40.2 & 15. 5 % and 90.4, 80.8 & 62.2 % in Purslane, Mustard and Castor (bulk and Nano phase), respectively. The number of laid eggs decreased with increasing tested oils concentrations. Conclusion: Purslane essential oil showed good effect against the S. littoralis larvae followed by Mustard and Castor as Bulk and as Nano.
The present investigation revealed that the essential oil obtained from Acorus calamus, Ocimum sanctum, Eucalyptus globules, Rosmarinus officinalis and Cymbopogon citrates. They were tested against the ten pairs of gravid moths Spodoptera litura. Among the five oils tested, Cymbopongan citratus oil showed greater performance in terms of oviposition deterrent activity as it is evident from the data. It was observed that C. citratus oil treated surfaces received 2, 23, 42.66 and 56.66 mean number of eggs at 1000, 500, 250 and 125ppm concentrations respectively. These results suggest that selected essential oil have the potential to be used as an ideal eco-friendly approach for the control of agricultural pest population.
In the preliminary screening, 20 plant essential oils have been tested for their antifeedant activity of against three important lepidopteran species such as Spodoptera litura,.Helicoverpa armigera and Achaea janata .Six essential showed remarkable antifeedant activity (more than 50%) against selected pest species. Antifeedant activities of six essential oils have been tested with different concentrations 125, 250,500 and 1000 ppm. The maximum antifeedant activity was observed at 125 ppm concentration of R. officinalis (12.78) S. litura, S. officinalis (9.11) H. armigera and O. sanctum (12.44) A. janata. Furthermore, 250 ppm concentration produced M. spicata (22.47) S. litura, S. officinalis (26.88) H. armigera and O. sanctum (25.45) A. janata. Similarly, 500 ppm concentration recorded M. spicata (69.48) S. litura, O. sanctum (68.55) H. armigera and R. officinalis (71.33) A. janata. The most significant antifeedant activity was observed at 1000 ppm concentration S. officinalis (85.56) S. litura, M. spicata (82.85) H. armigera and M. spicata (90.55) A. janata.
Asian Journal of Agriculture and Rural Development, 2021
In this research, the repellent activity of five Lamiaceae essential oils, Agastache rugosa, Elsholtzia blanda, Elsholtzia ciliata, Elsholtzia penduliflora, and Plectranthus ovatus, was evaluated against sweet potato weevil, Cylas formicarius using a two-choice test between the essential oil-treated filter paper and the control. E. blanda and A. rugosa essential oils have some sweet potato weevil attractant properties at low dose (<47.16 nl/cm2),while N,N-diethyl-3-methylbenzamide (DEET), P. ovatus, E. penduliflora, and E.ciliata essential oils have repellent properties at doses ranging from 15.72 nl/cm2 to 196.49 nl/cm2. The effect of type of essential oil and their dose were interactively associated with repellent efficacy. There was a increase in repellent efficacy as the dose increased for all essential oils. The repellent activities of P. ovatus essential oil and E. penduliflora were higher than the others, and the repellent effects of E. ciliata essential oil and DEET were ...
Frontiers in Ecology and Evolution, 2015
The insect olfactory system discriminates odor signals of different biological relevance, which drive innate behavior. Identification of stimuli that trigger upwind flight attraction toward host plants is a current challenge, and is essential in developing new, sustainable plant protection methods, and for furthering our understanding of plant-insect interactions. Using behavioral, analytical and electrophysiological studies, we here show that both females and males of the Egyptian cotton leafworm, Spodoptera littoralis (Lepidoptera, Noctuidae), use blends of volatile compounds to locate their host plant, cotton, Gossypium hirsutum (Malvales, Malvaceae). Female S. littoralis were engaged in upwind orientation flight in a wind tunnel when headspace collected from cotton plants was delivered through a piezoelectric sprayer. Although males took off toward cotton headspace significantly fewer males than females flew upwind toward the sprayed headspace. Subsequent assays with antennally active synthetic compounds revealed that a blend of nonanal, (Z)-3 hexenyl acetate, (E)-β-ocimene, and (R)-(+)-limonene was as attractive as cotton headspace to females and more attractive to males. Two compounds, 4,8-dimethyl-1,3(E),7-nonatriene (DMNT) and (R)-(−)-linalool, both known plant defense compounds may have reduced the flight attraction of both females and males; more moths were attracted to blends without these two compounds, however, other compounds such as benzaldehyde may also be behavioral antagonists. Our findings provide a platform for further investigations on host plant signals mediating innate behavior, and for the development of novel insect plant protection strategies against S. littoralis.