Phenotypic and Molecular Detection of Extended-spectrum beta-lactamase among Ceftazidime Resistant Pseudomonas aeruginosa Isolated from Wound Swab (original) (raw)
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Journal of Fatima Jinnah Medical University
Background: Pseudomonas aeruginosa (P. aeruginosa) is a ubiquitous gram-negative rod shaped bacteria and a significant nosocomial opportunistic pathogen. Current study aimed at the investigation and determination of extended-spectrum beta-lactamase (ESBL) positive P. aeruginosa from clinical samples. Materials and methods: A total of 150 catheter tubes, pus, blood, and sputum samples were collected from three different hospitals in the twin cities of Rawalpindi and Islamabad. The isolates were identified by using standard microbiology procedures. Antibiotic susceptibility of the isolates was done through the disc diffusion method as per the protocol given by CLSI guidelines. Phenotypic characterization of ESBL producers was performed by combination disc test (CDT), double disc synergy test (DDST) and through PCR. Results: A total of 77/218 isolates were identified as P. aeruginosa. Among them 47 were resistant to different drugs, while 28 were identified as multidrug resistant. They...
SSR Institute of International Journal of Life Sciences, 2024
Background: Pseudomonas aeruginosa is the most prevalent Gram-negative bacteria associated with nosocomial infections. It is therefore necessary to study its trend of antimicrobial resistance. Few therapeutic alternatives are available as Extended spectrum Beta lactamases (ESBL)-producing organisms exhibit co-resistance to several antibiotics' classes. To study's aim is to study the prevalence, ESBL production, and resistance pattern of P. aeruginosa isolates obtained from respiratory samples employing phenotypic methods. Methods: This study was conducted on 560 clinical respiratory samples from patients presented to the School of Excellence in Pulmonary Medicine, NSCB Medical College, Jabalpur, India. It was a prospective study conducted in the Department of Microbiology on 100 P. aeruginosa samples obtained from respiratory samples of both patient's in-patient department (IPD) and outpatient department (OPD). Result: Among the 560 samples subjected to identification and isolation of aerobic growth, 385 samples were positive for bacterial growth. Of these, 100 (26%) samples were positive for P. aeruginosa. Antibiotic sensitivity pattern was determined for all the isolated strains. P. aeruginosa isolates showed maximum resistance to ticarcillin/clavulanic acid (79%), aztreonam (78%), and ceftazidime (74%). However, P. aeruginosa strains showed maximum sensitivity to piperacillin/tazobactam (88%). The same isolates were also examined using the double disc synergy method for phenotypic characterization of ESBL against ceftazidime and clavulanic acid using the CLSI standards. About 49% of the resistant P. aeruginosa strains isolated from sputum samples were positive for ESBL. Conclusion: The current work strongly suggests further research on P. aeruginosa antibiotic-resistant strains for ESBL phenotypic characterization.
Yemeni journal for medical sciences, 2022
Introduction: Pseudomonas aeruginosa is an important pathogen causing healthcare-associated infections especially in immunocompromised patients. It poses a threat to public health due to its inherent resistance to various antimicrobial agents and its ability to acquire new resistance through multiple mechanisms. Infections due to extended spectrum β-lactamase (ESBL) producing isolates of P. aeruginosa continue to be a challenge for clinicians as these result in high mortality and morbidity due to antimicrobial resistance. The aim of this study was to determine the prevalence of Cefotaximase-Munich (CTX-M) producing strains of P. aeruginosa in Sharda hospital, Greater Noida. Methods: Strains of P. aeruginosa isolated from various clinical samples were subjected to phenotypic detection for ESBL production by disc combination method. Positive strains were then subjected to polymerase chain reaction (PCR) for detection of blaCTX-M gene. Results: Out of 166 isolates of P. aeruginosa, 54 (32.53%) were phenotypically confirmed to produce ESBL. Out of these 54 isolates, 39 (72.22%) were positive for blaCTX-M gene. Multidrug resistance was found in 70 (42.17%) isolates. Imipenem was the most effective drug with a sensitivity of 64.86% whereas aztreonam was found to be least effective with sensitivity of only 36.74%. Conclusion: Current study highlights the phenotypic and molecular characterization of CTX-M gene in P.aeruginosa in our hospital setup. With judicious use of antimicrobials and strict infection control practices, it might be possible to limit the effect of these drug destroying enzymes.
Iranian journal of basic medical sciences, 2012
The aim of this study was to investigate the presence of PER-1-type ESBLs in drug resistant Pseudomonas aeruginosa isolates. During one-year period (2008-2009), following isolation and identification of 56 P. aeruginosa, the E-test method was performed for determination of minimal inhibitory concentration of ceftazidim. The isolates that they had MIC≥16 µg/ml against ceftazidim were used for determination of ESBL-producing by combined disk test (CDT) and double disk synergy test (DDST) methods. Bla PER-1 gene was investigated by PCR. P. aeruginosa KOAS was used as positive control. Twenty-nine (51.78%) out of fifty six isolates had MIC≥16 µg/ml to ceftazidime, twenty two (75.86%) of them were ESBL producers. Some isolates (27.5%) contained bla PER-1 gene. PER-1-type ESBLs producing P.aeruginosa has not been reported previously in but there has been a rather high prevalence of it.
Prevalence of Extended-Spectrum β-Lactamases Genes in Clinical Isolates of Pseudomonas aeruginosa
Medical Laboratory Journal , 2018
Background and Objectives: Pseudomonas aeruginosa is an opportunistic pathogen resistant to various antibiotics. The aim of the present study was to study resistant patterns in clinical isolates of P. aeruginosa, classify them into pandrug resistance (PDR), extensive drug resistance (XDR) and multidrug resistance (MDR) groups, and identify extended-spectrum β-lactamase (ESBL)-positive isolates using the phenotypic and genotypic methods. Methods: This cross-sectional study was conducted on 161 P. aeruginosa isolates collected from the city of Isfahan, Iran. Antibiotic susceptibility tests were performed using 11 antimicrobial agents. ESBL-positive strains were identified using the phenotypic and genotypic methods. Results: The highest level of antibiotic resistance was observed against ceftazidime (77.64%). None of the isolates was resistant to polymyxin B. In the phenotypic method, 64 isolates (39.75%) were found as ESLB-positive, whereas 132 isolates (81.98%) were ESBL-positive in the genotypic method. The number of ESBLpositive isolates in the genotypic method was significantly higher than in the phenotypic method. The frequency of XDR and MDR isolates was 50.93% and 27.32%, respectively. None of the isolates was PDR. The frequency of the bla TEM gene was significantly higher than other genes (P<0.0001). Conclusion: It was revealed that the genotypic method was much more accurate in identifying ESBL-positive strains than the phenotypic method. Therefore, use of the molecular method may increase the chance of successful treatment with antibiotics of the β-lactam family.
Prevalence of extended-spectrum β-lactamase in Pseudomonas aeruginosa in tertiary care centre
2020
Introduction: Pseudomonas aeruginosa an opportunistic nosocomial pathogen, its increasing resistance to broad-spectrum beta-lactams, mediated by extended-spectrum betalactamase enzymes (ESBL), is problem worldwide. The present study was undertaken to determine the prevalence of ESBL-production among the clinical isolates of Pseudomonas aeruginosa. Materials and Methods: Various clinical specimens received in our laboratory were processed and Pseudomonas aeruginosa was identified as per standard microbiological procedure. All isolates were subjected for ESBL screening test. Potential ESBL producer was then subjected for ESBL Phenotypic confirmatory test –Disc Diffusion method. Antimicrobial susceptibility test was performed by Kirby – Bauer disc diffusion method on all confirmed isolates as per Clinical Laboratory Standard Institute (CLSI 2016) guidelines. Results: A total of 322 non duplicate isolates of Pseudomonas aeruginosa identified during the study period. Of these 26.09% (n =...
2021
Aim: The current research was performed with an aim to discover the prevalence of ESBL producing P. aeruginosa and also to provide as a direct for doctors administration subjects by executing suitable infection control events as well as inventing an efficient antibiotic policy. Materials and Methods: The current research was performed in the Department of Microbiology at Gujarat Adani Institute of Medical Science, Bhuj, Kutch, Gujarat over a period for one year. All 250 isolates of Pseudomonas aeruginosa acquired from different clinical samples established in microbiology laboratory from IPD & OPD were incorporated in the research. Different clinical specimens established in our laboratory were coursed and Pseudomonas aeruginosa was recognized as apiece normal microbiological method. All isolates were subjected for ESBL screening test. Antimicrobial susceptibility test was performed by Kirby. Results: Highest samples established from middle age group (3050). Out of 250 isolates, 177...
Archives of Pediatric Infectious Diseases, 2015
Background: Pseudomonas aeruginosa is considered as a leading cause of nosocomial infections. Burn and wound infections are mainly caused by multidrug-resistant P. aeruginosa isolates. Drug resistance frequently occurs among nosocomial isolates and can usually resist a myriad of antibiotics such as novel β-lactam antibiotics. Detection of multidrug-resistant isolates could assist better drug administration. Objectives: The aim of this study was to detect Extended Spectrum Beta-Lactamases (ESBL) positive wound isolates and the genes encoding bla VEB-1 ESBL among wound isolates of P. aeruginosa. Materials and Methods: A total of 89 wound isolates of P. aeruginosa were collected from patients (47% (n = 42) were male and 53% (n = 47) were female) at six Iranian hospitals between years 2009 and 2011. Antibiotic susceptibility and phenotypic ESBL production tests were conducted. The combined disk was used to determine ESBLs production. The bla VEB-1 gene was detected with the polymerase chain reaction (PCR). Results: The majority of the wound isolates were resistant to augmentin (90%, n = 80) and cefpodoxime (87.6%, n = 78). However, the majority was susceptible to imipenem and meropenem. Fifty-eight (42%) wound isolates were ESBL positive. The antibiotic resistance amongst ESBL positive isolates was relatively higher than ESBL negative isolates. Twenty-three (40%) ESBL-positive isolates amplified the bla VEB-1 gene. Conclusions: More than behalf of the wound isolates were ESBL positive, and the presence of bla VEB-1 was determined in less than half of these isolates. Fortunately, resistance to imipenem and meropenem was low.