Comparison of the salivary immunoglobulin concentration levels between children with early childhood caries and caries-free children (original) (raw)
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The journal of contemporary dental practice, 2018
The occurrence of dental caries has become quite a common phenomenon nowadays. The varying levels of salivary secretory immunoglobulin A (SIgA) usually determine the progression of caries. The present study was aimed to determine the correlation between SIgA and mutans-specific antigen SIgA in children having different caries status. Scanning electron microscopic analysis was also completed to correlate the results. This study comprised 60 subjects, who were divided into three groups depending on caries status. In all, saliva was collected to determine the level of SIgA and mutans-specific antigen SIgA using enzyme linked immunosorbent assay (ELISA). The World Health Organization (WHO) criteria and method were used to evaluate dental caries. Bradford reagent was used to evaluate the levels of protein in the antigen. Furthermore, 20 sections of enamel were randomly obtained to estimate the severity of caries development among groups. Categorical characteristics among all groups were ...
Journal of IMAB - Annual Proceeding (Scientific Papers), 2010
SIgA is the main type immunoglobulin in the mixed saliva and is considered as the main secretion factor of the adaptive immunity in the mouth. The purpose of the study is an evaluation of the SIgA quantities and the connection of those antibodies with dental caries of children with different diseases and conditions influencing the oral medium. The study was performed with 116 children with diabetes, asthma, orthodontic problems (removable braces) and healthy controls. The following methods were used: (1) ELISA with "Salivary secretory IgA KIT of Salimetrics LLC USA; was used for quantitative determination of IgA-S in the saliva ;(2) Evaluation of the liquid oral medium (test "Saliva check-GC") and dental status (DMF-T) of the children studied. The results obtained came to show that 2/3 of the healthy children and the children with diabetes have low values of c(<100 µg/ml). In the group of healthy children there are no high values of SIgA; Middle values of SIgA (100-300 µg/ml) are most frequently met in children with asthmatic disease; Oone half of the children with orthodontic apparatuses have high values of SIgA (>300 µg/ml); There is no dependence between secretory immunity and dental caries in children.
2020
Salivary secretory immunoglobulin A (SIgA) has immunological control over dental caries and presumably prevents the adherence of cariogenic microorganisms to the hard surfaces, relation between SIgA and dental caries still in controversy. This study aims to investigate the relationship of SIgA, salivary flow rate and pH with the occurrence of Early Childhood Caries (ECC). A total of 36 patients aged 2-5 years old were divided into 3 groups, according to their caries status (caries-free, ECC and Severe ECC). The subjects were selected based on inclusive and exclusive criteria needed. ECC and Severe ECC were diagnosed based on AAPD guidelines. Score of dmft was recorded. Unstimulated salivary flow rate and pH were measured. The samples were analyzed for determining the SIgA level. Mean value of IgA levels in ECC group (2404.6516 ng/mL ± 395.03208 ng/mL) was higher compared to other caries status group, although there was no significant relationship as the p-value is 0.404. Median valu...
IOP Conference Series: Earth and Environmental Science
Severe Early Childhood Caries (SECC) is a significant dental health problem in various countries. The caries pathology process is affected by saliva status. An important antimicrobial component in saliva is sIgA preventing the attachment of bacteria to enamel and lysozyme capability to act as an antibacterial by hydrolyzing bacterial polysaccharide walls. The aim of the study is to compare the sIgAlevel and salivary lysozyme between children with caries free and suffering from SECC, aged under 3 years. This study is an observational analytic with cross sectional design. Saliva samples were collected unstimulated from 68 children. Examination of sIgA level and salivary lysozyme used ELISA sandwich technique. Data were analysed using Mann-Whitney and Kruskal Wallis, while correlation test used Spearman. The results showed,there was no significant differencein sIgAlevel between caries-free children and SECC children (p > 0.05). It has been found that in caries-free children have a h...
The role of salivary sIgA as protection for dental caries activity in Indonesian children
Clinical, Cosmetic and Investigational Dentistry, 2019
The aim of this study is to asses the relationship between the level of sIgA and dental caries experience in healthy children who are 6-to 9-years-old from Indonesia. The case-control study is conducted to determine the protective role of salivary secretory immunoglobulin A (sIgA) levels in the stimulated whole saliva of dental caries-active and caries-free children. Methods: This research was done by stimulating the whole saliva which had been collected from 6-to 9-years-old children with the index def-t≥3 of 30 children as the caries-active children group and 30 children with def-t<3 as the low caries-active children group. Saliva samples were collected in sterile vials between 10 am-12 pm due to the circadian rhythm, which is at least one hour after last meal. 1,5 ml of collected salivary sample was centrifuged, then the supernatants was transferred to other tube and stored immediately to the laboratory at a temperature of-20°C. The estimation of sIgA concentration was done by using ELISA. The differences in the level of sIgA between the two groups with caries were analyzed using the t-test afterward. Results: The total salivary concentration of sIgA was statistically significantly higher in the low caries-active children group than in the caries-active children group. Conclusion: The total salivary concentration of sIgA was statistically and significantly higher in the low caries-active children group than caries-active children Group. There is a negative correlation between sIgA level and dental caries activity of 6 to 9-years-old children.
https://www.ijhsr.org/IJHSR\_Vol.7\_Issue.6\_June2017/IJHSR\_Abstract.020.html, 2017
Introduction: Dental caries is considered to be a multifactorial disease, and it affects 60-90% of school-aged children worldwide. Salivary IgA is considered the first line of defense against pathogenic bacteria and their secretions. Controversial conclusions have been reported regarding the relationship between salivary IgA and dental caries activity; some studies demonstrated high concentrations of salivary IgA in a lower caries activity. Other studies showed high levels of salivary IgA with an increase in caries activity. Some authors did not observe any correlation. Aim: The aim of the present study is to assess the relation between the level of salivary IgA and dental caries in healthy children living in Makkah city. Materials and Methods: The study included a total of 90 children of both genders, age range from 7- to 12-year-old. Dental caries was assessed. Stimulated whole salivary samples were collected after dental examination in the morning (from 9 to 11 A.M.) and Saliva flow rates of the subjects were measured. The samples were transported then stored in saliva collection tubes. The salivary IgA levels were measured using Human IgA ELISA kit. Results and Conclusion: There is a negative correlation between the increase in the mean levels of salivary IgA with the reduction in dental caries activity in children. This would reflect the salivary IgA protective mechanism against dental caries and streptococcus mutans in the whole saliva of low caries-active children.
2021
To analyze the level of sIgA and Histatin-5 expression in Early Childhood Caries (ECC) and caries-free children. Saliva was collected from 31 subjects of ECC and 29 caries-free (ages 9-32 months). The level expression of sIgA and Histatin 5 was analyzed by enzyme-linked immunosorbent assay. The distribution and frequency of subjects consist of boys 47% and girls 53% with the average of ages 1-12 month 21% and 13-24 month 79%.sIgA and Histatin 5 expressions differ from the month periods tested (1, 3, and 9 months) but do not show any significant difference from each examination period (1 with three months; p>0.05: 0.116), (1 with nine months; p > 0.05: 0.766), and (3 to 9 months; p>0.05: 0.398). The expression of sIgA and Histatin 5 of boys and girls were not significant ( p >0.05:0.550) and weak correlation (r = 0.079).The level expression of sIgA in the Caries-free higher than ECC (p<0.05). Whereas the expression of Histatin 5 in ECC higher than Caries-free (p<0.0...
Journal of Clinical Pediatric Dentistry, 2021
Objectives: The aim of the study was to assess the salivary IgA (immunoglobulin A) and alpha amylase levels in the unstimulated whole saliva samples of caries-free and caries-active children and correlate it with the caries status and age. Study design: The salivary IgA and amylase was investigated in 100 children in the range of 8–12 years divided in two groups, control group (DMFT and/or deft = 0) and study group (DMFT/deft score ≥5). The salivary IgA was measured using kit based on two-site sandwich enzyme immunoassay principle and amylase was estimated using the vitro amyl slides. Results :The mean salivary IgA and amylase levels in the saliva of the children in the control group was found to be significantly increased (p=.001 and p=.014 respectively) whereas the relationship between salivary IgA and amylase levels in the saliva of the children was found to be insignificant with the age (p=.392 and p=.306 respectively). Conclusions: The results indicated that salivary IgA and am...
Salivary Immunoglobulin Gene Expression in Patients with Caries
Open Access Macedonian Journal of Medical Sciences, 2017
BACKGROUND: Immunoglobulins mediate the host’s humoral immune response are expressed in saliva.AIM: To quantify the FcαR, FcγRIIB, and FcαμR gene expression in the saliva of Mexican patients with caries in mixed and permanent dentition.SUBJECTS AND METHODS: This was a comparative cross-sectional study. mRNA was isolated from 200 μL of saliva following the RNA III Tissue Fresh-frozen protocol of the MagNA Pure LC Instrument 2.0 (Roche Diagnostics GmbH, Nederland BV) and the FcαR, FcαµR and FcγRIIB were quantified through TaqMan Assays.RESULTS: One hundred individuals, 50 with mixed dentition and 50 with permanent dentition, were included in the study. Statistically, it was found a significant difference (p = 0.025) in the IgG (FcγRIIB) expression between the studied groups.CONCLUSION: Although we confirmed the existence of FcαR, FcγRIIB and FcαμR gene expression in saliva, only a significant difference in the expression of FcγRIIB between the mixed dentition and perm...
IOSR Journal of Dental and Medical Sciences, 2016
This study is aimed to : assess the level of total proteins in the saliva; to assess the level of the immunoglobulin's IgA and IgG in the saliva; to assess the antioxidant capacity of the saliva; to analyze the correlation of the dental status and the level of activity of the dental caries in every examined parameter separately. Materials and methods: For the realization of our goals weincluded 74 preschool children (35 female and 39 male), picked randomly, at the age from 4-6 years, from the elementary schools in Shtip which were divided into two groups: experimental and control. Every examinee was clinically examined (dmft and intensity of dental caries) and laboratory investigations were carries out (total protein levels, antioxidant capacity, IgA and IgG in the saliva). Results: The results from our study that referred to the total amount of proteins in the saliva of children with primary teeth, from the control group, were significantly greater in correlation to the value of the same in children from the experimental group (p<0,01); we established a negative medium strong significant correlation (p<0,05) between the values of the total protein in the saliva of children with primary teeth and the presence of dental caries; the values of the total antioxidant capacity of the saliva in children with a primary dentition from the control group are significantly greater in correlation to the values of the same in the saliva of children from the experimental group (p<0,001); The examined correlation between the total antioxidant capacity of the saliva in children with primary dentition and the presence of dental caries, showed that there is a negative medium strong significant correlation (p<0,05; the levels of IgA and IgG in the saliva in children from the control group with primary dentition is significantly greater in correlation to the values of IgA and IgG in the saliva in children from the experimental group (p<0,001); the correlation between the values of the immunoglobulin A and G in the saliva of children with primary dentition and the presence of dental caries, showed that there is a negative medium strong significant correlation (p<0,05).