Dna Cleavage and Antimicrobial Activity Studies on Transition Metal(II) Complexes of 4-AMINOANTIPYRINE Derivative (original) (raw)
Related papers
A novel series of transition metal complexes of Cu (II), Co (II), and Cr (III) have been synthesized from the Schiff base derived from Biginelli Product and 4-aminoantipyrine. In this study of Ligand and metal chelates were synthesized and they were characterized by analytical, magnetic, Infrared, Electronic, 1HNMR, 13 C NMR and mass spectral study. The antimicrobial screening of the ligand and its complexes have been extensively studied on bacteria like Vibrio spp., Pseudomonas aeroginosa, Vibrio parahaemolytics, Salmonella spp., Aeromonas spp., Klebsiella spp., Proteus spp. and fungi such as, Aspergillus flavus, Pencillium spp., Trichophyton. The DNA cleavage activity of the ligand and its metal complexes were studied by gel electrophoresis method. The MTT bioassay was also carried out to study the in cytotoxicity properties for the ligand and its metal complexes against VERO and Hep2 cell line. The results also indicate that the metal complexes are better antimicrobial agents as compared to the Parent Schiff base ligand and Biginelli product.
Journal of Chemical Sciences, 2007
A new series of transition metal complexes of Cu(II), Ni(II), Co(II), Mn(II), Zn(II), VO(IV), Hg(II) and Cd(II) have been synthesized from the Schiff base (L) derived from 4-aminoantipyrine, 3-hydroxy-4-nitrobenzaldehyde and o-phenylenediamine. Structural features were obtained from their elemental analyses, magnetic susceptibility, molar conductance, mass, IR, UV-Vis, 1 H NMR and ESR spectral studies. The data show that these complexes have composition of ML type. The UV-Vis, magnetic susceptibility and ESR spectral data of the complexes suggest a square-planar geometry around the central metal ion except VO(IV) complex which has square-pyramidal geometry. The redox behaviour of copper and vanadyl complexes was studied by cyclic voltammetry. Antimicrobial screening tests gave good results in the presence of metal ion in the ligand system. The nuclease activity of the above metal complexes shows that Cu, Ni and Co complexes cleave DNA through redox chemistry whereas other complexes are not effective.
A series of transition metal complexes of Co(II), Ni(II), Cu(II) and Zn(II) have been synthesized from their corresponding metal chlorides using a macrocyclic Schiff base as a ligand which is derived from benzil, 4-aminoantipyrine and o-phenylenediamine. Elemental analyses, magnetic susceptibility, molar conductance, Mass, FT-IR, Far IR, UV-Vis, VSM,1H NMR, 13C NMR,SEM, EDX and ESR have been used to assign the chemical structure of synthesized transition metal complexes. The UV-Vis, magnetic susceptibility and ESR the spectral data reveal that all complexes are found to have octahedral geometry. Antimicrobial screening tests indicate that the metal complex of Cu(II) has good antimicrobial activity than the other metal complexes. The nuclease activity of the above metal complexes shows that Co(II)complex has cleave DNA effectively.
The Schiff base complexes of Co(II), Ni(II) Cu(II) and Zn(II) have been synthesized from 4-pyridine carboxaldehyde with 4-aminopyridine. They have been characterized by elemental analysis, molar conductance, molecular weight determination, IR, XRD and SEM. The metal in the Schiff base complex is hexa coordinated which binds through pyridine nitrogen, azomethine nitrogen and nitrate units of metal salt used in the preparation. The antimicrobial and cleavage activity have also been studied for the synthesized compounds. The Schiff base and its metal complexes show a good activity against the bacteria Staphylococcus aureus, E.coli, Klebsiella, Pneumonia and fungi like Candida albicans, Apergillus niger and Pencillium sp. The antimicrobial results also indicate that the metal complexes are better antimicrobial agents as compared to the Schiff base.
New series of mononuclear Schiff base Cu(II), Co(II), Ni(II) and Mn(II) complexes have been synthesized with Schiff base derived from o- phenylenediamine, furfuraldehyde and 2,2′ bipyridyl. The chelating tendency of the metal complexes have been analysed and elucidated using analytical, spectral (IR, UV, 1H NMR, ESR) and magnetic studies. These data prove that the Schiff base complexes show the octahedral geometry. Schiff base and their metal complexes have been tested for their antibacterial activities. These complexes show adequate growth inhibitory activity against bacteria like Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Klebsilla pneumonia than the free ligands. These complexes were determined by the nucleolytic cleavage activities on pUC18 plasmid DNA using gel electrophoresis in the presence of H2O2 and the complexes are to be found to good in nuclease activity.
With the aim of exploring biological properties of coordination compounds, we report for the first time novel Cu(II), Ni(II), Co(II) and Mn(II) complexes with newly synthesized heterocyclic Schiff base ligands derived from 4-chloro phenylenediamine, furfuraldehydeand2, 2′ bipyridyl. These compoundswere characterized by a set of chemical and spectroscopic methods to study elemental analysis, electrical conductance, magnetic susceptibility and spectral techniques that include IR, UV–Visand EPR. Elemental analysis data are consistent with the proposed formula. IR spectra confirm the hexadentate nature and coordination of the Schiff base complex. The octahedral geometry ofcomplexesis suggested by UV–Vis spectra and magnetic moment data. Antibacterial activities of the synthesized compounds were tested in vitro against the sensitive organisms Staphylococcus aureus, Bacillus subtilis as Gram positive bacteria, Escherichia coli and Klebsilla pneumonia as Gram negative bacteria and the results were discussed. The results showed an effective antimicrobial activity of the metal ions after coordination with the ligands. The nucleolytic cleavage activities of the complexes were carried out using gel electrophoresis.
Journal of the Serbian Chemical Society, 2007
Three new copper complexes of mixed ligands derived from Schiff bases (condensation of p-aminoacetanilide and substituted benzaldehydes) with 1,10-phenanthroline have been synthesized and characterized by elemental analysis, IR, UV-Vis, magnetic moments, conductivity and electrochemical measurements. The spectral techniques suggest that all the copper complexes exhibit octahedral geometry. The low electrical conductance of the complexes supports their neutral nature. The monomeric nature of the complexes was assessed from their magnetic susceptibility values. The in vitro biological screening effects of the investigated compounds were tested against the bacteria Escherichia coli, Staphylococcus aureus, and Salmonella typhi and the fungi Rhizopus stolonifer and Candida albicans by the serial dilution method. A comparative study of the MIC values of the Schiff bases and their copper complexes indicates that the metal complexes exhibited higher antibacterial activity than the free liga...
Metal complexes of Mn(II), Fe(II), Co(II) and Cd(II) ions with Schiff base ligand 4-{(pyridin-2-ylimino)methyl}phenol derived from condensation of 2-amino pyridine with 4hydroxybenzaldehyde was prepared. The ligand and complexes were isolated from the reaction in the solid form and characterized by conductivity, magnetic moment, TLC, IR, UV-Visible, thermal analysis and some physical measurements. During complexation reaction with transition metal ions Schiff base act as a deprotonated tridentate ligand and IR spectra showed that N and O atoms are coordinated to the central metal atom. The observed values confirmed that the complexes have octahedral geometry. The Schiff base and its metal complexes have been found to have moderate to strong antibacterial activity.
Arabian Journal of …, 2011
A novel Schiff base ligand has been prepared by the condensation between butanedione monoxime with 3,3 0 -diaminobenzidine. The ligand and metal complexes have been characterized by elemental analysis, UV, IR, 1 H NMR, conductivity measurements, EPR and magnetic studies. The molar conductance studies of Cu(II), Ni(II), Co(II) and Mn(II) complexes showed non-electrolyte in nature. The ligand acts as dibasic with two N 4 -tetradentate sites and can coordinate with two metal ions to form binuclear complexes. The spectroscopic data of metal complexes indicated that the metal ions are complexed with azomethine nitrogen and oxyimino nitrogen atoms. The binuclear metal complexes exhibit octahedral arrangements. DNA binding properties of copper(II) metal complex have been investigated by electronic absorption spectroscopy. Results suggest that the copper(II) complex bind to DNA via an intercalation binding mode. The nucleolytic cleavage activities of the ligand and their complexes were assayed on CT-DNA using gel electrophoresis in the presence and absence of H 2 O 2 . The ligand showed increased nuclease activity when administered as copper complex and copper(II) complex behave as efficient chemical nucleases with hydrogen peroxide activation. The anti-microbial activities and thermal studies have also been studied. In anti-microbial activity all complexes showed good anti-microbial activity higher than ligand against gram positive, gram negative bacteria and fungi.
Mixed ligand complexes of metals (Fe(III), Co(II), Cu(II), Ni(II) and Zn(II)) with the Schiff bases of L1 and HL2 (L1 - obtained through the condensation of 4-aminoantipyrine with furfuraldehyde and HL2 – derived from 2-aminophenol and vanillin) were synthesized. They were characterized using analytical and spectral techniques. Cyclic voltammogram of complexes in DMSO solution at 300 K was recorded and their salient features were summarized. The X-band ESR spectrum of the copper complex in DMSO solution at 300 and 77 K was recorded. All the synthesized metal complexes were screened for their in vitro antimicrobial activity against of bacteria and fungi by disc diffusion method. Comparative study of inhibition values of the Schiff bases and their complexes indicate that the complexes exhibit higher antimicrobial activity than the free ligands. The interaction of metal complexes with CT-DNA was investigated by UV-Vis., cyclic voltammetry, viscosity and thermal denaturation studies. The nuclease activities of the complexes were assayed on pUC19 DNA using gel electrophoreses in the presence of H2O2. All the metal complexes cleave the pUC19 DNA in presence of H2O2. The solvatochromic behaviour of complexes was discussed using UV-Vis., spectrophotometry in various solvents. Superoxide dismutase activity of these complexes has also been studied.