The Hypolipidaemic Effects of the Partially Purified Hypoglycaemic Agent of Aqueous Leaf Extract of Albizzia chevalieri Harms (original) (raw)
Related papers
2009
Aqueous leaf extract of Albizzia chevalieri and its fractions have been reported to possess significant hypoglycaemic effect. In the current work the effects of aqueous leaf extract of Albizzia chevalieri and the column chromatographic fractions, of the extract with hypoglycaemic effect, on serum lipid profile of alloxan induced diabetic rats were studied. The aqueous extract was fractionated with organic solvents and the fractions further fractionated using column chromatography. The results indicated that the aqueous leaf extract caused significant (P<0.05) decrease in triacylglycerol (14 %), LDLcholesterol (16 %), VLDL-cholesterol (19 %) and atherogenic index (45 %). The hexane fraction of the aqueous extract which was reported to possess significant hypoglycaemic effect caused significant reduction in serum total cholesterol (57 %), triacylglycerol (29 %), LDL-cholesterol (75 %), VLDL-cholesterol (67 %) and atherogenic index (72 %) in the rats. The second elution fraction of the hexane fraction from the column, which is dark brown in colour and was earlier reported to contain the hypoglycaemic agent also reduced the serum levels of total cholesterol, triacylglycerol, LDL-cholesterol, VLDL-cholesterol and atherogenic index by 66, 54, 78, 80 and 76 % respectively. These results may be indicative of the fact that the aqueous leaf extract of Albizzia chevalieri, in addition to it hypoglycaemic activity, also possesses significant hypolipidaemic effect. It may be worthwhile therefore to study the nature and mechanism of action(s) of the agent(s) responsible for the observed effects.
Nigerian Journal of Basic and Applied Sciences, 2010
The research investigated the hypoglycaemic and hypolipidaemic effects of aqueous and organic solvents extracts of Albizzia chevalieri root in alloxan-induced diabetic rats. The aqueous extract was administered at 100, 200 and 300mg/kg body weight while the organic solvent fractions of the aqueous extract were administered at 100mg/kg body weight. The 300mg/kg body weight of the aqueous crude extract caused a 24% reduction of serum glucose level of the diabetic rats. The chloroform and hexane fractions caused 25 and 24% reduction of serum glucose level of the diabetic rats. The results were comparable to 28% reduction obtained for treatment with 3.57mg/kg body weight of chlorpropamide. The petroleum ether fraction decreased serum total cholesterol, triaclglyceride, and very low density lipoprotein cholesterol levels significantly (P<0.05). chloroform fraction and last water extract fractions increased the high density lipoprotein cholesterol level of the diabetic rats. The results demonstrate that the aqueous root extracts of Albizzia chevalieri posses significant (P<0.05) hypoglycaemic and hypolipidemic effects in alloxan induced diabetic rats. The extract may be a potential source of lead compound(s) with anti-diabetic properties.
Scientific Journal of Medical Science, 2013
The study investigated the hypolipidemic and hepato-protective effects of n-butanol fraction of Alchornea cordifolia leaf extract in streptozotocin-induced diabetic rats. To achieve this set objective, 16 hours fasted rats were made diabetic by single intraperitoneal injection of 60 mg/kg body weight dose of streptozotocin dissolved in 0.1 ml fresh cold citrate buffer pH 4.5. After this, the diabetic animals were randomly divided into the following groups: Group I served as the normal control, Group II served as diabetic control, while Group III to Group VI were treated with 200, 400 and 800mg/kg b w of the plant extract fraction and glibenclamide 10mg/kg b w respectively by oral gavage for a period of 4weeks. At the end of treatment period all animals from each group were euthanized and blood samples collected by cardiac puncture. There was a statistically significant (p< 0.05) reduction in blood glucose level in all groups treated with 200, 400 and 800mg/kg b w of the extract after day 7, 14, 21 and 28 when compared to the diabetic control group. The study also revealed a significantly decreased (p<0.05) serum total cholesterol, triglyceride and low-density lipoprotein and significantly elevated (p<0.05) serum levels especially in the groups treated with 400 and 800 mg/kg b w of the extract when compared to the diabetic control group. There was also a significantly reduced(p<0.05) serum liver enzymes, AST, ALT and ALP as well as total and direct conjugated bilirubin levels in all groups that received various doses of the plant extract when compared to the diabetic control group. It can be concluded that the plant possesses anti-diabetic property suggesting that the plant maybe useful in the management of dyslipidemia, a secondary complication that usually occur in diabetic condition.
Asian Journal of Research in Biochemistry
Diabetes mellitus is a complex metabolic disorder associated with the development of metabolic complications. This research evaluated the hypoglycemic and hypolipidaemic effects of Moringa oleifera (MO), Treculia africana (TA) and Albizzia chevalieri (AC) plant extracts on diabetes. Albino rats were randomly divided into six (six) main groups; MC, MO, TA, AC, Normal Control (NC) and Diabetic Control (DC) groups. Group MO, TA and AC were further subdivide into three sub groups. Diabetes mellitus was induced by a single dose intraperitoneal injection of alloxan 150 mg/kg body weight. Fasting blood glucose level and lipid profile were assayed using standard methods. Intraperitoneal injection of 150 mg/kg of Alloxan in the albino rats resulted in significant (p<0.05) elevation of serum glucose, total cholesterol (TC), triglyceride (TG), very low density lipoprotein (VLDL-C), and low density lipoprotein (LDL-C). Also, there was significant decrease (p<0.05) in HDL-C and body weight...
Toxicity Studies of the Crude Aqueous Root Extract of Albizzia chevalieri Harms in Albino Rats
Nigerian Journal of Basic and Applied Sciences, 2011
Aqueous root extract of Albizzia chevalieri has been reported to possess hypoglycaemic and hypolipidaemic effects. The current work investigated the LD 50 of the crude aqueous root extract of Albizzia chevalieri and the effect of sub-chronic doses of the extract on liver and kidney function parameters of albino rats. The results indicated LD 50 greater than 3000mg/kg body weight in albino rats observed for 72 hours. The result of the sub-chronic toxicity on liver function parameters showed significant (P<0.05) increase in serum total protein and globulin at doses greater than 2000mg//kg, which was reflected on the A:G ratio. Transaminases and alkaline phosphatase activities were also significantly (P<0.05) affected at doses greater than 2000mg/kg. Serum levels of urea were also increased significantly (P<0.05) but creatinine and uric acids were decreased significantly (P<0.05) at doses greater than 2000 mg/kg., Serum electrolytes were however not affected. The extract should therefore be use with care especially at doses greater than 2000 mg/kg.
European Journal of Medicinal Plants
Background: Diabetes Mellitus is a debilitating metabolic disease characterized by hyperglycemia due to insufficient insulin or inability of insulin receptors to respond positively to circulating insulin. Prevalence of the disease is on the global increase annually with tendency to rise from 380 to over 592 million by 2035 in developing countries. Toxicity of some Orthodox drugs in use has led to searches for alternative sources for treatment and management of the disease. Aim: This work was aimed at investigating the effect of methanolic extract of Albizia lebbeck leaf on Alloxan monohydrate induced diabetic albino rats. Materials and Methods: Qualitative Phytochemical analysis of the plant extract was carried out in Biochemistry Division of National Veterinary Research Institute Vom, Plateau State, Nigeria. Forty female Wistar albino rats were used for the study, divided into five groups (A-E) in two replicates. Group A- negative control (non-induced, not treated) and group B- pos...
Asian Pacific journal of tropical medicine, 2011
To evaluate the antidiabetic potential of methanolic extract of Albizia odoratissima Benth. bark in alloxan induced diabetic mice. Group-I (normal control) mice received only basal diet without any treatment. In Group- II (Diabetic control) mice, diabetes was induced by alloxan (150 mg/kg i.p.) and received only Tween 80, 5% v/v in normal saline. Group- III and Group- IV mice received metformin (10mg/kg) and gliclazide (10 mg/kg) as standard drugs. Group- V and VI mice received methanolic bark extract of Albizia odoratissimaat doses of 250 and 500 mg/kg body weight p.o., respectively. The results of the study indicates that Albizia odoratissima bark extract significantly…
Toxicity Studies of the Crude Aqueous Root Extract of Albizzia chevalieri Harms in Albino Rats
Nigerian Journal of Basic and Applied Sciences, 2011
Aqueous root extract of Albizzia chevalieri has been reported to possess hypoglycaemic and hypolipidaemic effects. The current work investigated the LD 50 of the crude aqueous root extract of Albizzia chevalieri and the effect of sub-chronic doses of the extract on liver and kidney function parameters of albino rats. The results indicated LD 50 greater than 3000mg/kg body weight in albino rats observed for 72 hours. The result of the sub-chronic toxicity on liver function parameters showed significant (P<0.05) increase in serum total protein and globulin at doses greater than 2000mg//kg, which was reflected on the A:G ratio. Transaminases and alkaline phosphatase activities were also significantly (P<0.05) affected at doses greater than 2000mg/kg. Serum levels of urea were also increased significantly (P<0.05) but creatinine and uric acids were decreased significantly (P<0.05) at doses greater than 2000 mg/kg., Serum electrolytes were however not affected. The extract should therefore be use with care especially at doses greater than 2000 mg/kg.