Total lipid in the broodstock diet did not affect fatty acid composition and quality of eggs of sea bass (Dicentrarchus labrax L.) (original) (raw)
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Scientia Marina, 2001
To determine whether an increase in the quantity of lipids ingested by sea bass (Dicentrarchus labrax) broodstock could improve egg quality, three year-old sea bass were fed three different diets: a natural diet (Control group), and two artificial diets containing 10% or 17% of total lipids. In two consecutive reproductive seasons, the spawning results, the lipid classes and the fatty acid composition of the eggs were studied. No differences in the absolute content of lipids or in the percentage of different lipid classes were observed between the eggs from the three groups. Both experimental groups fed with the artificial diets produced eggs of poor quality, with low percentages of buoyancy and hatching. Those observed in the eggs from the Control Group were significantly higher (p>0.01) than those of the experimental groups. The higher hatching rate of the eggs from the Control Group was associated with higher DHA:EPA and AA:EPA ratios. The data obtained showed that the fatty acid composition of the eggs was affected by the fatty acid composition of the diets but not by the total quantity of lipids administered to the broodstock.
Aquaculture, 1997
Samples of sea bass (Dicetztrarchus labraxi eggs from broodstock which had been fed either a formulated pelleted feed, containing fish and corn oil, or a local trash fish, bogue (Boops boopsl were analysed for lipid class compositions, fatty acid compositions of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI) and wax ester and fatty alcohol compositions of wax esters. The pelleted feed contained 1.3 mg g -' of arachidonic acid (20:4n-6; AA) and an AA/eicosapentaenoic acid (20:5n-3; EPA) ratio of 0.1 while the trash fish contained 4.8 mg g-' AA and an AA/EPA ratio of 0.7. Docosahexaenoic acid (22:6n-3; DHA) concentrations were similar for both diets (about 23 mg g-' ). The fatty acid compositions of PC, PE and PI from eggs of fish fed trash fish contained significantly more AA. 22:5n-6 and DHA compared to fish fed the pelleted feed. The AA/EPA ratios in these phospholipids were around five-fold higher in the trash fish-fed group compared to those fed the fish and corn oil containing diet. In PI, which contains characteristically high levels of AA, the AA/EPA ratios were 1.5 and 8.6 for eggs derived from broodstock fed the pelleted diet and the trash fish, respectively. Determination of lipid class compositions of sea bass eggs revealed the presence of high levels of wax esters which were previously unrecorded in this species. The use of broodstock diets containing blends
Aquaculture Research, 2009
Adult black sea bass Centropristis striata broodstock (N 5162) were fed three di¡erent dietary treatments: two commercially prepared diets with 45% protein and two di¡erent lipid levels (12% and 20%) (diets 1 and 2), and a diet of frozen Atlantic silversides Menidia menidia (SS, diet 3). Broodstock were held under controlled photothermal conditions and induced to spawn with an LHRHa pellet (72 mg kg À 1 bw). Dietary lipid had pronounced e¡ects on spawning performance and egg quality. Diet 3 (SS) produced a signi¢cantly (Po0.05) higher fertilization success (22.4%) than diets 1 (0.6%) and 2 (4.8%). The hatching success of fertilized eggs was similar in all diets (range 5 40^58.6%), but only two spawns from diet 1 (12% lipid) yielded viable yolk-sac larvae (YSL). Diet 3 (SS) also produced signi¢cantly more YSL per female (21.8 Â 10 3 ) than the diet 1 (0.3 Â 10 3 ). Eggs from diet 3 (SS) contained a signi¢cantly greater proportion of n-3 series fatty acids, with docosahexaenoic acid (DHA) as the largest fraction. Eggs from commercially prepared dietary treatments contained signi¢cantly more n-6 fatty acids. The poor spawning performance of ¢sh fed diet 1 (12% lipid) may be related to higher levels of linoleic acid and lower levels of DHA in the diet.
Fish Physiology and Biochemistry, 1998
A feeding experiment was conducted on gilthead seabream (Sparus aurata) broodstock to investigate the incidence of n-3 highly unsaturated fatty acids (n-3 HUFA) dietary deficiencies on the lipid composition of female liver, gonads and eggs, in relation to spawning quality. Broodstock were fed a control (C) diet or a n-3 HUFA deficient (D) but linolenic acid rich diet. After 20 weeks of feeding, the results showed that levels of total neutral (TNL) and total polar (TPL) lipids of female gonads and eggs were independent of diet. However the fatty acid composition of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI) of female liver, gonads and eggs in the two groups of fish showed marked differences, reflecting the influence of fatty acid levels in the broodstock diets. This influence was even higher in TNL than in the phospholipid classes examined. In fish fed n-3 HUFA deficient diet, fatty acid composition of TNL of female gonads and eggs reflected the diet more than liver. A higher egg production in broodstock fed C diet (1.8% n-3 HUFA in diet) was extended to spawning quality such as percentages of fertilised and hatched eggs.
Aquaculture Research, 2009
The e¡ects of dietary n-3 highly unsaturated fatty acid (n-3 HUFA) on eggs and larval quality were investigated in the Chilean £ounder Paralichthys adspersus. Broodstock were fed with three formulated diets with similar proximate compositions but di¡erent n-3 HUFA (2.1%, 3.1% or 4.1%) estimated levels from 5 months before and during the spawning period. The diet with an intermediate n-3 HUFA level resulted in a signi¢cantly higher (Po0.05) percentage of buoyant eggs (68.2 AE 2.9%), fertilization (92.8 AE 3.9%), normal cell cleavages (93.5 AE 1.9%), hatching rate (87.7 AE 4.1%) and normal larvae (76.3 AE 3.7%) compared with the other two diets. In contrast, high levels of n-3 HUFA produced larvae with a higher survival capacity when subjected to fasting. The diet with the lowest content of n-3 HUFA produces lower quality eggs and larvae. The n-3 HUFA level in eggs increased with an increase in the dietary level, and the n-3/n-6 ratios were1:1, 2:1and 3:1. The DHA/EPA and EPA/ARA ratios of 2 and 4 in eggs, respectively, were associated with improved egg and larval quality and were similar to the ratios found in eggs from wild broodstock. Attainment of optimal fatty acid contents in broodstock diets is one of the key factors for producing the high-quality spawning required for managed culture of this £ounder.