Accumulation of Heavy Metals by Living and Dead Bacteria as Biosorbents: Isolated from Waste Soil (original) (raw)

In the present study Enterococcus luteus, Escherichia coli and Staphylococcus aureus have beenused for biosorption of cadmium and chromium from aqueous solution of various concentrations. Bacteriawere isolated from waste soil and identified through various morphological features, biochemical tests,and staining procedure. Biosorption capacity (both dead and live biomass) was observed through brothtechnique and absorbance values were measured using atomic absorbance spectrophotometer. Differentparameters were optimised for metal biosorption, including incubation periods (24, 48, 72 and 96 h) andpH (4, 6, 7, 8, 9, and 10) at 37 °C. Agar well and agar disc diffusion methods were used for resistogramand antibiogram analysis. Through agar well diffusion method, S. aureus showed complete resistanceagainst all concentrations of chromium and cadmium (50 to 300 µg/mL). E. luteus showed resistance on50 µg/mL and 100 µg/mL of both metals while E. coli exhibited resistance against all cadmium co...

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