Chemical alteration of the rhizosphere of the mycorrhizal-colonized wheat root (original) (raw)
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Acta Agriculturae Scandinavica, Section B - Plant Soil Science, 2006
Since most of the Central Anatolian soils are P and Zn deficient, mycorrhizae may help plants to obtain sufficient nutrients from the soil without the need to apply additional chemical fertilizers. As far as is known, some plants are strongly mycorrhizal dependent for P nutrition, but less is known about the mycorrhizal dependence with Zn nutrition. Hypotheses were tested under P and Zn deficient soils to find out whether kidney bean plants are mycorrhizal dependent or not. Kidney bean (Phaseolus vulgaris L.) plants were grown for 8 weeks in two widely distributed calcareous clay soils with low nutrient content from Central Anatolian Sultanö nü and Konya soils (sterilized by autoclaving). The experiment was conducted with three levels of phosphorus (0, 25, 125 mg P kg (1 soil), and two rates of Zn (0 and 5 mg Zn kg (1 soil) Two selected arbuscular mycorrhizal (AM) species (Glomus mosseae and G. etunicatum) were inoculated. In the Sultanö nü soil, mycorrhizal inoculation increased plant growth and P and Zn uptake. The positive effect of mycorrhizal inoculation on plant P content and uptake was found to be higher when higher levels of phosphorus were applied. The soil from Konya with a high concentration of boron inhibited normal growth of kidney bean plants. Mycorrhizal root colonization was different with mycorrhizal inoculation. Root colonization was not affected by P and Zn application, but it has been shown that the plant is strongly dependent on P nutrition, especially at low P application levels. However, although mycorrhizal inoculation increased plant concentration of Zn, plants were less dependent on Zn nutrition.
An experiment was conducted in pots containing field soil low in available P (7.8 mg P/kg soil) to evaluate the response of wheat (Triticunl aestivunz L. emend. Fiori & Paol.) genotypes to inoculation with arbuscular-mycorrhiza (AM) and its effect on phosphorus-utilization efficiency (PUE). The genotypes were inoculated with or without arbuscular-nlycorrhiza fungi (Gio1nus macrocarpUln Tul & Tul) in the absence ofP application to soil. Significant increase was observed in per cent root colonization (up to 50%), shoot (18-88%), root (25-960/0) and whole plant dry matter (29-91°1'0) under +AM inoculation, whereas the shootroot ratio was high under -AM. Phosphorus uptake in shoot was significantly higher due to +AM treatment in 'DL 1010-5' (39%) and 'DL 2044-1' (60%). Similarly, genotypes also showed a wide range of response to +AM under P stress. Genotype 'DLIOIO-5' recorded highest (191 %) degree of dependency on mycorrhiza and also produced nlaximunl number oftillers (5.67), shoot biornass (4.68 glplant), total plant dry matter (6.52 g/plant) and had high amount ofP uptake (10.17 mg/plant). The genotypes exhibiting a positive response to +AM such as more number oftillers, increased dry matter, higher root colonization, increased uptake ofnutrients and higher efficiency ofP utilization under P stress would provide the breeders with gene pool to develop genotypes with efficient Puptake and utilization abilities.
Asian Journal of Plant Sciences, 2004
Greenhouse pot experiment was conducted to evaluate the response of wheat grown in calcareous soil to arbuscular mycorrhizal fungi (AMF) and P application. The treatments consisted of two factors, five rates of P application (0, 50, 100, 200, 400 kg P ha-I) and two levels of AMF inoculation (inoculated and non inoculated). Wheat was grown for 7 weeks and at harvest wheat dry matter (DM), P and micronutrients uptake were determined. Roots were extracted and root length and surface area were measured. Roots also were stained in trypan blue and mycorrhizal colonization was determined. AMF inoculation increased mycorrhizal colonization which decreased at higher rates of P levels. Maintaining the maximum DM while the AMF colonization was depressed at higher P rate indicates that the increase in DM was solely due to P. AMF inoculation decreased root length and surface area at higher rates of P. Phosphorus uptake was higher for mycorrhizal plants at 50 and 100 kg ha-I P rates only. Linear and quadratic relationship between P uptake and the root surface area were observed for nonmycorrhizal and mycorrhizal wheat, respectively. Higher specific P uptake and higher physiological and agronomical use efficiencies at lower P rates by mycorrhizal wheat suggests that they are more efficient in P absorption and utilization of soil P that is unavailable to nonmycorrhizal wheat AMF enhanced Fe, Zn and Cu uptake except at high P rates where both mycorrhizal and nonmycorrhizal wheat had similar values of copper uptake.
Communications in Soil Science and Plant Analysis, 2018
The effect of indigenous soil and selected mycorrhizal inoculation and phosphorus (P) applications on wheat yield, root infection and nutrient uptake was monitored for two successive years under field conditions. In addition, phosphorus efficiency and inoculation effectiveness (IE) were determined. Wheat (Triticum aestivum L.) plants were used as host plants in a Menzilat soil series (Typic Xerofluvents) in the Mediterranean coastal region of Turkey. Three levels of phosphorus were applied with Glomus mosseae to wheat plants over two successive years. Mycorrhizal inoculation significantly increased root colonization. G. mosseae-inoculated plants in both years exhibited a twofold higher root colonization than the indigenous mycorrhizal colonization. Compared with non-inoculated plants, mycorrhizal inoculation increased wheat yield for both years. In addition, increasing P fertilizer levels enhanced the wheat grain yield. In both years, the inoculum efficiency (IE) decreased with increasing P level addition. Phosphorus efficiency is higher under low P application than the higher P application. However, with mycorrhizal inoculation P efficiency is higher than the non-inoculated treatment. The effects of mycorrhizal inoculation on plant nutrient concentrations were determined: mycorrhiza-inoculated plants exhibited a higher zinc (Zn), manganese (Mn), copper (Cu), iron (Fe) nutrients concentration than noninoculated plants. After two years of field experiments, it is concluded that mycorrhizal inoculation can be used in large arable areas; however, it is also very important to manage the indigenous mycorrhiza of arable land.
Applied Soil Ecology, 2003
Volcanic ash-derived soils in Chile show very unique behavior and properties as soil system due to their unusual composition characterized by high allophane and stabilized humus content. These soils constitute excellent models to study both natural and man-induced VA mycorrhizal effect over plant nutrition and soil ecology sustainability. This paper studies the effect of Glomus etunicatum inoculation of wheat in a natural volcanic soil fertilized with soluble P or with partially acidulated-rock phosphate (pa-RP) at two rates (17 and 86 kg P ha −1); yield, plant phosphorus acquisition, and mycorrhizal colonized root length, are measured. The influence of these treatments on mycorrhizal mycelium and spore production as well as on soil phosphatase (P-ase) activity was also determined. The inoculation of G. etunicatum, locally isolated, increased significantly the extent of P plant acquisition, spore number, length of extraradical mycelium, and P-ase activity when compared with indigenous arbuscular mycorrhizal (AM) fungi fertilized with pa-RP, at the level of 86 kg ha −1. In concordance with these results, the remaining available P in the experimental soil without inoculation was depressed. A negative impact of soluble P application in G. etunicatum inoculated soil was noted in the P-ase activity, and also in the effectiveness of the applied inoculum in relation to P plant uptake. In soil AM inoculated and fertilized with pa-RP (86 kg ha −1), the enhancement of P-ase activity was related to high mycelium development and spore formation. P plant acquisition in G. etunicatum inoculated plants ranged from 4.96 to 11.57 mg per pot when 86 kg ha −1 of pa-RP is applied compared with the same amount of soluble P. Surprisingly, adding pa-RP does not improve the amount of colonized root length. Fungal root AM colonization and AM propagules (mycelium and spores) were not depressed at higher soluble P supply, but the activity of G. etunicatum measured as P plant uptake was strongly affected. The inoculation with G. etunicatum enhances spore production, particularly at the lower soluble P and at the highest pa-RP levels. A close relationship (r = 0.938) between AM spores and P-ase activity was found. Only in pa-RP treatments the G. etunicatum inoculation reduced the external P wheat requirements. The improvement of P-ase activity by G. etunicatum inoculation, as a biological factor involved in P-cycling in soil, may be an important mechanism related to plant P acquisition. It is concluded that pa-RP is the best source of P, not only by increasing P uptake by interacting with G. etunicatum, but also by enhancing AM propagules (mycelium and spores) remaining in soil.
Field Crops Research, 2012
The potential effect of indigenous and selected mycorrhizal fungal inoculation and phosphorus (P) treatment on plant growth, yield, root infection and inoculation effectiveness (IE) were tested with and without methyl bromide (MBr) for three successive years under field conditions. In 1997-1999, twelve plant species were used as host plants in a Menzilat soil series (Typic Xerofluvents) in the Mediterranean coastal region of Turkey. Compared to non-inoculated control plants, mycorrhizal inoculation increased yield in some years, but not in others. The mycorrhizal inoculum increased the root colonization of garlic, horsebean, soybean, chickpea, melon, watermelon, cucumber, maize, cotton, pepper, eggplant and tomato plants compared with the non-inoculated treatments. Compared to fumigation, plant roots grown in non-fumigated soil and successfully infected by indigenous mycorrhiza, resulted with better plant growth. Plant species belonging to the Solanaceae, Leguminosae, and Cucurbitaceae showed high responses to the mycorrhizal inoculation effectiveness under both fumigated and non-fumigated soil conditions. In general, IE was higher under low P supply than under high P supply. The effects of mycorrhizal inoculation on plant P and Zn concentrations were determined: mycorrhiza-inoculated plants had a higher nutrient content than non-inoculated plants, and this was most pronounced under fumigated soil conditions. After 3 years of field experiments, it has been concluded that for (seeded) field crops, soil and plant management systems make a great contribution to indigenous mycorrhiza to improve plant development. Whereas for horticultural plants, on the other hand, (plants transplanted into the field as seedlings), mycorrhizal inoculation makes it easy to use for large agricultural areas compared with the non-inoculated plants. It can be suggested to the farmers that arbuscular mycorrhizal fungus inoculated seedlings can be used under field conditions for high yield and quality.
Journal of Plant Nutrition, 2003
Greenhouse experiment was conducted to evaluate the effect of arbuscular mycorrhizal fungi (AMF) on plant growth, and nutrient uptake in saline soils with different salt and phosphorus (P) levels. The following treatments were included in this experiment: (i) Soil A, with salt level of 16.6 dS m−1 and P level of 8.4 mg kg−1; (ii) Soil B, with salt level of 6.2 dS m−1 and P level of 17.5 mg kg−1; and (iii) Soil C, with salt level of 2.4 dS m−1 and P level of 6.5 mg kg−1. Soils received no (control) or 25 mg P kg−1 soil as triple super phosphate and were either not inoculated (control) or inoculated with a mixture of AM (AM1) and/or with Glomus intraradices (AM2). All pots were amended with 125 mg N kg−1 soil as ammonium sulfate. Barley (Hordeum vulgar L., cv. “ACSAD 6”) was grown for five weeks. Plants grown on highly saline soils were severely affected where the dry weight was significantly lower than plants growing on moderately and low saline soils. The tiller number and the plant height were also lower under highly saline condition. The reduced plant growth under highly saline soils is mainly attributed to the negative effect of the high osmotic potential of the soil solution of the highly saline soils which tend to reduce the nutrient and water uptake as well as reduce the plant root growth. Both the application of P fertilizers and the soil inoculation with either inoculum mixture or G. intraradices increased the dry weight and the height of the plants but not the tiller number. The positive effect of P application on plant growth was similar to the effect of AM inoculation. Phosphorus concentration in the plants was higher in the mycorrhizal plant compared to the non mycorrhizal ones when P was not added. On the other hand, the addition of P increased the P concentration in the plants of the non mycorrhizal plants to as high as that of the mycorrhizal plants. Iron (Fe) and zinc (Zn) uptake increased with AM inoculation. The addition of P had a positive effect on micronutrient uptake in soil with low level of soil P, but had a negative effect in soil with high level of soil P. Micronutrient uptake decreases with increasing soil salinity level. Inoculation with AMF decreases sodium (Na) concentration in plants grown in soil of the highest salinity level but had no effect when plants were grown in soil with moderate or low salinity level. The potassium (K) concentration was not affected by any treatment while the K/Na ratio was increased by AM inoculation only when plant were grown in soil of the highest salinity level.
Journal of Plant Nutrition, 2019
The cultivation of horticultural crops, such as green peppers, tomatoes, eggplants and bell peppers is very common in semi-arid Mediterranean climate conditions. Two field experiments were performed to determine the effect of mycorrhizal species, plant species and phosphorus levels on mycorrhizal effectiveness and phosphorus (P) and zinc (Zn) nutrient uptake. In the first experiment, under field conditions, four plants species were inoculated with five arbuscular mycorrhizae (AM) species. In the second field experiment, under the same soil conditions, the same plant species were treated with three levels of phosphorus (P), i.e., control; 50 kg and 100 kg P 2 O 5 ha À1. The most effective mycorrhiza species Claroideoglomus etunicatum selected in the first experiment was used in the second field first experiment. In the first experiment, fruit yield enhancement, yield increase, inoculation effectiveness and nutrient concentration in the plant leaves were analyzed. Under field conditions, plant species growth is strongly dependent on the species of AM fungi. Tomato and green pepper plants were inoculated with Cl. etunicatum, eggplants were inoculated with Funneliformis mosseae and bell peppers were inoculated with Rhizophagus clarus, which are high fruit-yielding plant species. In general, Fu. mosseae and Cl. etunicatum increased the yield of the tomatoes, green peppers and eggplants. It seems mycorrhiza species specific to plant species. In the second experiment, mycorrhizal inoculation with P fertilizer application, in particular a moderate amount of P (50 kg ha À1 P 2 O 5) fertilizer increased the green pepper, bell pepper and tomato fruit yield compared with noninoculated plants and non-P fertilizer application treatments. Increasing the application of P level reduced the mycorrhizal inoculation effectiveness (MIE). The results indicate that for all four solanaceae family plants 50 kg ha À1 P 2 O 5 is a P level threshold for mycorrhizal development, which enhanced plant growth and addition of fertilizer over 50 kg ha À1 P 2 O 5 reduced MIE. P and Zn uptake were significantly increased with mycorrhizal inoculation. These findings are supported by our hypothesis that mycorrhiza inoculation can reduce mycorrhizal dependent horticultural plants P fertilizer requirement.
Plant development in a mycorrhizal field-grown mixture
Soil Biology and Biochemistry, 1991
In the field, a mycorrhiil mixture of corn and soybean was compared to non-mycorrhixal and to P-compensated plant mixtures. The extent of "N-transfer from soybean to corn was assess&. Plant development and the competitive relationship between the components of the mixtures were also examined. After having labelled selected soybean plants with isotopic NH,NO, by feeding roots induced on their stems, a greater amount of i5N-transfer to corn was measured in mycorrhixa inoculated plots than in control plots. The growth of both corn and soybean plants was greatly enhanced when inoculated with Glomur intraradix, and the effect of the fungus could not be replicated by fertilization. Inoculation and P fertilization had similar effects on P, K and Mg uptake by plants, but their effects differed regarding Ca absorption. Inoculation with the mycorrhixal fungus favoured the grass component of the mixture over the legume. Even if more N appeared to be transferred from soybean to corn when plants were mycorrhixal, the nutrient status of the plants suggests that the growth increase can be attributed mainly to a better P uptake by mycorrhizal plants, and that the significance of interspecific mycorrhixae-mediated N-transfer may be limited.