Phytochemical Screening, GC-MS Analysis and Biological Activities of Extracts of Artemisia vulgaris Linn (original) (raw)
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AMB Express
Artemisia species are important traditional medicinal plants of Ethiopia which are used for the treatment of infection and non-infection health problems. The genus Artemisia (Astraceae) consists of about 500 species worldwide. In this study, the main aim was to investigate chemical components of Artemisia spp. (A. abyssinica, A. absinthium and A. annua) and evaluate their antimicrobial activities against bacterial strains. The results indicated that the crude extract of these plants was effective against selected bacterial strains. Here we isolated the well-known antimalarial drug artemisinin (7 mg, 0.004%) from Artemisia annua leaves using a rapid n-hexane fractionation method. The n-hexane extract of A. abyssinica, ethyl acetate extract of A. absinthium and n-hexane of A. annua showed varying degrees of inhibiting effect against bacterial strains such as Staphylococcus aureus ATCC 25923 T, Salmonella enteritidis ATCC13076T, Klebsiella pneumoniae ATCC1053T, boydii ATCC1233T, Escher...
Antimicrobial Activity of Leaves of Artemisia Vulgaris L
Proceedings of The 16th International Electronic Conference on Synthetic Organic Chemistry
Objective: To screen the antimicrobial activity of different extracts of leaves of Artemisia vulgaris. Materials and Methods: To detect the in vitro antibacterial activity, 10 bacterial strains were selected. These bacteria are both gram +ve and gram-ve. Leaves were extracted with a petroleum ether, chloroform, ethyl acetate, ethanol and aqueous. In the present work the antibacterial activity was done by cup plate method. The antibacterial activity was expressed as zone diameter in millimeters. Different extracts from leaves of the plant was compared with standards like benzyl penicillin for gram +ve bacteria and streptomycin for gram-ve bacteria using DMF as control. The readymade media for inoculum and culture was obtained from Himedia labs. For antifungal activity four fungal organisms were selected and Griseofulvin was used as standard. Results: Herbal extracts prepared from the leaves of the plant were screened against bacteria and fungal organisms at the concentration range between 50 µg and 300 µg/0.1ml. The results of antimicrobial activity revealed that the extract exhibited activity against both gram +ve, gram-ve and fungal organisms. Conclusion: The present investigation reveals that the aqueous, chloroform and ethyl acetate extracts and in some cases petroleum ether extract showed significant antimicrobial activity when compared with standard.
Nutritional, Phytochemical, Antioxidant and Antimicrobial Potential of Artemisia Herba-Alba (ASSO.)
2020
This study estimated the various components of the aerial parts of Artemisia herba-alba belonging to family Asteraceae. The components extracted from the plant were elucidated by GC/MS spectroscopic analysis. The data revealed that the extract contains 2-methyl-pentane (0.09%), borneol (0.10%), Camphor (97.61%), caryophyllene oxide (0.10%), t-cadinol (0.09%), preg-4-en-3-one, 12,17-dihydroxy-17-cyano(0.12%), androstane-3,17-dione (1.74%), cholest-22-ene-21-ol, 3,5-dehydro-6methoxy pivalate (0.02%) and geranyl isovalerate (0.01%). The nutritional value, phytochemicals, antioxidant and antimicrobial activity were studied using several assays following standard procedures. The nutritional results indicated that aerial parts of A. herba-alba are furious source of dietary fiber, carbohydrates, proteins and lipids. The results indicated that the prepared water and methanol extracts expressed high antioxidant scavenging activity. The extracts expressed antimicrobial activity against severa...
Molecules
Artemisia vulgaris is an enormously useful aromatic plant known for its insecticidal, antifungal, parasiticidal, and medicinal values. The main aim of this study is to investigate phytochemical contents and the potential antimicrobial activities of Artemisia vulgaris essential oil (AVEO) from the fresh leaves of A. vulgaris grown in Manipur. The AVEO isolated by hydro-distillation from A. vulgaris were analyzed by gas chromatography/mass spectrometry and solid-phase microextraction-GC/MS to describe their volatile chemical profile. There were 47 components identified in the AVEO by GC/MS, amounting to 97.66% of the total composition, while 97.35% were identified by SPME-GC/MS. The prominent compounds present in AVEO analyzed by direct injection and SPME methods are found to be eucalyptol (29.91% and 43.70%), sabinene (8.44% and 8.86%), endo-Borneol (8.24% and 4.76%), 2,7-Dimethyl-2,6-octadien-4-ol (6.76% and 4.24%), and 10-epi-γ-Eudesmol (6.50% and 3.09%). The consolidated component...
Antimicrobial effects and Phytoconstituents of ethanolic extract of leaves of Artemisia annua L.
Tel: +2348062685063] Studies were carried out on antimicrobial effects and phytoconstituents of Ethanolic extract of leaves of Artemisia annua L. The extract significantly inhibited the test organisms. The minimum inhibitory concentration value of the ethanolic extract was 125 mg/ml for each of the test organisms: Escherichia coli, Streptococcus faecalis, Pseudomonas auroginosa, Klebsiella pneumoniae, Candida albicans and Staphylococcus aureus. The result of the minimum bactericidal concentration showed that, the extract was bactericidal at the concentration of 250 mg/ml. The phytochemical analysis revealed the presence of Tannins, Flavonoids, Cardiac Glycosides, Alkaloids, Phenols and Resins.
Medicinal plants are potential sources of natural compounds with biological activities and therefore attract the attention of researchers worldwide. The objective of this research was to determine the chemical composition of methanolic flowers extract. The phytochemical compound screened by GC-MS method. Forty nine bioactive phytochemical compounds were identified in the methanolic extract of Artemisia annua. The identification of phytochemical compounds is based on the peak area, retention time molecular weight, molecular formula, MS Fragment-ions and pharmacological actions. GC-MS analysis of Artemisia annua revealed the existence of the 1,2-15,16-Diepoxyhexadecane, 1-Methylcycloheptanol, 3,5-Hexadien-2-ol2-methyl, Cholestan-3-ol ,2-methylene, (3ß,5α), 2,5-Octadecadiynoic acid , methyl ester, Cyclohexene,1-methyl-5-(1-methylethenyl), Cyclohexene,4-isopropenyl-1-methoxymethoxymethyl, Exo-2,7,7-trimethylbicyclo[2.2.1]heptan-2-ol, 2(3H)-Furanone,5-ethenyldihydro-5-methyl, 2H-Benzo[f]...
ANALYSIS OF ARTEMISININ ISOLATED FROM Artemisia Annua L. BY TLC AND HPLC
Journal of Liquid Chromatography & Related Technologies
Artemisia annua L. (Quinghao) is a promising and potent source of antimalarial herbal drug. Its activity has been ascribed to the content of artemisinin; it has been analyzed by different chromatographic techniques. In this research, we have developed a TLC/UV, a simply available, rapid and cost efficient method, for the simultaneous determination of artemisinin. The crude extracts from plant samples were used together with a standard in our HPLC/UV and TLC/UV techniques. Artemisinin (standard and in the extract) was converted into a UV-absorbing compound, Q258 by being treated with 0.25% (by weight) NaOH solution and then 0.2 M acetic acid. The resulting TLC method utilizes separation on silica gel RP-18 with methanol, acetonitrile, ethyl acetate, acetic acid (30:20:2:1) as mobile phase, highly specific densitometric evaluation at 254 nm. This precise and accurate assay could be performed in the linear working range of 10-100 µg/ml and 1-40 µg/ml for TLC and HPLC methods respective...
Molecules, 2021
Diverse medicinal plants such as those from the genus Artemisia have been employed globally for centuries by individuals belonging to different cultures. Universally, Artemisia species have been used to remedy various maladies that range from simple fevers to malaria. A survey conducted by the World Health Organization (WHO) demonstrated that 80% of the global population is highly reliant on herbal medicine for their primary healthcare. WHO recommends artemisinin-based combination therapies (ACT) for the treatment of global diseases such as malaria. Artemisinin is a bioactive compound derived from Artemisia annua leaves. It is a sesquiterpene endoperoxide with potent antimalarial properties. This review strives to instill natural products to chemists and others in diverse fields with a heterogeneous set of knowledge compiled from multifaceted researchers and organizations in literature. In particular, the various Artemisia species and effective extraction, isolation, and characteriz...
Comparison of Artemisia annua Bioactivities between Traditional Medicine and Chemical Extracts
Current Bioactive Compounds, 2014
The present work investigates the efficacy of using Artemisia annua in traditional medicine in comparison with chemical extracts of its bioactive molecules. In addition, the effects of location (Egypt and Jericho) on the bioactivities of the plant were investigated. The results showed that water extracts of Artemisia annua from Jericho have stronger antibacterial activities than organic solvent extracts. In contrast, water and organic solvent extracts of the Artemisia annua from Egypt do not have anti-bacterial activity. Furthermore, while the methanol extract of EA displayed high anticancer affects, the water extract of Egypt and the extracts of Jericho did not show significant anticancer activity. Finally, the results showed that the methanol and water extracts of Jericho had the highest antioxidant activity, while the extracts of Egypt had none. The current results validate the scientific bases for the use of Artemisia annua in traditional medicine. In addition, our results suggest that the collection location of the Artemisia annua has an effect on its chemical composition and bioactivities.
International Journal of Science and Research (IJSR)
The oil obtained by hydro distillation of Artemisia vulgaris using Clevenger apparatus revealed presence of alkanes, oxygenated and non-oxygenated monoterpenes, and sesquiterpenes. The oxygenated monoterpenes were either alcohols or esters, and the oxygenated sesquiterpenes were in the form of acetates and oxides. AVO1 contained 70 different constituents whereas AVO2 contained about 42 different chemical constituents. The physiochemical characters showed that the yield percentage varied from 1.43 to 1.28, acid value varied from 0.397 to 0.45and specific gravity varied from 0.878 to 0.902 in AVO1 and AVO2 respectively. Likewise the oil was found to be insoluble in water and fixed oil and soluble in most organic compounds. Volatile oil from both the altitude showed a significant antibacterial and antifungal effect. A large inhibition was seen in S. aureus, E.coli and enterococci species. Both the sample showed antifungal activity against Candida species.