Detection of Cytokeratin 19 Mrna in Blood as an Early Marker of Micrometastatic Tumor Cells of Nasopharyngeal Carcinoma Patients in Surabaya (original) (raw)
Nasopharyngeal carcinoma (NPC) is a unique epithelial malignancy triggered by Epstein Barr virus that occurs at a high frequency in certain regions of southeast Asia. Previous studies reported that this tumor was a radiosensitive but the predominant failure after adequate radiotherapy was due to its distant metastasis. It would be of value to detect the presence of metastasis as early as possible. Previous studies have shown that cytokeratin 19 (Ck 19) positive cells indicate the presence of micrometastasis. The purpose of this study was to evaluate the presence of Ck 19 mRNA by nested RT-PCR in established NPC patients and its correlation to the various stages of NPC as determined by conventional clinical examination. The reported sensitivity of RT-PCR was around 1/107, i.e one cancer cell can be detected among 107 normal blood cells. Venous blood samples from 30 patients with biopsy-proven nasopharyngeal carcinoma and 10 ENT non NPC patients were tested. After we isolated total mR...
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