Molecular characterization of Antimicrobial-Resistant Escherichia coli isolated from broiler chickens (original) (raw)
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Bacteriological and Molecular Studies on E. Coli Isolated from Broiler Chickens
Assiut Veterinary Medical Journal, 2020
Avian colibacillosis is one of the most important diseases of chickens, resulting in high economic losses as well as high morbidity and mortality. In the current study, the prevalence of avian colibacillosis was studied in 200 broiler chickens obtained from different farms in Beni-Suef and El-Fayoum Governorates. A total of 200 pooling samples were collected aseptically from heart blood as well as the affected internal organs including airsacs, pericardial sac, liver, lung and ascetic fluids of slaughtered diseased and freshly dead broiler chickens. Bacteriological examination of the collected samples showed that a total of 58 E. coli isolates were recovered with a prevalence of 29%. The results of in-vitro antimicrobial susceptibility tests revealed that E. coli isolates showed high sensitivity to colistin sulphate only (72.4%). On the other hand, high resistances were recorded to all other antimicrobials including cefotaxime sodium and florophenicol (96.6% for each), apramycin, ci...
Alexandria Journal of Veterinary Sciences, 2019
Avian pathogenic Escherichia coli (APEC) represent a pathotype of enteric bacteria that affects avian species causing colibacillosis, a severe and most common infectious disease of farmed poultry. Colibacillosis is one of the serious problems affecting poultry industry because it is responsible for a variety of disease conditions such as colisepticemia, air sacculitis, peritonitis, perihepatitis, pericarditis, omphalitis, coligranuloma, enteritis, synovitis, swollen head syndrome and osteomyelitis (
Egyptian Journal of Veterinary Sciences
T HIS study aimed to evaluate the most predominant strain of avian pathogenic Escherichia coli (APEC) that causes colibacillosis with respiratory manifestation in broiler chickens. Isolation and serotyping identification for the isolates of E. coli were performed. Prevalence of APEC differentiated by detection of the 8 virulence-associated genes "astA, iss, irp2, papC iucD, tsh, vat, and cva/cvi" using multiplex PCR technique in the most important isolates of E. coli was also investigated. Antibacterial sensitivity test for antibiotics disc and oregano at different concentrations (20%, 15%, 10%, and 5%), were estimated for the most important isolates of E. coli. A total of 50 broiler farms were located in Gharbia, Giza, Menoufia, Bahaira, and Dakahliah Governorates were examined. Specimens from lung, liver, spleen, and kidney from diseased birds were used for bacteriological examination. Results revealed that E. coli O27 was the most predominant serotype strain (11 out of 35 isolates), followed by the serotypes of O146 (4/35), O Untypable serotypes (4/35), and the (1/35) of each serotype of O8,
Assiut Veterinary Medical Journal, 2022
The main serotypes, pathogenicity, and antibiotic susceptibility of Avian Escherichia coli isolated from Colibacillosis cases in the Aljabel Al Akhdar region of Libya were investigated in this study. To determine the pathogenicity of E.coli isolates, Congo red binding, hemolysis, and lethality tests were conducted on Seventy-two unvaccinated broilers chicks on the day of hatching. Avian pathogen serotypes and antibacterial susceptibility testing to twelve antibiotics were also performed according to the standard methods. Results revealed that the only (O114) serotype that had been orally inoculated caused mortality on the first day. While all E.coli isolates inoculated by subcutaneous route showed mortality on the first day. The highly pathogenic serotypes were (O26) induced (100%) mortality. Congo red (CR) binding assay showed that (100%) of isolates were positive. Only (O26) was found to be hemolytic. Serotyping results revealed that E.Coli strains belong to serotypes (O26, O111 O...
Phenotypic characterization of Escherichia coli isolated from broiler chickens
Ismail A. Radwan 1, Sabry M. Tamam 2, Abeer A. El Sayed 3, Rania O. Qurani 1 , 2019
Colibacillosis is one of the most important diseases of chickens, resulting in significant losses. The current study aimed to investigate the prevalence of E. coli infections in broiler chickens detecting their phenotypic characters such as Congo red binding activity, serum resistance and proteolytic activities. Samples were collected from 297 broiler chickens of different ages from different farms in El-Fayoum Governorate during the period from April 2017 up to March 2018. Bacteriological examination showed that 98 E. coli isolates were recovered with a prevalence rate of 33%. Results of Congo red binding activity and serum resistance revealed that all E. coli isolates (100%) showed Congo red binding activity and survive for 1 and 6 hrs and grown for 18 hrs in the presence of serum. Results of proteolytic activity revealed that 43 E. coli isolates (43.9%) were able to digest casein of skimmed milk while 16 isolates (16.3%) were positive for gelatin liquefaction test.
Acta Scientifci Nutritional Health
Poultry has been on earth for over 150 million years, dating back to the original wild jungle fowl. Ducks, geese, turkeys, pigeons, and chickens are on the list of the species under the general term poultry [1]. In Sudan, poultry products were commenced on a commercial basis in 1979 by Sudanese Kuwaiti poultry production Company. As a commodity, poultry products are highly demanded by the locals and the public of the neighboring countries especially Arabs [2]. Poultry meat is an important food product and constitutes a substantial portion of daily-consumed proteins. It is the second most widely eaten type of meat in the world, i.e. chicken, turkey, duck, geese, domesticated quail [3].
African Journal of Microbiology Research, 2013
In Setif East Algeria, one hundred and eighty Escherichia coli strains were isolated frombroiler chickens with colibacillosis and were examined for susceptibility to antimicrobials of veterinary significance. In vitro antibiotic activities of 12 antibiotic substances against the isolates were determined by disc diffusion test. Antibiogram revealed a high level of resistance to enrofloxacin (72.2%), neomycin (75%), trimethoprim-sulfmethoxazole (82.2%), ampicillin (84.5%) and amoxicillin (87.8%), nalidixic acid (96.7%) and doxycycline (98.3%). There were moderate levels of resistance to chloramphenicol (45.6%) and streptomycin (66.1%). There were low levels of resistance to nitrofurantoin (18.9%), colistin (5.5) and gentamicin (5.5%). All strains were multi-drug resistant and more than half (56.1%) of the isolates were resistant to eight antibiotics. Thus, 60 antibiotic resistant patterns in E. coli strains were isolated, of which 13 were present significantly. These findings suggest ...
Veterinary World, 2024
Background and Aim: Colibacillosis is caused by avian pathogenic Escherichia coli (APEC), which results in significant losses for the poultry sector. It has zoonotic potential and acts as a source of antibiotic resistance and virulence genes for other E. coli. This study aimed to assess phylogenetic groups, virulence factors, and resistance phenotypes of APEC strains isolated from broiler farms in Northern Palestine. Materials and Methods: A total of 65 APEC isolates were recovered from diseased chickens with typical colibacillosis symptoms from broiler farms located in the northern region of Palestine from May to July 2024. Strains were identified using classical and molecular techniques. Antibiotic resistance was detected using the disk diffusion method. Phylotyping and virulence genotyping of the APEC isolates were performed using a polymerase chain reaction (PCR). Results: This study showed a high detection rate of APEC strains (100%) in chickens. The most APEC strains, 56/65 (86.2%), belonged to group D. Other strains were assigned to groups B2 (5/65, 7.7%), B1 (3/65, 4.6%), and A (1/65, 1.5%). Antibiotic resistance ranged from 27.7% for Polymyxin E (colistin) to 100% for Amoxicillin. Polymyxin E (colistin) and fosfomycin are the most effective drugs. The most common virulence gene was iroN, which was detected in 61 isolates (93.8%). The APEC strains in Palestine exhibit a wide variety of resistance patterns and genetic variations. Conclusion: Controlling APEC infections is essential for public health, especially when APEC isolates can pass on resistance and virulence genes to dangerous bacteria such as E. coli that are particular to humans. It is essential to understand APEC pathogenesis, antimicrobial therapy, and the development of measures to control colibacillosis. Keywords: antibiotic resistance, avian pathogenic Escherichia coli, colibacillosis, Palestinian territories, phylogenetic group, virulence factors.
African Journal of Microbiology Research, 2015
In East Algeria, 100 Escherichia coli strains were isolated from livers and spleens of 120 broiler carcasses that presented colibacillosis lesions at autopsy. Serogrouping of these strains demonstrated that 83% of their serogroups belong to the most pathogenic serogroups of Avian Pathogenic Escherichia coli (APEC): O1 (14%), O2 (53%) and O78 (16%). In vitro susceptibility to antimicrobials of veterinary significance was determined by disc diffusion test. Antibiogram demonstrated a high level of resistance to enrofloxacin (82%), trimethoprim-sulfmethoxazole (82%), ampicillin (89%) and amoxicillin/ Ac clavulanic (90%), nalidixic acid (99%) and tetracycline (100%). There were moderate levels of resistance to neomycin (49%) and nitrofurantoin (53%). There were low levels of resistance to gentamicin (2%), chloramphenicol (13%) and no resistance to colistin sulfate (0%). All strains were multi-drug resistant and more than half (58%) of the isolates were resistant to seven antibiotics at l...
Molecular Analysis of Escherichia coli Strains Causing Respiratory Troubles in Chickens in Egypt
Zagazig Veterinary Journal, 2019
Colibacillosis is a complicated disease causing severe economic losses and challenging veterinarians and producers. Therefore, this study aimed to characterize avian pathogenic Escherichia coli (APEC) strains causing respiratory signs in chickens. Thirty broiler chicken flocks at age of 17-35 days showed respiratory signs and greenish diarrhea during 2013-2016 outbreaks that occurred in Sharkia, Ismailia, Dakahlia and Sinai Governorates. The postmortem findings revealed typical colisepticemia picture including air sacculitis, fibrinous pericarditis and perihepatitis. The percentage of APEC isolation was 100%. Mixed bacterial infections with Enterobacter aerogenes or Providencia rettgeri (3 flocks, each), Klebsiella pneumoniae (2 flocks), Serratia liquefaciens or Enterobacter agglomerans (1 flock, each) was evidenced. From 284 collected samples (air sacs, heart blood, lungs and liver), E. coli was predominantly isolated from air sacs (76.1 %) and lung (73.2 %) followed by heart blood (67.6%) and liver (54.9%). Based on serogrouping, the most common serogroups were O78 and O2 with percentages of 15%, each. Utilizing antimicrobial disc diffusion test, the isolates showed 32.7% resistance to doxycycline and 100% resistance to lincomycin, spiramycin, oxacillin and amoxicillin. Polymerase chain reaction (PCR) analysis for 55 MDR E. coli isolates from air sac and heart blood revealed 3 β-lactamase resistance genes; blaTEM (87.3 %), blaCTX-M (85.5 %) and blaOXA (5.5 %) and 6 virulence genes in two multiplex PCR; iucD (96.4%), Fim H (92.7%), iss (76.4%), ompT (58.2%), tsh (45.5%) and cvaC (9.1%). An association of virulence with multidrug resistance genes in E. coli was recorded, that hindered the control measures. Therefore, alternative strategies were necessary to minimize the antibiotic use and reduce the virulent strains' occurrence.