Antibiotic Susceptibility Pattern, Molecular Characterization of Virulence Genes among Pseudomonas aeruginosa Isolated from Burn Patients (original) (raw)

In this research, a total of 150 samples were obtained from burn and wound patients admitted to the West Erbil Emergence Hospital during period from September 2020 to January 2021. Through cultural, morphological features, biochemical testing, and Vitek 2 Compact Systems, 40 isolates of P. aeruginosa have been identified. P. aeruginosa produced various pigments, including blue/green and yellow/green. The isolates of P. aeruginosa were subjected to 14 different antibiotics. Imipenem was the most effective antimicrobial agents against all P. aeruginosa isolates, and most of isolates showed high resistance degree to ampicillin 100%, chloramphenicol 100%, amoxicillin-clavulanic acid 100%, cefotaxime 100%, and penicillin 100% while for aztreonam 32.5%, meropenem 42.5%, tobramycin 45%, gentamycin 45%, amikacin 45%, ciprofloxacin 62.5%, ceftazidime 67.5%, and tetracycline 80%. All Pseudomonas aeruginosa isolates were screened using multiplex polymerase chain reaction (PCR) to check for the presence of Pvda, LasB, Protease, exoA, exoT, exoU, and plch on its genomic DNA. The findings have shown that Pvda was 55%, LasB 75%, protease 65%, exoA 60%, exoT 75%, exoU 60%, and plch 55% of isolates harbored these genes as a virulence genes.