Extended Spectrum Beta-Lactamase Escherichia coli in River Waters Collected from Two Cities in Ghana, 2018–2020 (original) (raw)
Related papers
2015
Untreated wastewater is a risk factor for the spread of antibiotic resistance in the environment. However, little is known about the contribution of untreated wastewater to the burden of antibiotic resistance in the Nigerian environment. In this study, a total of 143 ceftazidime-/cefpodoxime-resistant bacteria isolated from untreated wastewater and untreated wastewater-contaminated surface and groundwater in Nigeria were screened for extended-spectrum β-lactamase (ESBL) genes, integrons and integron gene cassettes by PCR. The genetic environment of bla CTX-M-15 was mapped by PCR and potentially conjugative plasmids were detected among the isolates by degenerate primer MOB typing (DPMT). ESBL production was confirmed in 114 (79.7%) isolates and ESBL genes (bla SHV , bla CTX-M-15 and bla TEM) were detected in 85 (74.6%) ESBL-producing isolates. bla CTX-M-15 was associated with ISEcp1 and with orf477 in 12 isolates and with ISEcp1, IS26 and orf477 in six others. To the best of our knowledge, this is the first report of bla CTX-M-15 in hand-dug wells and borehole serving as sources of drinking water and a first report of the genetic environment of bla CTX-M-15 in environmental bacteria from Nigeria. The results of this study confirm untreated wastewater as an important medium for the spread of ESBL-producing bacteria within the Nigerian environment. Hence, the widespread practice of discharging untreated wastewater into the aquatic ecosystem in Nigeria is a serious risk to public health. Keywords Antibiotic resistance. β-Lactamases. bla SHV. bla TEM. bla CTX-M-15. Conjugative relaxase. Untreated wastewater
PLOS global public health, 2023
Background Extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-Ec) represents a significant global public health concern. The epidemiology of ESBL-Ec in Uganda is not well understood although it is harbored by humans, animals, and the environment. This study explains the epidemiology of ESBL-Ec using a one health approach in selected farming households in Wakiso district, Uganda. Methodology Environmental, human, and animal samples were collected from 104 households. Additional data were obtained using observation checklists and through interviews with household members using a semi-structured questionnaire. Surface swabs, soil, water, human and animal fecal samples were introduced onto ESBL chromogenic agar. The isolates were identified using biochemical tests and double-disk synergy tests. To assess associations, prevalence ratios (PRs) were computed using a generalized linear model (GLM) analysis with modified Poisson and a log link with robust standard errors in R software.
BackgroundExtended-spectrum beta-lactamase-producingEscherichia coli(ESBL-PE) represents a significant global public health concern. Much as humans, animals and environments harbor ESBL-PE, its epidemiology in Uganda is still not well understood. This study explains the epidemiology of ESBL-PE using the one health approach in selected farming households in Wakiso district, central Uganda.MethodologyEnvironmental, human, and animal samples were collected from 104 households. Additional data were obtained using observation checklists and through interviews with household members using a semi-structured questionnaire. Surface swabs, soil, water, human and animal fecal samples were introduced onto ESBL chromogenic agar. The isolates were identified using biochemical tests and double-disk synergy tests. To assess associations, prevalence ratios (PRs) were computed using a generalized linear model (GLM) analysis with modified Poisson and a log link with robust standard errors in R softwar...
Antibiotics
Wastewater treatment plants (WWTPs) are major reservoirs of antibiotic-resistant bacteria (ARB), favouring antibiotic resistance genes (ARGs) interchange among bacteria and they can provide valuable information on ARB circulating in a community. This study characterised extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli from the influent and effluent of four WWTPs in uMgungundlovu District, KwaZulu-Natal, South Africa. E. coli was enumerated using the membrane filtration method and confirmed using the API 20E test and real-time polymerase chain reaction. ESBL-producers were phenotypically identified by their susceptibility to the third-generation cephalosporins using the disc diffusion and the double-disc synergy methods against cefotaxime (30 µg) with and without 10 µg clavulanic acid. Genotypic verification was by PCR of the TEM, SHV, and CTX-M genes. The clonality of isolates was assessed by ERIC-PCR. The highest E. coli count ranged between 1.1 × 105 (influent) a...
PLOS ONE, 2019
Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli have been reported in natural environments, and may be released through wastewater. In this study, the genetic relationship between ESBL-producing E. coli collected from patient urine samples (n = 45, both hospitalized patients and outpatients) and from environmental water (n = 82, from five locations), during the same time period, was investigated. Three independent water samples were collected from the municipal wastewater treatment plant, both incoming water and treated effluent water; the receiving river and lake; and a bird sanctuary near the lake, on two different occasions. The water was filtered and cultured on selective chromID ESBL agar plates in order to detect and isolate ESBL-producing E. coli. Illumina whole genome sequencing was performed on all bacterial isolates (n = 127). Phylogenetic group B2 was more common among the clinical isolates than the environmental isolates (44.4% vs. 17.1%, p < 0.01) due to a significantly higher prevalence of sequence type (ST) 131 (33.3% vs. 13.4%, p < 0.01). ST131 was, however, one of the most prevalent STs among the environmental isolates. There was no significant difference in diversity between the clinical isolates (DI 0.872 (0.790-0.953)) and the environmental isolates (DI 0.947 (0.920-0.969)). The distribution of ESBL genes was similar: bla CTX-M-15 dominated, followed by bla CTX-M-14 and bla CTX-M-27 in both the clinical (60.0%, 8.9%, and 6.7%) and the environmental isolates (62.2%, 12.2%, and 8.5%). Core genome multi-locus sequence typing showed that five environmental isolates, from incoming wastewater, treated wastewater, Svartån river and Hjä lmaren lake, were indistinguishable or closely related (�10 allele differences) to clinical isolates. Isolates of ST131, serotype O25:H4 and fimtype H30, from the environment were as closely related to the clinical isolates as the isolates from different patients were. This study confirms that ESBL-producing E. coli are common in the aquatic environment even in low-endemic regions and suggests that wastewater discharge is an important route for the release of ESBL-producing E. coli into the aquatic environment.
Advances in Microbiology, 2015
Antimicrobial use in agriculture, livestock and human health has increased over the years leading to the increase in antimicrobial resistance that can also find its way to the aquatic environment. Rivers can act as reservoirs of highly resistant strains and facilitate the dissemination of multidrug resistant (MDR) strains to animals and humans using water. A total of 318 water samples were collected from six different sampling points along Athi River and E. coli isolates were subjected to Kirby-Bauer diffusion method for antimicrobial susceptibility testing. The total mean coliform count of the sampled sites was 2.7 × 10 4 (cfu/mL). E. coli isolates were most resistant to ampicillin (63.8%) and most susceptible to gentamicin (99.4%). MDR strains (resistance to ≥3 classes of antibiotics) accounted for 65.4% of all the isolates. The site recorded to have human industrial and agricultural zone activities had strains that were significantly more resistant to ampicillin, cefoxitin, amoxicillin/clavulanic acid (P ≤ 0.05) than isolates from the section of the river traversing virgin land and land with minimum human activities. This study indicates that E. coli strains isolated from Athi River were highly MDR and most resistant to some antimicrobial classes (ampicillin and cefoxitin) which constitute a potential risk to human and animal health.
2020
Introduction: Community-acquired infections due to extended-spectrum beta-lactamase (ESBL) producing Escherichia coli are rising worldwide, resulting in increased morbidity, mortality, and healthcare costs, especially where poor sanitation and inadequate hygienic practices are very common. Objective: This study was conducted to investigate the prevalence and characterization of multidrug-resistant (MDR) and ESBL-producing E. coli in drinking water samples collected from Rohingya camps, Bangladesh. Methods: A total of 384 E. coli isolates were analyzed in this study, of which 203 were from household or point-of-use (POU) water samples, and 181 were from source water samples. The isolates were tested for virulence genes, ESBL-producing genes, antimicrobial susceptibility by VITEK 2 assay, plasmid profiling, and conjugal transfer of AMR genes. Results: Of the 384 E. coli isolates tested, 17% (66/384) were found to be ESBL producers. The abundance of ESBL-producers in source water conta...
International Journal of Research Studies in Microbiology and Biotechnology, 2019
Increasingly, public water drinking sources in urban settlements could potentially harbor dangerous strain of Escherichia coli from numerous sources, such as from animal and human carriers. It is strongly believed that after drinking contaminated water, within few hours or days, the organisms will settle in the gut [1].Escherichia coli as the predominant facultative organism in the human intestinal tract hence, consist of a small proportion of the intestinal bacteria load burden. Nevertheless, the presence or detection of Escherichia coli in public water is an indicator of potential faecal contamination of myriad of sources [2].Escherichia coli has a fimbriae that is specie specific and each fimbriae adhesion help to attach them to specific receptor site on the enterocytes of the proximal small intestine [3].There are five classes of Escherichia coli that produce disease, based on their pathogenesis. They are Enterotoxigenic Escherichia coli, Enterpathogenic Escherichia coli, Enteropathogenic Escherichia coli. Enteroaggressive Escherichia coli and Enterohemorrhagic Escherichia coli [4]. Interestingly, the pathogens enter a water supply chain sporadically and do not survive for long periods of time; however, presence of Escherichia coli in water supplies also suggests that other intestinal pathogens could also be present, since they are all present in faeces found in the small Abstract: Escherichia coli contamination of drinking water source has continued to emerge, as an important public health concern across the globe. This had been associated with water born epidemic outbreak, especially in rural communities were access to potable water remains a massive challenge. However, identifying the source of Escherichia coli pollution in public water source had been hugely attributed to faecal contamination either from man or animals which constitute a serious environmental and public health threat to man. This study was designed and thus, aimed to determine the bacteriological quality of borehole water sources with specific reference to E. coli contamination in the studied locations (in Mile 2 and 3 area of Diobu, Port Harcourt).However, A convenience random sampling research design was explored to collect 50 water samples aseptically, and it was analyzed, using standard microbiological cultural technique (All samples were cultured on MacConkey and Nutrient agar plates respectively). The positive samples were examined for faecal coli form. Identification of Escherichia coli was done based on microscopic, cultural and biochemical characteristics. Of the 50 samples collected and processed for coli form presence, 22 samples (44%) showed growth of Escherichia coli when cultured on nutrient agar, 15 samples (30%) showed growth of Escherichia coli when cultural on MacConkey agar while 13 (26%) showed no growth on both media. This result strongly suggest that 74% of the boreholes studied did not meet up with the World Health Organization Standards guideline for drinking water source, thus will certainly pose a public health risk to the inhabitant of the area if left untreated. Nonetheless, , it is firmly recommendation that borehole water from these affected areas should be treated, boiled and filtered before use for drinking and washing of fresh fruits to avert the possibilities of an outbreak of the water borne epidemic in the area.
Journal of Applied Sciences and Environmental Management, 2017
Escherichia coli are known pathogenic organism that has caused diseases which has led to severe morbidity and increased death rate. The occurrence of extended spectrum beta Lactamase (bla) producing Escherichia coli has been on the rise. Water samples were investigated as a potential reservoir for the Extended Spectrum Beta-Lactamase (ESBL)-producing E. coli using phenotypic (culture-based) and molecular methods. Double disc synergy test was determined between a disc of amoxicillin-clavulanate (20µg/10µg) (augmentin) and a 30-µg disc of each thirdgeneration cephalosporin antibiotic placed at a distance of 20 mm from centre to centre on a Mueller-Hinton Agar plate streaked with the isolate. An isolate was considered to be ESBL negative if there was no enhancement between any of the cephalosporin and the clavulanate-containing discs and were then subjected to specific Polymerase Chain Reaction (PCR). Eighty-four environmental E. coli was isolated. 58(69.04%) showed positivity for ESBL production. E. coli isolates positive for ESBL-production selected and subjected to plasmid curing were all plasmid mediated. 16 isolates subjected to PCR to identify the presence of blaSHV (Sulphydryl Variable), blaTEM (Temoneira) and blaCTX-M (Cefotaximase) genes revealed that 11(68.7%) of these had at least one ESBL gene (either blaCTX-M or blaTEM, or both), 5(31.3%) isolates do not have any of the three ESBL genes, and blaSHV was not detected in any of the isolates. The results of this study indicate the widespread prevalence of ESBLs in E. coli. Therefore, beta-lactam antibiotics and beta-lactamase inhibitors should be prescribed based on an antibacterial susceptibility test. © JASEM