Comparison of the Effect of Adipose Mesenchymal Stem Cells-derived Secretome with and without Reovirus in CT26 Cells (original) (raw)
Colorectal cancer is the fourth leading cause-related death of cancer which has significantly increased over the past three decades. New therapeutic approaches such as oncolytic viruses become very imperative recently to destruction of cancer cells. Use of mesenchymal stem cells secretome which are produced in response to variant conditions involves different paracrine molecules secretion that they have therapeutic potential in several chronic diseases. Mesenchymal stem cells and their derivative are used as a regenerative medicine, but there is ambiguity in the function of these cells in control of malignancy. In this study, we aim to examine the apoptotic effect of secretome derived from mesenchymal stem cells affected by encompassing oncolytic reoviruses. Mesenchymal stem cells were cultured after separation from abdominal adipose tissue of BALB/c mice. After three passages, the cells infected by reovirus at Multiplicity of Infection (MOI) of 1 Plaque-Forming Unit (PFU) per cell....