Determination of Enzyme Inhibition and Antioxidant Activity in Some Chestnut Honeys (original) (raw)

Comparison of Antioxidant Enzymes in Natural and Commercial Honey

Pak-Euro Journal of Medical and Life Sciences

Honey is popular all over the world and used commonly in medicines and food. This study was designed to compare the natural and commercial honey for their antioxidant enzymes. In this study, 11 different honey samples were collected from different areas of Balochistan. The activity of different antioxidants like superoxide dismutase, peroxidase and catalase enzymes were measured and compared by standard curves of pure enzymes moreover, protein content was also determined by using Bradford Assay. Results showed that highest protein content was found in one of the natural honey samples (4.42 ± 0.02 U/mg) enzymes like superoxide dismutase showed highest activity of (4.93 ± 0.06 U/mg) secondly peroxidase activity of (0.34 ± 0.02 U/mg) and third catalase activity of (0.22 ± 0.01 U/mg) in different natural honey samples. The current study demonstrated that higher concentration of protein content and antioxidant enzymes were found in natural honey which means they are good source of antiox...

In Vitro Inhibitory Potential Of Honey Against α-Glucosidase Activity

2018

The health promoting characteristics of honey are mainly due to the presence of multiple metabolites including vitamins and essential minerals besides enzymes and co-enzymes. Honey is a valuable supplement for a healthy population as its benefits include “antioxidant”, “anti-inflammatory” and “antimicrobial” activities. The nutritional value of honey depends on the floral source of nectar, types of bees and geographical and climatic conditions of the environment. In the present investigation, three unifloral honeys from three different floral sources i.e. Helianthus (Sunflower), Citrus sinensis (Orange), and Punica granatum (Pomegranate) were investigated to ascertained for the potential inhibitory effect in vitro on key enzyme linked to type-2 diabetes (α glucosidase) in order to provide some possible mechanism by which it can be used in the management of the disease. The IC50 values of all the honeys analysed were in range from 308.780 to 767 (μg/ml). From the obtained IC50 values...

Recent Advances in the Discovery of Bioactive Components from Natural Honey

Therapeutic Applications of Honey and its Phytochemicals, 2020

Honey is one of the most valued natural products introduced to mankind since antiquity. Traditionally, honey is not only used as a food product but also as an alternative remedy for clinical conditions ranging from wound healing to cancer treatment. Honey contains about 200 beneficial bioactive constituents primarily comprising glucose and fructose and it also encompasses some vitamins, amino acids, minerals, and enzymes from fructo-oligosaccharides. Honey is an essential source of phenolic compounds and it is of great interest to see the amount and type of phenolic acids and flavonoids as they are responsible for nutraceutical properties as well as promising pharmacological functions such as antimicrobial, antidiabetic, anticancer, neuroprotective, cardioprotective, and wound healing properties. Additionally, several recent reports have also verified that the phenolic compound profile in honey is closely linked to the botanical and, often, the geographic origin of this food product. In this book chapter, therapeutic effects associated with the bioactive compounds in natural honey have been thoroughly discussed.

Pharmacological Insights and Therapeutic Potentials of Honey; An Updated Review

https://www.ijhsr.org/IJHSR\_Vol.12\_Issue.12\_Dec2022/IJHSR-Abstract13.html, 2022

A natural sweetener, honey has innumerable pharmacological properties in addition to its nutritive value. Honey has been in human use since 8000 years and has been traditionally used by almost all systems of medicine including Islamic medicine. Although chemical composition varies according to the botanical source, carbohydrates constitute a major portion of its composition. Fructose is in abundance followed by glucose. There are many other constituents present in small amounts like proteins, enzymes, amino acids, minerals, trace elements, vitamins, aromatic compounds and poly-phenols which contribute to various medicinal properties of honey. Honey has been in use traditionally for treatment of various ailments like gastritis, diarrhoea, ophthalmic conditions, skin infections, wounds etc. Enough evidence is now present supporting antimicrobial, antioxidant, wound healing and other potentials medicinal properties of honey. We systematically searched published papers on the subject in the databases of Web of Science, Scopus, PubMed, Science Direct, Crossref and Google Scholar between 1st January 2000 and July 2022 with an aim to review pharmacological and therapeutic potentials of honey. Owing to investigated pharmacological and therapeutic potentials of honey we conclude that honey can be looked upon as a safe and efficacious alternative in management and prevention of various disorders.

Phytochemistry, metabolism, and ethnomedical scenario of honey: A concurrent review

International Journal of Food Properties, 2017

Honey is a natural domestic sweet food material and a chemically diverse superfluous product of nectar acquired from flowers. Owing to the presence of higher amounts of antioxidant compounds covering phenolic, enzymes, organic acids, and bioactive peptides, it holds a strong ability to prevent various maladies. In addition, honey has also been exploited in certain cardiovascular, gastrointestinal, neoplastic, and inflammatory states along with its role in the treatment of copious infections and surgical wounds. Flavonoids and polyphenols are important bioactive components present in honey and have antioxidant properties. Some bioactive compounds, for example luteolin, galangin, isorhamnetin, quercetin, and kaempferol, are present in honey. Phenolic acids and flavonoids have important pharmacological activities such as anti-allergic, antibiotic , anticarcinogenic, and hypoglycaemic. Moreover, curative potential of bioactive components and their utilisation in value added food products are also the limelight of this article.

Biological Properties and Uses of Honey: A Concise Scientific review

Indian Journal of Pharmaceutical and Biological Research

The global prevalence of chronic diseases such as diabetes mellitus, hypertension, atherosclerosis, cancer and Alzheimer's disease is on the rise. These diseases constitute the major causes of death globally. Honey is a natural substance with many medicinal properties such as antibacterial, hepatoprotective, hypoglycemic, reproductive, and antihypertensive and antioxidant. This review presents findings that indicate honey may ameliorate oxidative stress in the gastrointestinal tract (GIT), liver, pancreas, kidney, reproductive organs and plasma/serum. Besides, the review highlights data that demonstrate the synergistic antioxidant effect of honey and antidiabetic drugs in the pancreas, kidney, and serum of diabetic rats. These data suggest that honey, administered alone or in combination with conventional therapy, might be a novel antioxidant in the management of chronic diseases commonly associated with oxidative stress. In view of the fact that the majority of these data emana...

Recent advances of honey in modern medicines: A review

Journal of Pharmacognosy and Phytochemistry, 2017

Honey is used frequently due to its nourishing and healing values. China, Turkey, Argentina, Ukraine, and United States are the main honey producing countries. The most imperative component of honey is carbohydrates. It is present in the form of monosaccharide, fructose and glucose. It plays a vital role as an antioxidant, anti-bacterial and anti-inflammatory agent. This amazing rich golden fluid is much better alternative to sugar. It is an instantaneous energy booster and is used in skin care, hair care and various health linked aspects. The role of honey has been accredited in literature and there is considerable evidence in support of its antioxidant and antiseptic nature, cough avoidance, fertility and wound healing characteristics. However, its use has been controversially deliberated and has not been sound acknowledged in the modern medicine. So the present review is to explore and highlight the role of honey in modern medicine.

Phytochemistry, Metabolism and Ethnomedical Scenerio of Honey: A Concurrent Review

International Journal of Food Properties, 2017

Honey is a natural domestic sweet food material and a chemically diverse superfluous product of nectar acquired from flowers. Owing to the presence of higher amounts of antioxidant compounds covering phenolic, enzymes, organic acids, and bioactive peptides, it holds a strong ability to prevent various maladies. In addition, honey has also been exploited in certain cardiovascular, gastrointestinal, neoplastic, and inflammatory states along with its role in the treatment of copious infections and surgical wounds. Flavonoids and polyphenols are important bioactive components present in honey and have antioxidant properties. Some bioactive compounds, for example luteolin, galangin, isorhamnetin, quercetin, and kaempferol, are present in honey. Phenolic acids and flavonoids have important pharmacological activities such as anti-allergic, antibiotic , anticarcinogenic, and hypoglycaemic. Moreover, curative potential of bioactive components and their utilisation in value added food products are also the limelight of this article.