Production and Purification of Highly Replication Defective HSV-1 Based Gene Therapy Vectors (original) (raw)

This thesis explores the production and purification of highly replication-defective HSV-1 gene therapy vectors, addressing key challenges in the field such as safety, large-scale manufacturing, and purification processes. The work highlights advances in downstream processing techniques, including optimized chromatography methods for different viral strains. The significance of replication-defective vectors is discussed in the context of various applications in gene therapy, not only for replacing missing gene functions but also as adjunct therapies for conditions like chronic pain and cancer, demonstrating their growing relevance in modern medical treatments.