Evaluation of Adaptability of Three Different Root Canal Sealers. (In Vitro Study) (original) (raw)
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Assessment of Marginal Adaptation and Sealing Ability of Root Canal Sealers: An in vitro Study
The Journal of Contemporary Dental Practice, 2017
Aim This study aims to compare the marginal adaptation and sealing ability [mineral trioxide aggregate (MTA)-Fillapex, AH Plus, Endofill sealers] of root canal sealers. Materials and methods In the present study, the inclusion criteria include 45 single-rooted extracted mandibular premolar teeth, with single canal and complete root formation. The sectioning of the samples was done at the cementoenamel junction using a low-speed diamond disc. Step-back technique was used to prepare root canals manually. The MTA-Fillapex, AH Plus, and Endofill sealers were the three experimental sealer groups to which 45 teeth were distributed. Under scanning electron microscope (SEM), marginal gap at sealer and root dentin interface were examined at coronal and apical halves of root canal. Results Among the three maximum marginal adaptations were seen with AH Plus sealer (4.10 ± 0.10) which is followed by Endofill sealer (1.44 ± 0.18) and MTA-Fillapex sealer (0.80 ± 0.22). Between the coronal and api...
International journal of scientific research, 2018
Aims: To compare the sealing ability of a new root canal sealer, Totalfill BC Sealer (FKG Dentaire SA, La Chaux-de-Fonds, Switzerland) with AH Plus Sealer (Dentsply, Tulsa, OK). Methods and Material: Sixty five permanent single rooted, human maxillary central incisors of similar sizes were selected for the study. Fifty teeth were randomly selected for dye penetration and fifteen teeth for Scanning Electron Microscopy. For dye penetration the teeth were divided into two groups of twenty five teeth each. In Group 1 (n=25), Totalfill BC Sealer and in Group 2 (n=25), AH Plus sealer were used as root canal sealers with Gutta-percha cones to obturate the canals. Roots were covered with nail paint except 2.0 mm of root apically and stored in 0.5% methylene blue dye for one week. The teeth were immersed in 7ml of 70% nitric acid for complete dissolution and subjected to UltraViolet spectrophotometer to quantify the concentration of the dye in each sample. For SEM evaluation, fifteen samples were sectioned longitudinally and marginal adaptation of sealers to root dentin was evaluated. Statistical analysis used: The dye leakage of both groups was analyzed using the Independent t-test. Marginal adaptation of sealers to root dentin was analyzed using unpaired student t test. Results:The mean dye leakage in Group 1 (0.36 ± 0.11) was less when compared to Group 2 (0.49 ± 0.12) (P<0.001 highly significant). The SEM study revealed gap-free regions and gap-containing regions in canals filled with both materials. Gap-containing regions in Group 1 were significantly low compared to Group 2. Conclusions: Within the limitations of this study, apical sealing ability and adaptation to the root canal dentin of Totalfill BC sealer is better compared to AH Plus sealer.
Medical review, 2017
Introduction. The aim of this in vitro study was to test and analyze the sealing ability of three endodontic materials used for permanent obturation, in between the dentin walls and the gutta-percha points, using a scanning electron microscope. Material and Methods. Forty-five recently extracted single-root teeth, treated by a step-back technique, were divided into three groups (15 teeth in each); the canals were filled with three different permanent obturation materi?als: N2 - zinc oxide root canal cement, Gutta Flow (Coltene), and Endomethasone N (Septodont). Their sealing ability and adhesive properties were analyzed using field emission gun scanning electron microscope, at the time when they were applied between the dentin walls of the canal and the gutta-percha. Results. The results of the scanning electron microscope analysis have shown that all the three sealers have good adhesion properties when used over the root canal walls in the apical third. Good adaptation of the filli...
Egyptian Dental Journal
Aim: This in vitro study assessed and compared two different root canal sealers with two different obturation techniques in terms of marginal adaptation using SEM Materials and Methods: Sixty extracted single-rooted lower premolar teeth with closed apices were collected. samples were then de-coronated, and chemo-mechanical preparation were performed. The samples were then equally divided into four main groups (n = 15) according to the root canal sealer and the obturation technique used. Group A: teeth were obturated by cold lateral condensation technique with AH-plus sealer. Group B: teeth were obturated by single cone obturation technique with AH Plus sealer. Group C: teeth were obturated by cold lateral condensation technique with Total fill BC root canal sealer. Group D: teeth were obturated by single cone obturation technique with Total fill BC root canal sealer. Specimens were then transversely sectioned. Marginal adaptation to the dentin of root canals was assessed at coronal, middle & apical thirds with the aid of scanning electron microscopy (SEM). Then, marginal gap scores were assessed. Result: The data were statistically analyzed by Two-way ANOVA and Three-way ANOVA tests to test the interactions between different variables. The lowest marginal gap value was observed in Group C while the highest marginal gap value was observed in Group B. Coronal thirds revealed superior marginal adaptation in comparison with middle and apical thirds within the different groups. Conclusions: Within the limitation of this study, bio-ceramic sealers are reliable and promising root canal sealers that markedly improve the marginal adaptation. Moreover, obturation techniques also had an impact on the marginal adaptation of the root canal sealer to the dentin walls of the root canal. Cold lateral compaction technique is considered a reliable technique that improves the adaptation to the root canal dentin walls.
Conservative Dentistry and Endodontic Journal, 2019
Aim: The aim of this study is to evaluate and compare the sealer penetrability and gap formation of root canal sealer to root dentin filled with AH Plus, Sealapex, and BioRoot RCS. Materials and methods: Twenty-seven mandibular second premolars were selected and were radiographed at two angulations. The teeth were stored in labeled plastic vials containing artificial saliva and were randomly assigned to three groups based on the sealer, group I-AH Plus (n = 9), group II-Sealapex (n = 9), and group III-BioRoot RCS (n = 9); teeth were de-coronated and the conventional root canal therapy was done with Protaper gold rotary files. Three groups were filled with AH Plus, Sealapex, and BioRoot RCS with the single-cone technique. About 1-mm sections of apical, middle, and cervical third were taken using a water-cooled low-speed saw. All specimens are evaluated using a scanning electron microscope. Clinical significance: The main goal of obturation is to provide a three-dimensional seal, thereby preventing the reinfection of the root canal and preserving the health of periapical tissues. Because of the hydrophobic nature of gutta-percha, the sealer tends to pull away from gutta-percha on the setting. To overcome these drawbacks, new sealer systems have been introduced to enhance the sealing ability. Resin-based sealers have gained more popularity in recent years because these sealers penetrate deep into the dentinal tubules due to their better flowability, long setting time, and provide long-term dimensional stability. The resin-based sealer used in this study is the AH Plus. It is compared with the newly introduced bioceramic sealer BioRoot RCS for marginal adaptation. Results: It was found that the AH Plus group had a higher depth of sealer penetration than other groups and the BioRoot RCS group revealed a minimum gap formation than other groups of sealers evaluated in the study. Conclusion: The Bioceramic sealer revealed better sealer penetrability at the apical third and minimal gap formation compared to the epoxy resin-based and the calcium hydroxide-based sealer.
2023
Introduction: This study aimed to investigate the interfacial adaptation of CeraSeal and Bio-C Sealer in comparison to AH Plus using scanning electron microscope (SEM). Additionally, to compare penetration depth of CeraSeal and Bio-C Sealer with AH Plus using confocal laser scanning microscopy (CLSM). Materials and Methods: Sixty-six extracted single rooted human premolars were collected and randomly divided into three groups according to sealer type AH Plus, Bio-C Sealer and CeraSeal using single cone obturation technique. Then each group was divided into 2 subgroups (n=11), one of the subdivided groups was obturated, then samples were cut longitudinally to be evaluated for interfacial adaptation using SEM at magnification 500x. For the other 33 samples, the sealer was mixed with Rhodamine B dye obturated, then the samples were cut horizontally at (3, 6 and 9 mm) to be evaluated for depth of penetration using CLSM. Results: For interfacial adaptation, AH Plus showed better adaptation without statistically significant difference between them. The maximum depth of penetration, Bio-C Sealer showed the maximum penetration, without statistically significant difference between them. While for penetration %, there was a statistically significant difference between the three sealers, with the Bio-C showed the best penetration percentage. Conclusions: Within the limitations of this study, it was concluded that AH Plus showed the best insignificant sealer adaptation to the root canal wall, while Bio-C sealer showed the most optimal dentinal tubular penetration of the tested sealers.
Evaluation of Sealing Ability of Three Root Canal Sealers: An In Vitro Study
The Journal of Contemporary Dental Practice
Aim: To evaluate the sealing ability of three different types of sealers using confocal laser microscopy. Materials and methods: Sixty extracted single-root premolars were selected and divided into three groups (20 teeth in each group) according to the type of sealer used, namely, mineral trioxide aggregate (MTA) Fillapex, AH Plus, and Bio C Sealer. Root canal preparation and obturation will be done in all the samples. Roots will be dissected transversely in apical plane. Percentage of gap from region to canal circumference will be calculated using a confocal laser microscope. Samples were subjected to statistical analysis. Results: High dye penetration was seen with AH Plus compared to MTA Fillapex and least with Bio C Sealer. The AH Plus is the best sealer with respect to seal ability of all the three. Conclusion: This study helps to appraise the sealing ability of the different types of sealers using confocal laser microscopy which is useful for the success of root canal treatment. Clinical significance: As sealer has to seal voids, foramina, and canals, it should have good penetration for the success of root canal treatment.
Journal of Liaquat University of Medical & Health Sciences
June-December 2018. Fresh extracted 32 teeth were included and their crowns were sectioned at Cemento-Enamel Junction/service to obtain 12mm standard root length. The access cavity was gained using endodontic instruments. The teeth were divided into two groups, in Group A (n=16) MTA Bioceramic and in Group B (n=16) AH Plus was used as root canal sealers by single cone obturation technique. The obturated specimens were stored in humid conditions for one week. Analysis was done using SPSS version 20. RESULTS: Mean and standard deviation of dye leakage for Bioceramic were 2.25±0.9 whereas for AH Plus was significantly lower, 1.19±0.75. Teeth included in Group A were central incisors 37.5%, lateral incisors 18.8%, canine 12.5%, lower first premolars 12.5% and second premolars 6.3%, whereas Group B consisted of central incisors 18.8%, lateral incisors 12.5 %, canine 12.5%, lower first premolars 18.8 % and second premolars 6.3%. Dye leakage was higher in Group A compared to Group B with a significant P-value < 0.001. CONCLUSION: It can be concluded based on this study that AH Plus offers a better apical seal as compared to Bioceramic-based MTA Fillapex sealers.
Open Journal of Stomatology, 2013
This study investigated the microleakage of two different root canal obturation systems, using the nuclear medicine approach, with sodium pertechnetate 99m Tc. Twenty six single-rooted extracted teeth were selected. The crowns were sectioned to obtain 15 mm long root segments and each tooth was prepared using rotary ProFile ® instruments. The roots were divided into 2 experimental groups and two control groups. Twenty root canals were filled, using Thermafil ® and Topseal ® or MTA Fillapex ® as a sealer. On the 7th and the 28th day the apices were submersed in a solution of 99m Tc-Pertechnetate during 3 hours. The radioactivity was counted using a gamma camera. Although apical leakage on the 7th day in the Topseal group was reduced compared with RealSeal1, with a statistical significant difference (p = 0.057), on the 28th day, the MTA Fillapex increased the sealing properties (p = 0.017).
COMPARISON OF THREE BIOCERAMIC SEALERS IN TERMS OF DENTINAL SEALING ABILITY IN THE ROOT CANAL
International Journal of Applied Pharmaceutics , 2020
Objective: The main objective of endodontic treatment is to prevent further infection or reinfection by eliminating microorganisms within the root canal system. Proper endodontic treatment could prevent apical and coronal penetration of fluids and microorganisms. Endodontic sealer is vital components of root canal obturation to establish a fluid-tight seal. Bioceramic-based root canal sealers are considered to be an advantageous technology in endodontics and have been found to be both biocompatible and comparable to other commercial sealers. The aim of this study was to compare the adhesion of three bioceramic sealers within the root canal system. Methods: Endodontically treated teeth were obturated using three types of bioceramic sealers and then divided into three groups. Specimens were then observed using a scanning electron microscope, and the attachment distance was measured using ImageJ. Results: The three groups exhibited were statistically significant differences (p<0.05) in dentinal sealing ability. Calcium phosphate silicate-based sealer showed the highest sealing ability, followed by pure tricalcium silicate-based bioceramic sealers and then tricalcium silicate and resin-based bioceramic sealers. Conclusion: The sealing ability of calcium phosphate silicate-based sealer is superior to that of both pure tricalcium silicate-based and tricalcium silicate-and resin-based sealer.