Desarrollo de una plataforma molecular para la detección y cuantificación del virus vacunal de Newcastle (original) (raw)
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REDVET, 2009
Análisis molecular de una cepa de virus de Newcastle de origen vacunal aislada a partir de un hisopado cloacal de aves sanas en Costa Rica http://www.veterinaria.org/revistas/redvet/n111109/111105.pdf 1 REDVET Rev. electrón. vet. http://www.veterinaria.org/revistas/redvet -http://revista.veterinaria.org Vol. 10, Nº 11, Noviembre/2009 -http://www.veterinaria.org/revistas/redvet/n111109.html Análisis molecular de una cepa de virus de Newcastle de origen vacunal aislada a partir de un hisopado cloacal de aves sanas en Costa Rica -Molecular analysis of an isolated Newcastle disease virus strain obtained from cloacal swabs in healthy poultry farm in Costa Rica
CEDAMAZ REVISTA UNL, 2017
Newcastle disease virus is a highly conta-gious avian paramyxovirus and the main de-vastating poultry agent, causing high econo-mic losses in the poultry industry around the world. In the present study, we characterized molecularly 8 partial gene sequences of the fu-sion protein and 3 complete sequences of the haemagglutinin-neuraminidase from isolated virus present in native poultry unvaccinated in the Southern Ecuador in 2014. These sequences were compared with other sequences of virus, representing different genotypes and sub-ge-notypes of virus from different regions of the world. All viruses showed the amino acid se-quence 112GRQGRL117 in C-terminus extension of the F2 protein, and showed Leucine at the N-terminus extension of the F1 protein. Those sequences allowed us to determine that these viruses belonged to a type of lentogenic virus. Phylogenetic analysis showed that most of the Newcastle virus isolated in this region of Ecua-dor, were closely related to strains of genotype II Class II, consistent with the sequence lento-genic live-virus vaccine, La Sota and B1. Mole-cular characterization and phylogenetic study of the F and HN genes could reliably be used as a tool for monitoring and early detection of Newcastle disease virus, as well as to predict the different pathotypes of virus isolates.Palabras clave: Análisis filogenético; Proteína de fusión; Proteína hemaglutinina-neuraminidasa; Virus de la enfermedad de Newcastle.Keywords: Phylogenetic analysis; Fusion protein; Hemagglutinin-neuraminidase protein; Newcastle Disease Virus.INTRODUCCIÓN
2012
The aim of the present study was the molecular characterization of Newcastle disease virus (NDV) isolates from domestic poultry in different regions of Perú using reverse transcription chain reaction polymerase (RT-PCR) test and fusion protein sequencing. Clinical samples from fighting cook, backyard and commercial poultry were collected from outbreaks between 2009 to 2011 in Perú. The RT-PCR tests detected the Newcastle disease virus in whole clinical samples, the viruses were characterized as high pathogenic NDV strains in backyard and fighting cook; and low pathogenic NDV strains in backyard and commercial poultry. To assess the virulence of NDV isolated was amplified and sequenced the fusion protein cleavage site and was analyzed phylogenetically in order to determine the pathotype. The virulent viruses showed the sequence 113 RQKRF 117 and phenylalanine at residue 117; which is characteristic of velogenic virulent strains, whereas low virulence strains showed basic amino acid at position 113-116 and leucine at residue 117; which is characteristic of lentogenic NDV strains. The virulent NDV strains belong to genotype VII and they are closely related with virulent NDV from Peru and Malasya. The lentogenic NDV strains are grouped into genotypes I and II, both of them are very related with United States (U.S.) NDV strains.
Revista de Investigaciones Veterinarias del Perú, 2014
Sera from 255 apparently nonnal birds pertaining to 16 species ofthe Psittacifonnes order, at Parque de las Leyendas Zoo in Lima were tested for the presence ofNewcastle Disease antibodies. The hemagglutination-inhibition test was used for detecting antibodies against Paramyxovirus serotype 1. Ali ofthe birds tested negative, suggesting that they have not been exposed to the Newcastle Disease virus and <lid not comprise a Paramyxovirus-1 reservoir at the moment that this study was done.
Revista de Investigaciones Veterinarias del Perú, 2020
El presente estudio evaluó la eficacia de una vacuna vectorizada contra el virus de la enfermedad de Newcastle (vENC) aplicada en planta de incubación. Doscientos pollitos BB fueron distribuidos en cuatro grupos experimentales de vacunación contra vENC: T1: Vacuna viva atenuada + vacuna inactivada, T2: Vacuna viva atenuada, T3: Vacuna viva atenuada + vacuna vectorizada, T4: Grupo control no vacunado. Todas las aves fueron, además, vacunadas contra el virus de Bronquitis infecciosa, Marek y Gumboro el día 1. Las aves fueron desafiadas el día 26 con una cepa velogénica de campo altamente patógena. Se evaluó la mortalidad, signos clínicos pos-desafío, lesiones en la necropsia y respuesta de anticuerpos contra vENC. Las aves vacunadas con la vacuna vectorizada de vENC presentaron una menor mortalidad (2%) en comparación a las aves vacunadas con cepas vivas atenuadas (12.5% para T1 y 18.4% para T2, respectivamente; p<0.05). Asimismo, menor porcentaje de manifestaciones clínicas pos-de...
…, 2009
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