TaqMan qPCR for Quantification of Clonostachys rosea Used as a Biological Control Agent Against Fusarium graminearum (original) (raw)

This study presents the development of a quantitative real-time PCR (qPCR) assay using TaqMan chemistry for the detection and quantification of Clonostachys rosea, a biological control agent against Fusarium graminearum. The assay targets the actin gene, demonstrating high sensitivity and specificity, which allows for detection of as few as 100 genome copies per reaction. It effectively monitors C. rosea in various sample matrices throughout the barley malting process, contributing to sustainable disease control strategies in agriculture.