BIOETHANOL PRODUCTION USING Saccharomyces cerevisiae IMMOBILISED ON FRESH AND MODIFIED SUGARCANE BAGASSE (original) (raw)
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Pretreated of sugarcane bagasse was used as a carrier for immobilization of Saccharomyces cerevisiae. Pretreatments were carried out by steaming, pressurized steam, and combination both of procedure. The objectives of this research was to investigate the effect of pretreatment on sugarcane bagasse to cells adsorption and bioethanol production. Immobilization process was conducted in a ratio of 2.5 g carrier/50 mL cell suspension. Whole cell biocatalyst as much as 1% (w/v) was used as inoculum for bioethanol fermentation. The best pretreated sugarcane bagasse for carrier of immobilized cells was obtained using steam treatment for 30 minutes. Those treatment improved the physical properties of carrier and increased the cell retention up to 10.05 mg/g. The use of whole cell biocatalyst after steaming pretreatment also enhanced ethanol yield 1.5 times higher than control. Keywords: bioethanol; immobilization; pretreatment; steam treatment; sugarcane bagasse
Renewable Energy, 2013
Microwave alkali pretreated sugarcane bagasse was used as a substrate for production of cellulolytic enzymes, needed for biomass hydrolysis. The pretreated sugarcane bagasse was enzymatic hydrolyzed by crude unprocessed enzymes cellulase (Filter paper activity 9.4 FPU/g), endoglucanase (carboxymethylcellulase, 148 IU/g), b-glucosidase (116 IU/g) and xylanase (201 IU/g) produced by Aspergillus flavus using pretreated sugarcane bagasse as substrate under solid state fermentation. Concentrated enzymatic hydrolyzate was used for ethanol production using Saccharomyces cerevisiae immobilized on various matrices. The yield of ethanol was 0.44 g p /g s in case of yeast immobilized sugarcane bagasse, 0.38 g p /g s using Ca-alginate and 0.33 g p /g s using agar-agar as immobilization matrices. The immobilized yeast studied up to 10 cycles in case of immobilized sugarcane bagasse and up to 4 cycles in case of agaragar and calcium alginate for ethanol production under repeated batch fermentation study.
Makara Journal of Technology, 2016
Sugarcane bagasse was used as a carrier to immobilize Saccharomyces cerevisiae in bioethanol production. This research aims to study the potential use of sugarcane bagasse as an alternative carrier for cell immobilization and improvement in the production process of cell immobilization in bagasse. The results showed that the physical characteristics of sugarcane bagasse as a carrier were water content (7.77 ± 0.35%), water retention (4.80 ± 0.44 g/g), water absorption index (8.58 ± 0.22 g/g), and lignin content (24.40 ± 1.52 %). Determination of cell retention was performed in an inoculum volume of 50 mL yeast suspension with various carrier weights (2.5, 5, 10, and 20 g). The highest cell retention was obtained in ratio of 2.5 g carrier/50 mL cell suspension with cell retention of 5.41 ± 1.06 mg/g, or known as biocatalyst. Biocatalyst, as much as 1.5, 3, 4.5, and 6 g, were used as inoculum for a 24 hour bioethanol fermentation. The best concentration and productivity of bioethanol, obtained by using 3 g of biocatalyst, were 23.95 ± 0.28 g/L and 1.24 ± 0.01 g/L/hours. The average of bioethanol yield for a 24 hour fermentation by using immobilized cells was three times higher than the free cells system. Abstrak Bagas sebagai Carrier untuk Imobilisasi Saccharomyces cerevisiae pada Produksi Bioetanol. Bagas digunakan sebagai carrier untuk imobilisasi pada produksi bioetanol. Penelitian bertujuan untuk mengetahui potensi penggunaan bagas sebagai carrier alternatif untuk imobilisasi sel dan perbaikan proses pembuatan imobilisasi sel pada bagas. Hasil penelitian menunjukkan bahwa karakteristik fisik yang dimiliki bagas sebagai carrier yaitu kadar air (7,77 ± 0,35%), retensi air (4,80 ± 0,44 g/g), indeks penyerapan air (8,58 ± 0,22 g/g), dan kadar lignin (24,40 ± 1,52%). Pengukuran retensi sel dilakukan pada variasi berat carrier (2,5, 5, 10, dan 20 g) dengan volume inokulum sebanyak 50 mL suspensi sel. Retensi sel tertinggi, diperoleh pada perbandingan 2,5 g carrier/50 mL suspensi sel, yaitu 5,41 ± 1,06 mg/g dan disebut sebagai biokatalis. Biokatalis sebanyak 1,5; 3; 4,5; dan 6 g digunakan sebagai inokulum untuk fermentasi bioetanol selama 24 jam. Konsentrasi dan produktivitas bioetanol terbaik menggunakan 3 g biokatalis yaitu 23,95 ± 0,28 g/L dan 1,24 ± 0,01 g/L/jam. Rendemen bioetanol selama 24 jam fermentasi menggunakan sel terimobilisasi adalah tiga kali lebih tinggi dibandingan dengan sel bebas.
Biotechnology and Applied Biochemistry, 2014
Continuous ethanol fermentation using polyvinyl alcohol (PVA), immobilized yeast, and sugarcane molasses (22 and 35 • Bx) with 8 g/L urea was run in a combined bioreactor system consisting of three-stage tubular bioreactors in series. The effect of the dilution rate (D) at 0.0037, 0.0075, 0.0117, 0.0145, 0.018 , and 0.0282 H −1 on continuous ethanol fermentation was investigated in this study. The results showed that D had a significant effect on fermentation efficiency, sugar-utilized rate, ethanol yield, and ethanol productivity in this designed continuous fermentation system. The D had a linear relationship with residual sugar and ethanol production under certain conditions. The highest fermentation efficiency of 83.26%, ethanol yield of 0.44 g/g, and the lowest residual sugar content of 6.50 g/L were achieved at 0.0037 H −1 in the fermentation of 22 • Bx molasses, indicating that the immobilization of cells using PVA, sugarcane pieces, and cotton towel is feasible and the established continuous system performs well.
Mycobiology, 2012
A repeated batch fermentation system was used to produce ethanol using Saccharomyces cerevisiae strain (NCIM 3640) immobilized on sugarcane (Saccharum officinarum L.) pieces. For comparison free cells were also used to produce ethanol by repeated batch fermentation. Scanning electron microscopy evidently showed that cell immobilization resulted in firm adsorption of the yeast cells within subsurface cavities, capillary flow through the vessels of the vascular bundle structure, and attachment of the yeast to the surface of the sugarcane pieces. Repeated batch fermentations using sugarcane supported biocatalyst were successfully carried out for at least ten times without any significant loss in ethanol production from sugarcane juice and molasses. The number of cells attached to the support increased during the fermentation process, and fewer yeast cells leaked into fermentation broth. Ethanol concentrations (about 72.65~76.28 g/L in an average value) and ethanol productivities (about 2.27~2.36 g/L/hr in an average value) were high and stable, and residual sugar concentrations were low in all fermentations (0.9~3.25 g/L) with conversions ranging from 98.03~99.43%, showing efficiency 91.57~95.43 and operational stability of biocatalyst for ethanol fermentation. The results of the work pertaining to the use of sugarcane as immobilized yeast support could be promising for industrial fermentations.
Bioresource Technology, 2009
Saccharum spontaneum is a wasteland weed consists of 45.10 ± 0.35% cellulose and 22.75 ± 0.28% of hemicellulose on dry solid (DS) basis. Aqueous ammonia delignified S. spontaneum yielded total reducing sugars, 53.91 ± 0.44 g/L (539.10 ± 0.55 mg/g of substrate) with a hydrolytic efficiency of 77.85 ± 0.45%. The enzymes required for hydrolysis were prepared from culture supernatants of Aspergillus oryzae MTCC 1846. A maximum of 0.85 ± 0.07 IU/mL of filter paperase (FPase), 1.25 ± 0.04 IU/mL of carboxy methyl cellulase (CMCase) and 55.56 ± 0.52 IU/mL of xylanase activity was obtained after 7 days of incubation at 28 ± 0.5°C using delignified S. spontaneum as carbon source under submerged fermentation conditions. Enzymatic hydrolysate of S. spontaneum was then tested for ethanol production under batch and repeated batch production system using ''in-situ" entrapped Saccharomyces cerevisiae VS 3 cells in S. spontaneum stalks (1 cm  1 cm) size. Immobilization was confirmed by the scanning electron microscopy (SEM). Batch fermentation of VS 3 free cells and immobilized cells showed ethanol production, 19.45 ± 0.55 g/L (yield, 0.410 ± 0.010 g/g) and 21.66 ± 0.62 g/L (yield, 0.434 ± 0.021 g/g), respectively. Immobilized VS 3 cells showed maximum ethanol production (22.85 ± 0.44 g/L, yield, 0.45 ± 0.04 g/g) up to 8th cycle during repeated batch fermentation followed by a gradual reduction in subsequent cycles of fermentation.
2012
A repeated batch fermentation system was used to produce ethanol using Saccharomyces cerevisiae strain (NCIM 3640) immobilized on sugarcane (Saccharum officinarum L.) pieces. For comparison free cells were also used to produce ethanol by repeated batch fermentation. Scanning electron microscopy evidently showed that cell immobilization resulted in firm adsorption of the yeast cells within subsurface cavities, capillary flow through the vessels of the vascular bundle structure, and attachment of the yeast to the surface of the sugarcane pieces. Repeated batch fermentations using sugarcane supported biocatalyst were successfully carried out for at least ten times without any significant loss in ethanol production from sug-arcane juice and molasses. The number of cells attached to the support increased during the fermentation process, and fewer yeast cells leaked into fermentation broth. Ethanol concentrations (about 72.65~76.28 g/L in an average value) and ethanol productivities (abou...
2017
Sugarcane bagasse (SCB) is most abundant agricultural wastes in the world. It is an attractive feedstock for the large-scale biological production of bioethanol. However, the limitation in bagase use is its high degree of complexity because of its mixed composition of extremely inhomogeneous fibers. Therefore, ethanol production from bagase is often complex, with three main steps, i.e pretreatment, sacharification, and fermentation. Here we used alkali pretreatment using delignification reactor with NaOH 1N and 1.5 bar for 2 hours. Followed by Simultaneous Sacarification and Fermentation (SSF) using Saccharomyces cerevisiae in addition of cellulase and β-glucosidase enzyme. We found that the alkaline pretreatment can decrease cellulose crystallinity, decrease lignin content up to 84.83% and increased cellulose content up to 74.29%. SSF using cellulase enzymes and combination of cellulase enzymes and β-glucosidase derived bioethanol levels respectively 5.87±0.78% and 6.83±0.07%. In conclusion these results strongly suggest that addition of β-glucosidase enzyme on alkali-pretreated bagasse increased the bioethanol production.