In vitro Antimicrobial Activity of Artemisia annua Leaf Extracts against Pathogenic Bacteria (original) (raw)
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Antibacterial and Phytochemical Screening of Artemisia Species
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Taking into account the increasing number of antibiotic-resistant bacteria, actual research focused on plant extracts is vital. The aim of our study was to investigate leaf and stem ethanolic extracts of Artemisia absinthium L. and Artemisia annua L. in order to explore their antioxidant and antibacterial activities. Total phenolic content (TPC) was evaluated spectrophotometrically. Antioxidant activity was evaluated by DPPH and ABTS. The antibacterial activity of wormwood extracts was assessed by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) in Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, and Salmonella enteritidis cultures, and by zone of inhibition in Klebsiella carbapenem-resistant enterobacteriaceae (CRE) and Escherichia coli extended-spectrum β-lactamases cultures (ESBL). The Artemisia annua L. leaf extract (AnL) exhibited the highest TPC (518.09 mg/mL) and the highest expression of sinapic acid (285.69 ± 0.002 µg/mL). ...
Antimicrobial effects and Phytoconstituents of ethanolic extract of leaves of Artemisia annua L.
Tel: +2348062685063] Studies were carried out on antimicrobial effects and phytoconstituents of Ethanolic extract of leaves of Artemisia annua L. The extract significantly inhibited the test organisms. The minimum inhibitory concentration value of the ethanolic extract was 125 mg/ml for each of the test organisms: Escherichia coli, Streptococcus faecalis, Pseudomonas auroginosa, Klebsiella pneumoniae, Candida albicans and Staphylococcus aureus. The result of the minimum bactericidal concentration showed that, the extract was bactericidal at the concentration of 250 mg/ml. The phytochemical analysis revealed the presence of Tannins, Flavonoids, Cardiac Glycosides, Alkaloids, Phenols and Resins.
Screening antimicrobial activity of various extracts of Artemisia dracunculus L
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The antimicrobial activities of chloroform, acetone and two different concentrations of methanol extracts of Artemisia dracunculus L. were studied. These extracts were tested against nine bacteria and four yeasts strains by the disc diffusion method. The results indicated that the methanol extract of A. dracunculus is more effective against tested microorganisms than chloroform or acetone extracts. The chloroform and acetone extracts were inhibitory only towards Pseudomonas aeruginosa (ATCC 27853). While the methanol extract that was diluted with 10 ml distilled water showed inhibition zones against Shigella (RSHI), Listeria monocytogenes ATCC 7644, P. aeruginosa (ATCC 27853), the methanol extract that was diluted with 5 ml distilled water showed inhibition zones against two different strains of Escherichia coli (RSHI, ATCC 25922), Shigella (RSHI), L. monocytogenes (ATCC 7644), and P. aeruginosa ATCC 27853. The cells of microorganisms treated with plant extracts and normal microorga...
Antimicrobial Activity of Leaves of Artemisia Vulgaris L
Proceedings of The 16th International Electronic Conference on Synthetic Organic Chemistry
Objective: To screen the antimicrobial activity of different extracts of leaves of Artemisia vulgaris. Materials and Methods: To detect the in vitro antibacterial activity, 10 bacterial strains were selected. These bacteria are both gram +ve and gram-ve. Leaves were extracted with a petroleum ether, chloroform, ethyl acetate, ethanol and aqueous. In the present work the antibacterial activity was done by cup plate method. The antibacterial activity was expressed as zone diameter in millimeters. Different extracts from leaves of the plant was compared with standards like benzyl penicillin for gram +ve bacteria and streptomycin for gram-ve bacteria using DMF as control. The readymade media for inoculum and culture was obtained from Himedia labs. For antifungal activity four fungal organisms were selected and Griseofulvin was used as standard. Results: Herbal extracts prepared from the leaves of the plant were screened against bacteria and fungal organisms at the concentration range between 50 µg and 300 µg/0.1ml. The results of antimicrobial activity revealed that the extract exhibited activity against both gram +ve, gram-ve and fungal organisms. Conclusion: The present investigation reveals that the aqueous, chloroform and ethyl acetate extracts and in some cases petroleum ether extract showed significant antimicrobial activity when compared with standard.
Anti-inflammatory and Antibacterial Activity of the Methanol Extract of Artemisia Herba-Alba
Egyptian Academic Journal of Biological Sciences. C, Physiology & Molecular Biology, 2022
The use of medicinal plants from the traditional African pharmacopoeia in the treatment of various diseases has been known for a long time, and the positive effects of this phytotherapy are no longer in question. However, these practices are based on empiricism. The methanolic extract of the leaves of Artemisia herba-alba has analgesic and anti-inflammatory properties that justify its traditional use. This study evaluates the anti-inflammatory and antibacterial properties of the methanolic leaf extract of Artemisia herba-alba. The negative control and test groups received 40 mg/kg of the extract. The anti-hematogenic effects of the extract showed a significant decrease in carrageenan-induced paw edema at 1 and 3 hours, as well as dextran-induced inflammation, compared to the control. In addition, there was inhibition of xylene-induced ear edema with a significant reduction compared to the control (p<0.05). The bacterial activity was determined on five bacterial strains: Klebsiella pneumoniae ATCC700603, Citrobacter freindii ATCC8090, Salmonella typhii ATCC13311, Enterobacter coloacae ATCC13047 and Staphylococcus aureus ATCC25923, according to the in vitro agar diffusion method (aromatogram). Our extract would therefore be an advantageous source of improved traditional medicine that is accessible and less expensive for people.
Antibacterial and antioxidant activities of Artemisia annua essential oil
Fitoterapia, 2002
The essential oil of Artemisia annua aerial parts, consisting of camphor (44%), germacrene D (16%), trans-pinocarveol (11%), b-selinene (9%), b-caryophyllene (9%) and artemisia ketone (3%), was screened for its antimicrobial activity. The essential oil remarkably inhibited the growth of tested Gram-positive bacteria Enterococcus hirae and both tested fungi. This oil has shown an antioxidant activity equivalent to 18% of the reference compound (atocopherol). ᮊ
ACTA Pharmaceutica Sciencia
Recently, most researches have focused on the biological activities of the extracts obtained from different Artemisia species due to the presence of essential compounds with strong activity against some gram-negative and gram-positive bacteria. In this study, five extracts of Artemisia rutifolia Stephan ex Spreng. from the northeastern Gilgit-Baltistan region of Pakistan were analyzed for total flavonoid and total phenolic contents and their antibacterial activities against some clinical and phyto-pathogenic bacterial strains were assessed with agar disk diffusion method. Results indicated that the methanol, ethanol, chloroform, ethyl acetate and n-hexane extracts of A. rutifolia are rich in flavonoids and phenols and all the tested extracts showed the broad spectrum growth inhibition of the tested gram positive (Bacillus subtilis and Staphylococcus aureus) and gram negative bacterial strains (Escherichia coli and Pseudomonas aeruginosa). Overall, methanol and ethyl acetate extracts showed better activities even at lower concentrations (5 mg/
Jorjani Biomedicine Journal, 2020
Background and objective: Some common problems in the health care system are Microbial resistance to antibiotics, the side effects of food additives, and preservatives. Considering the antibiotic resistance of microorganisms and the need to identify new compounds, the present study was conducted to determine the antimicrobial effects of Artemisia extracts. Material And Method The study was performed in two stages including extraction and determination of antibacterial properties of aqueous, ethanolic, methanolic, acetone/ethanolic, and hydroethanolic extracts of Artemisia on standard Pseudomonas aeruginosa, Streptococcus pyogenes, and oral bacteria sample. The well diffusion method was performed to evaluate the concentration that had an inhibitory and bactericidal effect. Result: Aqueous extract had an effect on the oral bacteria sample (8 mm), the methanolic extract had an effect on S. pyogenes and oral bacteria sample (15 mm) and finally acetone/ethanolic extracts had antimicrobial properties against S. pyogenes (16 mm). The concentration used for all five extracts was 50 mg/ml and showed an inhibition effect on the growth of S. pyogenes standard strain and oral bacteria sample. The less serial dilutions of extracts were tested but no antibacterial effects were seen. So, 50 mg/ml was the minimum concentration that had an inhibitory and bactericidal effect. Conclusion: It can be inferred that aqueous, methanolic, and acetone/ethanolic extracts of Artemisia had the highest inhibitory effect on S. pyogenes and the oral bacteria sample. Consequently, by applying different extraction methods and by utilizing different solvents, it may be possible to more efficiently obtain biomaterials with antimicrobial properties from this plant.