Isolation of a Novel Cytokine From Human Fibroblasts That Specifically Inhibits Osteoclastogenesis (original) (raw)

ment, and osteoblastic stromal cells (6, 7). In vivo stud-A factor which inhibits osteoclast-like cell formation ies on op/op mice bearing a mutation in macrophagewas found in the conditioned medium of human emcolony stimulating factor (M-CSF) gene demonstrated bryonic lung fibroblasts, IMR-90. The factor, termed that M-CSF is one of the factors responsible for the osteoclastogenesis inhibitory factor, OCIF, was puridifferentiation of both osteoclasts and monocytes/macfied to homogeneity. OCIF is a heparin-binding basic rophages (8-10). Similarly, it has been suggested that glycoprotein and has been isolated as a monomer with interleukin (IL)-6-mediated stimulation of osteoclastoan apparent molecular weight (Mr) of 60,000 and a hogenesis after estrogen loss accelerates bone resorption modimer with a Mr of 120,000. The N-terminus of OCIF in postmenopausal osteoporosis (11).