Analysis of oxidative stress in Wistar rats submitted to high- intensity interval training (original) (raw)
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Endurance training attenuates the oxidative stress due to acute exhaustive exercise in rat liver
Acta Physiologica Hungarica, 2008
The aim of this study was to investigate whether an 8-week treadmill training attenuates exerciseinduced oxidative stress in rat liver. Male rats were divided into untrained and trained groups. Endurance training consisted of treadmill running at a speed of 2.1 km/h, 1.5 h/day, 5 days a week for 8 weeks. To see the effects of endurance training on acute exhaustive exercise induced oxidative stress, untrained and trained rats were further devided into two groups: animals killed at rest and those killed after acute exhaustive exercise, in which the rats run at 2.1 km/h (10% uphill) until exhaustion. Acute exhaustive exercise increased malondialdehyde level in untrained but not in trained rats. It decreased the activity of glutathione peroxidase and total (enzymatic plus non-enzymatic) superoxide scavenger activity in untrained rats and catalase activity in trained rats. However, it did not affect glutathione S-transferase, glutathione reductase, superoxide dismutase and non-enzymatic superoxide radical scavenger activities in both trained and untrained rats. On the other hand, endurance training decreased glutathione peroxidase and glutathione S-transferase activities. The results suggested that endurance training attenuated exercise-induced oxidative stress in liver, probably by preventing the decreases in glutathione peroxidase and total superoxide scavenger activities during exercise.
Motricidade, 2020
Physical exercise is a known factor that can promote oxidative stress and may result in cellular damage if not neutralized by antioxidant mechanisms. The aim of this study was to determine if the level of hepatic oxidative stress resulting from high-intensity interval training (HIIT) is affected by the frequency pattern (consecutive vs. non-consecutive) of the training sessions. Thirty-two Wistar rats were divided equally into four groups: two control groups, (CS1) and (CS2), remained sedentary throughout the experiment, and two test groups, (CT1) and (CT2), and were submitted to HIIT for 12 consecutive and nonconsecutive (12 sessions, 3 times/wk over four weeks) days, respectively. There were no significant differences in markers of oxidative damage measured in hepatic tissue (TBARS) and markers of antioxidant activity (Sulfhydryl, FRAP), as well as markers of hepatic damage (AST and ALT) and antioxidant defense (Uric Acid) measured in plasma of both HIIT groups after training comp...
Influence of exercise training frequency on cardiac and hepatic oxidative stress in rats
Experimental and clinical cardiology, 2003
The present study investigated the influence of different frequencies of moderate exercise (13 weeks of treadmill running at 60% of maximal oxygen consumption) on oxidative stress in the heart and liver in rats. Oxidative stress was evaluated by chemiluminescence and lipid peroxidation (LPO) through thiobarbituric acid reactive substances. Activities of superoxide dismutase (SOD), glutathione peroxidase (GHPx) and catalase (CAT) were also measured. The animals were divided into four groups: control (C), acute ([A], only one exercise session at the end of 13 weeks), low frequency ([LF], one session a week for 13 weeks) and high frequency ([HF], five sessions a week for 13 weeks). Chronic exercise promoted cardiac hypertrophy in the HF group. Myocardial LPO in groups A and LF was increased, whereas in the HF group, it was decreased when compared with group C. The HF group demonstrated decreased myocardial SOD and GHPx activities and increased CAT activity. All exercise groups exhibite...
Chronically and acutely exercised rats: biomarkers of oxidative stress and endogenous antioxidants
Journal of Applied Physiology
The responses to oxidative stress induced by chronic exercise (8-wk treadmill running) or acute exercise (treadmill running to exhaustion) were investigated in the brain, liver, heart, kidney, and muscles of rats. Various biomarkers of oxidative stress were measured, namely, lipid peroxidation [malondialdehyde (MDA)], protein oxidation (protein carbonyl levels and glutamine synthetase activity), oxidative DNA damage (8-hydroxy-2′-deoxyguanosine), and endogenous antioxidants (ascorbic acid, α-tocopherol, glutathione, ubiquinone, ubiquinol, and cysteine). The predominant changes are in MDA, ascorbic acid, glutathione, cysteine, and cystine. The mitochondrial fraction of brain and liver showed oxidative changes as assayed by MDA similar to those of the tissue homogenate. Our results show that the responses of the brain to oxidative stress by acute or chronic exercise are quite different from those in the liver, heart, fast muscle, and slow muscle; oxidative stress by acute or chronic e...
Food and Chemical Toxicology, 2013
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Free Radical Research, 2006
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Impact of High-Intensity Exercise on Antioxidant System and Liver Enzymes
Pakistan BioMedical Journal
Liver is a vital organ of human body performing a variety of functions. In addition, maintenance of antioxidant system is also considered important. Objective: To observe the impact of high volume and high intensity exercise on blood redox state and enzymatic function of the liver. It was a comparative cross-sectional study conducted from January 2017 to Jan 2018 at Gomal University, Dera Ismail Khan Pakistan Methods: An observational study was conducted among twenty (40) voluntarily selected subjects. Measurement of both enzymatic functions of liver and blood redox state of the body were performed through liver functions tests (LFTS) and ferric reducing assay protocol (FRAP assay) by using blood samples collected from the subjects. Results: The collected data were analyzed through statistical package for social sciences (SPSS) version 25 by using different statistical tools such as Mean, Standard Deviation and T Score. Conclusions: Data analysis disclosed that high intensity exerci...
Advances in clinical and experimental medicine : official organ Wroclaw Medical University
Exercise induces a multitude of physiological and biochemical changes in blood that can affect its redox status. Exercise causes an increase in oxygen consumption by the whole body and particularly by the exercising muscle. As a result of this process there is a rise in the production of reactive oxygen species (ROS), which are capable to trigger a chain of damaging biochemical and physiological changes known as lipid peroxidation and oxidative stress. Since the early work of Dillard et al. in 1978, and findings of increased lipid peroxidation following acute aerobic exercise, the topic of exercise-induced oxidative stress has received considerable attention. The aim of this study was to examine how swimming to the point of exhaustion affects oxidative stress generation and nonenzymatic antioxidant activity in an animal model. The experiments were conducted on 10 male Dunkin-Hartley guinea pigs, and the swimming program used in the study, described as swimming to exhaustion, was def...
Antioxidants, Tissue Damage, and Endurance in Trained and Untrained Young Male Rats
Archives of Biochemistry and Biophysics, 1996
the greater antioxidant level should allow trained muscle to withstand oxidative processes more effec-It is well known that physical training permits an tively, thus lengthening the time required so that the animal to respond successfully to exercise loads of varcell function is sufficiently damaged as to make furious types, intensities, and durations. Furthermore, ther exercise impossible. ᭧ 1996 Academic Press, Inc. the trained animal can sustain the activity for a long Key Words: exhaustive exercise; tissue damage; period before the fatigue becomes limiting. The effects training; antioxidants; endurance. of physical training on the antioxidant defenses of tissues and on their susceptibility to damage induced by exhaustive exercise have been investigated. Therefore, untrained rats were sacrificed either at rest or In recent years exercise has expanded in scope from immediately after swimming to exhaustion. Rats competitive sports to disease prevention and health trained to swim for 10 weeks were also sacrificed, 48 hr promotion. There is, indeed, convincing evidence supafter the last exercise, either at rest or after exhaustive porting the role of exercise in the prevention and/or swimming. Homogenates of liver, heart, and muscle management of certain chronic diseases (1). On the were used for biochemical determinations. Mitochonother hand, there is some evidence that exercise indrial and sarcoplasmic (SR) or endoplasmic (ER) reticcreases the free radical production (2, 3). The highly ulum integrity was assessed with measurements of resreactive free radicals are known to modify several critipiratory control index and latency of alkaline phosphatase activity. Lipid peroxidation was measured by cal cellular components such as DNA, proteins, and determination of malondialdehyde and hydroperox-membrane lipids, leading to tissue damage (4). The ides. Additionally, the effect of training on the antioxidamage induced by exercise is gradual and cumulative, dant protection systems of tissues was examined by depending mainly (at constant workload) on the work determining the glutathione peroxidase and glutathiduration (2).