Micro Total Analysis System Based on Magnetic Nanoparticles for Allergy Diagnosis (original) (raw)
We report on the development of a microdevice dedicated to allergy diagnosis which combines both the advantages of homogeneous and heterogeneous immunoassays (IAs). As allergy diagnosis is based on IgE quantitation, we have used magnetic core shell nanoparticles (MCSNPs) as IgE capture nanoplateform. Integrating such immunosupport allows to perform the target antibody (IgE) capture in colloidal phase and thus to increase the IgE capture kinetics. Moreover, due to their magnetic properties and to the magnetic trap we have previously developed, the MCSNPs and therefore the target will be confined and preconcentrated prior the detection step. This microfluidic approach has been successfully developed for allergy diagnosis. The assay time was 20 min with a IgE limit of detection of 1 ng/mL.
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