Microvessel Density among the Morphological Types of Multiple Myeloma (original) (raw)
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Prognostic significance of plasma cell morphology in multiple myeloma
Cancer, 1987
The effect of bone marrow plasma cell morphology at diagnosis on survival time was evaluated in 139 patients with multiple myeloma. According to the morphological classification scheme the patients were categorized as mature (30 patients), immature (76 patients) or ...
JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH, 2021
Introduction: Cytogenetics has become an integral part of Multiple Myeloma (MM) diagnosis and prognostication. A combination of conventional cytogenetics and interphase Fluorescence In Situ Hybridization (FISH) is currently used to stratify tumours into high, intermediate and standard risk disease. Aim: To compare the morphological details of plasma cells with cytogenetic abnormalities. Materials and Methods: The present retrospective cross sectional study was conducted at Department of Pathology Dayanand Medical College and Hospital, Ludhiana in three and a half year duration (1st January 2014 to 30th June 2017). All the diagnosed MM patients in whom cytogenetic was available were included and descriptive analysis was done using Chi-Square test and relevant statistical analysis using SPSS 21 version. Correlation was done with various morphological pattern (plasmacytic, plasma blastic). Results: Cytogenetic studies were performed on 42 cases using FISH technique (n=31, 81.6%) and GT...
Diagnostic Pathology, 2010
Background: Estimation of plasma cell infiltrates in bone marrow aspirates (BMA) and bone marrow biopsy (BMB) is a standard method in the diagnosis and monitoring of multiple myeloma (MM). Plasma cell fraction in the bone marrow is therefore critical for the classification and optimal clinical management of patients with plasma cell dyscrasias. The aim of the study was to compare the percentage of plasma cells obtained by both methods with the patient clinical parameters and survival.
American Journal of Hematology, 2006
We studied the prognostic value of parameters of angiogenesis on bone marrow biopsies in newly diagnosed multiple myeloma (MM) patients. Angiogenesis parameters studied were the microvessel count done manually on light microscopy (MVD-A), microvessel count done by using computerized image analyzer (MVD-B), and total vascular area (TVA) measured by computerized image analyzer. One hundred ten newly diagnosed cases of MM treated at Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, were analyzed with respect to clinical features, laboratory findings, histological features, angiogenesis parameters, and responses to the treatment on follow-up. Twenty age-and sex-matched controls were studied for comparing with angiogenesis of the test cases. Bone marrow microvessels were examined using immunohistochemical staining for CD34. MVD-A (range 4.9-85.2; mean 28.2; SD 19.4), MVD-B (range 2.0-26.9; mean 11.7; SD 5.9), and TVA measured in percentage (range 0.1-17.1; mean 2.4; SD 2.5) were measured for test cases (n = 110). Grading of angiogenesis parameters of the test cases were done; such that angiogenesis parameters of controls (taken as baseline) were grade I. There was a statistically highly significant correlation between (MVD-A vs MVD-B, pcc = 0.92; MVD-A vs TVA, pcc = 0.78; MVD-B vs TVA, pcc = 0.76). The myeloma cases had significantly higher angiogenesis parameters when compared with controls (Kruskall-Wallis test, P < 0.001). ''Complete responders'' (n = 38/110) had significant lower angiogenesis (Mann-Whitney U test, P < 0.001) than ''nonresponders'' (n = 72/110). On treatment follow-up ''rapid disease progressors'' had the highest levels of angiogenesis (mean rank for MVD-A = 84.7, MVD-B = 82.1, and TVA = 81.1). On multivariate (logistic regression) analysis, factors found to have independent prognostic significance in complete responders (adjusted odd ratio (95% CI, P value)] were: (a) MVD-B grade I [0.134 (0.10-0.16, P < 0.001)], (b) clinical substage A [0.163 (0.12-0.19, P = 0.008)], (c) Bartl's histological stage II & I [0.262 (0.2-0.32, P = 0.021)], (d) MVD-A grade I [0.28 (0.22-0.36, P = 0.03)], (e) b 2 microglobulin levels less than 3,400 ng/dl [0.31 (0.23-0.42, P = 0.04)]. Kaplan-Meier survival analysis for myeloma-related death (n = 16) shows a mean survival time (in months) of 24.75; SE = 3; 95% CI = 21-28. We conclude that MVD (particularly MVD-B) is a very good predictor for the complete response in patients of MM and should be done routinely on bone marrow biopsies. Am.
American Journal of Clinical Pathology, 1996
The relationship between two cellular prognostic parameters of multiple myeloma, the plasma cell labeling index (LI%) and bone marrow histology was studied. The LI% as the percentage of monoclonal plasma cells in the S-phase was measured by bromodeoxyuridine incorporation using the anti-bromodeoxyuridine antibody BU-1. The histologic classification was based on six plasma cell types that allow prognostic grading as the Marschalko, small cell, cleaved, polymorphous, asynchronous, and blastic types. The biopsies also were used for estimating the degree of infiltration, /^-microglobulin (IMx assay, Abbott, North Chicago, IL), the most significant serum parameter for myeloma also was measured for comparison. Bone marrow specimens and sera were obtained from 50 myeloma patients. Fourteen patients with smoldering myeloma were characterized by well-differentiated Marschalko or small cell type cells, a low LI%, and a low /^-microglobulin concentration. Considering all myeloma patients, plasma cell type and degree of infiltration showed a significant correla-From the
PARIPEX INDIAN JOURNAL OF RESEARCH, 2021
Introduction: Myeloma is characterized by the neoplastic proliferation of a single plasma cell of terminally differentiated B lymphoid cells that produces monoclonal immunoglobulin. Myeloma often manifests with many clinical symptoms and organ damage, including anemia, hypercalcemia, renal insufficiency, lytic bone lesion, hyperviscosity, amyloidosis, and infection. Background : Bone marrow examination gold standard in establishing the diagnosis of multiple myeloma along with clinical ,radiological and laboratory parameters the histological criteria for staging will help in determining the prognosis. Objective: To correlate the clinical and laboratory parameter in the diagnosis and staging of multiple myeloma in the SMS Medical College & Hospital Jaipur. Materials and Methods: All haematological sample of multiple myeloma and histopathology specimens received of respective tissues in Department of Pathology SMS Medical College & Attached Hospital, Jaipur. Study from 2019 to 2020. Re...
Biochemical profile of multiple myeloma about 50 cases
GSC Advanced Research and Reviews, 2021
Multiple myeloma (MM) is a clonal proliferation of plasma cells invading the bone marrow and secreting monoclonal immunoglobulin. In order to study the epidemiological and biological and biochemical characteristics of MM, we carried out a retrospective work on a cohort of 50 cases collected at the Avicenna Military Hospital in Marrakesh, during a period of 5 years (from January 2013 to December 2017). Our study included 32 men (64%) and 18 women (36%), with an average age of 60.6 years, with extremes at 44 and 87 years. The circumstances of discovery were dominated by bone pain and alteration in general condition, which are revealing in more than 65% of cases. Biologically: the sedimentation rate was accelerated in 86% of cases, a monoclonal peak appearance was revealed on serum proteins electrophoresis in 88%of cases, most often located in the γ zone (64%), a predominance of the Ig G isotype (64%), and kappa light chains in 60% of cases, Bence Jones protein (BJP) was found in 7 pat...
Multidisciplinary Cancer Investigation, 2018
Introduction: Utilizing lower limit of bone marrow plasma cells (BMPCs) is the main existing criterion to diagnose multiple myeloma (MM). According to the revised international myeloma working group (IMWG) diagnostic criteria, the value of 10% is agreed among experts as the cut off level for diagnosis. Symptomatic patients with BMPC above this value are identified as definite cases of MM. However, there are MM patients who have BMPC of less than 10%. Therefore, the above-mentioned cut off point could delay the diagnosis, which in turn results in adverse effects in patients' clinical course. Case Presentation: The current study represented data from consecutive patients with 5%-10% BMPC at our center from 2004 to 2013. MM existed among patients, as expected. This series provides a quantitative approximation of MM prevalence in the studied cases. Conclusion: The reported patients' status demonstrated the limitations of the abovementioned cutoff criterion to diagnose myeloma, and emphasizes the importance of employing further diagnostic procedures in patients with marginal amounts of BMPC and high clinical suspicion. It is shown that supplementary examination is especially required for two subgroups of patients with certain clinical and laboratory characteristics.
Multidisciplinary Cancer Investigation
Introduction: Utilizing lower limit of bone marrow plasma cells (BMPCs) is the main existing criterion to diagnose multiple myeloma (MM). According to the revised international myeloma working group (IMWG) diagnostic criteria, the value of 10% is agreed among experts as the cut off level for diagnosis. Symptomatic patients with BMPC above this value are identified as definite cases of MM. However, there are MM patients who have BMPC of less than 10%. Therefore, the above-mentioned cut off point could delay the diagnosis, which in turn results in adverse effects in patients' clinical course. Case Presentation: The current study represented data from consecutive patients with 5%-10% BMPC at our center from 2004 to 2013. MM existed among patients, as expected. This series provides a quantitative approximation of MM prevalence in the studied cases. Conclusion: The reported patients' status demonstrated the limitations of the abovementioned cutoff criterion to diagnose myeloma, and emphasizes the importance of employing further diagnostic procedures in patients with marginal amounts of BMPC and high clinical suspicion. It is shown that supplementary examination is especially required for two subgroups of patients with certain clinical and laboratory characteristics.