Wound-healing Activity of the Aqueous Leaf Extract and Fractions of Ficus exasperata (Moraceae) and its Safety Evaluation on Albino Rats (original) (raw)

Wound healing activity of the hydro alcoholic extract of Ficus religiosa leaves in rats

The Internet Journal of Alternative Medicine, 2009

The leaves of Ficus religiosa are used traditionally in the folklore for the treatment of various kinds of wounds. The present study was undertaken to verify the effect of hydro alcoholic extract of Ficus religiosa leaves on experimentally induced wounds in rats in excision wound, incision wound and burn wound models. A formulation of leaves extract was prepared in emulsifying ointment at a concentration of 5% & 10% and applied to the wounds. In the excision wound and the burn wound models the extract showed significant decrease in the period of epithelization and in wound contraction (50%). A significant increase in the breaking strength was observed in the incision wound model when compared to the control. The result suggests that leaf extract of Ficus religiosa (both 5% and 10%) applied topically possess dose dependent wound healing activity.

Wound healing activity of the hydro alcoholic extract of Ficus religiosa leaves in rats

The Internet Journal of Alternative Medicine, 2009

The leaves of Ficus religiosa are used traditionally in the folklore for the treatment of various kinds of wounds. The present study was undertaken to verify the effect of hydro alcoholic extract of Ficus religiosa leaves on experimentally induced wounds in rats in excision wound, incision wound and burn wound models. A formulation of leaves extract was prepared in emulsifying ointment at a concentration of 5% & 10% and applied to the wounds. In the excision wound and the burn wound models the extract showed significant decrease in the period of epithelization and in wound contraction (50%). A significant increase in the breaking strength was observed in the incision wound model when compared to the control. The result suggests that leaf extract of Ficus religiosa (both 5% and 10%) applied topically possess dose dependent wound healing activity.

Promotion of wound healing in mice using a formulation of Ficus sur Forssk aqueous leaf extract

2018

1 Department of Pharmacology and Toxicology, National Institute for Pharmaceutical Research and Development, Idu, Abuja, Nigeria. 2 Department of Raw Materials Research and Pharmaceutical Technology, National Institute for Pharmaceutical Research and Development, Idu, Abuja, Nigeria. 3 Department of Pharmacology and Therapeutics, Faculty of Pharmaceutical Sciences, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.

Comparative study of wound-healing effect of bark extracts of Ficus religiosa & Ficus benghalensis by mice model

2019

The aim of the study was the comparative study of the wound healing potential of the bark of Ficus religiosa and Ficus benghalensis. The locally available plants like Ficus religiosa and Ficus bengalensis have the capability to cure diseases traditionally since long years, due to this reasons these two plants were selected for the study. It was found that ethanol and hydroalcoholic extraction of two plants that is Ficus religiosa and Ficus benghalensis showed high wound healing activity. The plant extracts were used to study their phytochemical compositions i.e. total phenols contents, flavonoids contents, terpenoids content and proteins contents. Most of the biologically active phytochemical presents in the ethanol extract. The results obtained that Wound healing potential of the bark of Ficus religiosa is higher than the bark of Ficus benghalensis. The antimicrobial activity was evaluated by well diffusion method while wound healing effect in rats was evaluated using the in vitro ...

Potential of Ethanolic Extract of Ficus Benghalensis on Open Wounds and Inflammation

International Journal of Pharmacological Research, 2012

The present study deals with evaluation of antioxidant, wound healing and anti-inflammatory activity of ethanolic extract of Curcuma longa Linn rhizomes. The ethanolic extract prepared by maceration technique was subjected to screen for antioxidant activity using DPPH radical scavenging method and wound healing activity using incision, excision, histopathological and dead space wound model and the study was supported with evaluation of granuloma tissue to estimate hydroxyproline content and histopathological evaluation. The anti-inflammatory study was carried out by using carageenan induced rat paw odema method. The tested extract of different dilutions in range 200µg/ml to 1000 µg/ml shows activity in range of 9.34% to 18.55%. Significant increase in wound closure rate, skin breaking strength, granuloma breaking strength was observed. The hydroxyproline content was also increased with decrease in scar area. The initial healing action might be due to increased collagen deposition and better alignment, with the obtained results it can be concluded that Curcuma longa extract has significant wound healing activity and initial healing may be due to presence of some terpenoids and antimicrobial agents. The extract shows prominent anti-inflammatory activity as compared to that of standard (Ibuprofen gel). The extract shows good anti-inflammatory activity on carageenan induced rat paw odema method.

Chemical findings and in vitro biological studies to uphold the use of Ficus exasperata Vahl leaf and stem bark

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 2017

Ficus exasperata Vahl, commonly known as sandpaper, is a terrestrial Afro-tropical tree used in popular medicine. Despite the existence of some works on the biological activities of this species, its chemical composition is still poorly known. The aim of this study was to extend the knowledge on the phytochemistry and biological properties of this species. Aqueous extracts from F. exasperata leaves and stem bark were analysed. Thirty-one phenolic compounds, comprising cinnamoyl derivatives, flavonoid-O-glycosides, flavonoid-mono-C-glycosides, flavonoid-di-C-glycosides and one furanocoumarin, were determined by HPLC-DAD-ESI/MSn and UPLC-ESI-QTOF-MS, 26 of them being reported for the first time in this species. The profile of organic acids, characterized by HPLC-UV, was also reported for the first time. The best radical scavenging activity was observed for the aqueous extract from leaves (IC50 values of 222.5, 510.0 and 50.0 μg/mL against DPPH•, •NO and O2•-, respectively). In additio...

Anti-inflammatory, antioxidant and antimicrobial activity of the stem bark extract and fractions of Ficus exasperata Vahl. (Moraceae)

JOURNAL OF PHARMACOGNOSY AND PHYTOCHEMISTRY , 2013

Ficus exasperata is used in various ethnomedicines for the treatment of pain, inflammatory diseases, wounds and abscesses. Different solvent extracts of the stem bark of F. exasperata, which is rarely studied, were tested for anti-inflammatory, antioxidant and antimicrobial activities. Antiinflammatory activity of the extract was evaluated using the carrageenan induced foot pad eodema model in seven day old chicks. In the test for antioxidant activity, a DPPH radical scavenging assay was performed. Antimicrobial evaluation of the extract was done using the agar well diffusion and micro-dilution assays. The hydro-alcoholic stem bark extract (30-300mg/kg, p.o) dose dependently inhibited carrageenan induced foot eodema with maximum inhibition of 68.57±3.342% (300mg/kg) which was comparable to that of diclofenac (71.56±3.43% at 100mg/kg) and dexamethasone (74.53±5.21% at 3mg/kg). The extract also showed antibacterial activity against both gram positive and negative organisms. The most sensitive pathogenic strains inhibited by the extract were Pseudomonas aeruginosa, Salmonella typhi, Klebsiella pneumonae and Staphylococus aureus with respective zones of inhibition of 14.33±0.33, 12.33±0.33, 17.33±0.67 and 15.0±0.58mm. The MIC's ranged between 250-1000µg/ml. The lowest MIC of 250µg/ml was recorded for Klebsiella pneumonae. The anti-inflammatory activities and antimicrobial activities of the stem bark were highest in it chloroform fraction. The activities shown by the extract in this study may be due to the presence of phytosterols, triterpenes, tannins, flavonoids and glycosides which were found in the phytochemical screening of the powdered stem bark. The present study validates the traditional uses of the plant in the treatment of inflammation and infectious diseases.

Antimicrobial activity of Ficus exasperata (Vahl) leaf extract in clinical isolates and its development into herbal tablet dosage form

Journal of Medicinal Plants for Economic Development

A number of plants, including Ficus exasperata (Vahl), have been used in traditional medicine for many years because of their antimicrobial properties (Sofowora 1993). Ficus exasperata belongs to the family Moraceae, with 800 species occurring in the tropical part of the world, chiefly in Indomalaya and Polynesia (Odunbaku, Ilusanya & Akasoro 2008). Ficus species are plants well known all over the world as 'fig plant'. Ficus exasperata, 'Eepin' in Yoruba language, western Nigeria, is a medicinal plant locally known as 'sand paper plant' and used for treating eyesores, stomach pains, ring worm infection (Abbiw 1990), leprosy, controlling bleeding and easing childbirth (Burkill 1997). In Nigeria, the young leaves of F. exasperata are prescribed as a common antiulcer remedy in addition to various pharmacological actions, such as antidiabetic, antihypertensive, lipid-lowering and antimicrobial activities, which have been reported for the extract (Buniyamin, Eric & Fabian 2007; Sonibare, Isiaka & Taruka 2006). Background: Ficus exasperata (Vahl) from the family Moraceae is a herb of ethnobotanical importance with antimicrobial properties. The past studies have been on the phytochemistry and pharmacological activities of the plant, but there is a need to formulate this valuable herb into tablet dosage form to offer standardisation. Aim: The aim of this study was to assess the antimicrobial activity of F. exasperata leaf extract (extract) and formulate it into herbal tablet dosage form.

EVALUATION OF ANTIOXIDANT, WOUND HEALING AND ANTI-INFLMMATORY ACTIVITY OF ETHANOLIC EXTRACT OF LEAVES OF FICUS RELIGIOSA

Int J Pharm Sci Res, 2010

The present study deals with evaluation of antioxidant, wound healing and anti-inflammatory activity of ethanolic extract of leaves of Ficus religiosa. The ethanolic extract prepared by maceration technique was subjected to screen for antioxidant activity using DPPH radical scavenging method and wound healing activity using incision, excision, histopathological and dead space wound model and the study was supported with evaluation of granuloma tissue to estimate hydroxyproline content and histopathological evaluation. The anti-inflammatory study was carried out by using carageenan induced rat paw odema method. The tested extract of different dilutions in range 200µg/ml to 1000 µg/ml shows activity in range of 6.34% to 13.35%. Significant increase in wound closure rate, skin breaking strength, granuloma breaking strength was observed. The hydroxyproline content was also increased with decrease in scar area. The initial healing action might be due to increased collagen deposition and better alignment, with the obtained results it can be concluded that Ficus religiosa extract has significant wound healing activity and initial healing may be due to presence of glycosides and tannins. The extract shows prominent anti-inflammatory activity as compared to that of standard (Ibuprofen gel). The extract shows good anti-inflammatory activity on carageenan induced rat paw odema method.

Effects of herbal ointment prepared from ethanolic extract of Ficus racemosa leaves on wound causing bacteria

The Ficus racemosa plant is a member of the Moraceae family. It is used to treat a variety of illnesses, such as diabetes, liver problems, diarrhoea, inflammatory ailments, haemorrhoids, respiratory, and urinary illnesses. Pharmacological research on F. racemosa has examined its potential for antidiabetic, antipyretic, anti-inflammatory, antitussive, hepatoprotective, and antibacterial effects. In this paper, an effort was undertaken to examine the phytochemical components and antibacterial effects of the ethanolic extract on bacteria that cause wounds. The gel made from an ethanolic extract of Ficus racemosa works best against S. aureus, somewhat against E. coli, and least well against Klebsiella spp. That unmistakably demonstrated Ficus racemosa has antibacterial effect against wound microorganisms. Thus, we draw the conclusion that Ficus racemosa offers additional herbal treatments for wound care.